Nos2 ELISA Kit for Mouse(Mus musculus) | EIAab.comNos2 ELISA Kit for Mouse(Mus musculus)

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Nos2 (Gene name),

Nitric oxide synthase, inducible (Protein name ), NOS2_MOUSE from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

Citations (5)

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Gene name:

Nos2(Inosl);

Protein name:

Nitric oxide synthase, inducible;

Alternative:

Macrophage NOS(MAC-NOS);Inducible NO synthase(Inducible NOS;iNOS);Peptidyl-cysteine S-nitrosylase NOS2;NOS type II;

Organism:

Mouse (Mus musculus).

General Annotation

Sub Unit:

Homodimer. Binds SLC9A3R1.

Function:

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.

Subcellular Location:

N/A

Protein Attributes:

Sequence length:

1144

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

550:

600:

650:

700:

750:

800:

850:

900:

950:

1000:

1050:

1100:

1144:

EEQVEDYFFQ | LKSQKRYHED | IFGAVFSYGA | KKGSALEEPK | ATRL

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

String:

P29477

Pfam:

PF00667

SMR:

P29477

KEGG:

mmu:18126

UniGene:

Mm.2893

Uniprot:

P29477

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ELISA Kit for Mouse Nitric oxide synthase, inducible

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ELISA Kit for Mouse Nitric oxide synthase, inducible

Cat.:

E0837r

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96T

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ELISA Kit for Mouse Nitric oxide synthase, inducible

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E0837m

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96T

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ELISA Kit for Mouse Nitric oxide synthase, inducible

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E0837h

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96T

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ELISA Kit for Mouse Nitric oxide synthase, inducible

Cat.:

E0837b

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96T

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ELISA Kit for Mouse Nitric oxide synthase, inducible

Cat.:

E0837p

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ELISA Kit for Mouse Nitric oxide synthase, inducible

Cat.:

E0837d

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96T

ELISA Kit for Mouse Nitric oxide synthase, inducible

Cat.:

E0837c

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96T

CLIA Kit for Mouse Nitric oxide synthase, inducible

Cat.:

U0837m

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96T

CLIA Kit for Mouse Nitric oxide synthase, inducible

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U0837d

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96T

CLIA Kit for Mouse Nitric oxide synthase, inducible

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CLIA Kit for Mouse Nitric oxide synthase, inducible

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CLIA Kit for Mouse Nitric oxide synthase, inducible

Cat.:

U0837b

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96T

CLIA Kit for Mouse Nitric oxide synthase, inducible

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U0837c

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96T

CLIA Kit for Mouse Nitric oxide synthase, inducible

Cat.:

U0837r

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Looking for manual.

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CLIA Kit for Mouse Nitric oxide synthase, inducible

Cat.:

U0837h

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96T

Polyclonal Antibody for Mouse Nitric oxide synthase, inducible

Cat.:

P0837Rb-h

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40ug/0.2ml

Polyclonal Antibody for Mouse Nitric oxide synthase, inducible

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P0837Rb-m

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40ug/0.2ml

Polyclonal Antibody for Mouse Nitric oxide synthase, inducible

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P0837Rb-r

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40ug/0.2ml

Monoclonal Antibody for Mouse Nitric oxide synthase, inducible

Protein for Mouse Nitric oxide synthase, inducible

Cat.:

R0837h

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Packing:

1mg

Purity:

>90%

Protein for Mouse Nitric oxide synthase, inducible

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

12% SDS-PAGE analysis

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

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Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
Saliva(n=3)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Citations

[1].

"Innate Signaling Promotes Formation of Regulatory Nitric Oxide–Producing Dendritic Cells Limiting T-Cell Expansion in Experimental Autoimmune Myocardi"

Gabriela Kania; Stefanie Siegert; Silvia Behnke; Rafael Prados-Rosales; Arturo Casadevall; Thomas F. Lüscher; Sanjiv A. Luther; Manfred Kopf; Urs Eriksson; Przemyslaw Blyszczuk

From:Circulation

[2].

"Glycyrrhizin Treatment Is Associated with Attenuation of Lipopolysaccharide-Induced Acute Lung Injury by Inhibiting Cyclooxygenase-2 and Inducible..."

Yun-Feng Ni, Jian-Ke Kuai, Zi-Fan Lu, Guo-Dong Yang, Hai-Yan Fu, Jian Wang, Feng Tian, Xiao-Long Yan, Ya-Chao Zhao, Yun-Jie Wang, and Tao Jiang,

From:Journal of Surgical Research

[3].

"Cyclooxygenase-1 Serves a Vital Hepato-Protective Function in Chemically Induced Acute Liver Injury"

J Xiao, EC. Liong, H Huang, et al.

From:Toxicological sciences

[4].

"In vitro morphology, viability and cytokine secretion of uterine telocyte-activated mouse peritoneal macrophages"

Chi C, Jiang X J, Su L, et al.

From:Journal of cellular and molecular medicine

[5].

"Innate Signaling Promotes Formation of Regulatory Nitric Oxide–Producing Dendritic Cells Limiting T-Cell Expansion in Experimental Autoimmune..."

Kania G, Siegert S, Behnke S, et al.

From:Circulation

References

1.

"Cloning and characterization of inducible nitric oxide synthase from mouse macrophages."
Xie Q.-W. , Cho H.J. , Calaycay J. , Mumford R.A. , Swiderek K.M. , Lee T.D. , Ding A. , Troso T. , Nathan C.
Science256:225-228(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
2.

"Cloned and expressed macrophage nitric oxide synthase contrasts with the brain enzyme."
Lowenstein C.J. , Glatt C.S. , Bredt D.S. , Snyder S.H.
Proc. Natl. Acad. Sci. U.S.A.89:6711-6715(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
3.

"Molecular cloning and functional expression of an inducible nitric oxide synthase from a murine macrophage cell line."
Lyons C.R. , Orloff G.J. , Cunningham J.M.
J. Biol. Chem.267:6370-6374(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
4.

"Role of NF-kappa B in the regulation of inducible nitric oxide synthase in an MTAL cell line."
Kone B.C. , Schwoebel J. , Turner P. , Mohaupt M.G. , Cangro C.B.
Am. J. Physiol.269:F718-F729(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
5.

"Sequence polymorphisms in the chemokines Scya1 (TCA-3), Scya2 (monocyte chemoattractant protein (MCP)-1), and Scya12 (MCP-5) are candidates for eae7, a locus controlling susceptibility to monophasic remitting/nonrelapsing experimental allergic encephalomyelitis."
Teuscher C. , Butterfield R.J. , Ma R.Z. , Zachary J.F. , Doerge R.W. , Blankenhorn E.P.
J. Immunol.163:2262-2266(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];VARIANTS ARG-211; LEU-967 AND PHE-968
strain: B10.S/J.
strain: BALB/cByJ.
strain: DBA/2J.
strain: NOD/LtJ.
strain: SJL/J.
tissue: Spleen.
6.

"Genomic structure of the murine inducible nitric oxide synthase (i-NOS) gene."
Coge F. , Levacher B. , Rique H. , Leopold O. , Boutin J.A. , Galizzi J.-P.
Submitted (2001-10) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
strain: CD-1.
7.

"Mouse inducible nitric oxide synthase mRNA."
Hagiwara K. , Endo Y. , Xin H. , Takahashi M. , Huqun X. , Nukiwa T.
Submitted (2002-03) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
strain: ICR.
8.

"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M. , Goodstadt L. , Hillier L.W. , Zody M.C. , Goldstein S. , She X. , Bult C.J. , Agarwala R. , Cherry J.L. , DiCuccio M. , Hlavina W. , Kapustin Y. , Meric P. , Maglott D. , Birtle Z. , Marques A.C. , Graves T. , Zhou S. , more...
PLoS Biol.7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
strain: C57BL/6J.
9.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
strain: NMRI.
tissue: Mammary tumor.
10.

Lubec G. , Sunyer B. , Chen W.-Q.
Submitted (2009-01) to the UniProtKB

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 733-744;IDENTIFICATION BY MASS SPECTROMETRY
strain: OF1.
tissue: Hippocampus.
11.

"The mode of action of aspirin-like drugs: effect on inducible nitric oxide synthase."
Amin A.R. , Vyas P. , Attur M. , Leszczynska-Piziak J. , Patel I.R. , Weissmann G. , Abramson S.B.
Proc. Natl. Acad. Sci. U.S.A.92:7926-7930(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: EFFECT OF ASPIRIN
tissue: Macrophage.
12.

"Inducible nitric oxide synthase binds, S-nitrosylates, and activates cyclooxygenase-2."
Kim S.F. , Huri D.A. , Snyder S.H.
Science310:1966-1970(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION AS NITROSYLASE
13.

"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M. , English L. , Lemieux S. , Courcelles M. , Desjardins M. , Thibault P.
Immunity30:143-154(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
14.

"The circadian deadenylase Nocturnin is necessary for stabilization of the iNOS mRNA in mice."
Niu S. , Shingle D.L. , Garbarino-Pico E. , Kojima S. , Gilbert M. , Green C.B.
PLoS ONE6:E26954-E26954(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INDUCTION
15.

"The structure of nitric oxide synthase oxygenase domain and inhibitor complexes."
Crane B.R. , Arvai A.S. , Gachhui R. , Wu C. , Ghosh D.K. , Getzoff E.D. , Stuehr D.J. , Tainer J.A.
Science278:425-431(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 116-496
16.

"Structure of nitric oxide synthase oxygenase dimer with pterin and substrate."
Crane B.R. , Arvai A.S. , Ghosh D.K. , Wu C. , Getzoff E.D. , Stuehr D.J. , Tainer J.A.
Science279:2121-2126(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 77-496
17.

"Inducible nitric oxide synthase: role of the N-terminal beta-hairpin hook and pterin-binding segment in dimerization and tetrahydrobiopterin interaction."
Ghosh D.K. , Crane B.R. , Ghosh S. , Wolan D. , Gachhui R. , Crooks C. , Presta A. , Tainer J.A. , Getzoff E.D. , Stuehr D.J.
EMBO J.18:6260-6270(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS) OF 77-496
18.

"N-terminal domain swapping and metal ion binding in nitric oxide synthase dimerization."
Crane B.R. , Rosenfeld R.J. , Arvai A.S. , Ghosh D.K. , Ghosh S. , Tainer J.A. , Stuehr D.J. , Getzoff E.D.
EMBO J.18:6271-6281(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS) OF 77-499
19.

"Structures of the N(omega)-hydroxy-L-arginine complex of inducible nitric oxide synthase oxygenase dimer with active and inactive pterins."
Crane B.R. , Arvai A.S. , Ghosh S. , Getzoff E.D. , Stuehr D.J. , Tainer J.A.
Biochemistry39:4608-4621(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS) OF 77-496
20.

"Structures of tetrahydrobiopterin binding-site mutants of inducible nitric oxide synthase oxygenase dimer and implicated roles of Trp457."
Aoyagi M. , Arvai A.S. , Ghosh S. , Stuehr D.J. , Tainer J.A. , Getzoff E.D.
Biochemistry40:12826-12832(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF TRP-457 MUTANTS
21.

"Conformational changes in nitric oxide synthases induced by chlorzoxazone and nitroindazoles: crystallographic and computational analyses of inhibitor potency."
Rosenfeld R.J. , Garcin E.D. , Panda K. , Andersson G. , Aberg A. , Wallace A.V. , Morris G.M. , Olson A.J. , Stuehr D.J. , Tainer J.A. , Getzoff E.D.
Biochemistry41:13915-13925(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS) OF 77-496
22.

"Crystal structures of cyanide complexes of P450cam and the oxygenase domain of inducible nitric oxide synthase -- structural models of the short-lived oxygen complexes."
Fedorov R. , Ghosh D.K. , Schlichting I.
Arch. Biochem. Biophys.409:25-31(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 77-495

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