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Index > Protein center > NOS3(Gene name) > Bovine
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  • NOS3 (Gene name),
  • Nitric oxide synthase, endothelial (Protein name ),  NOS3_BOVIN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Gene name:
    NOS3;
    Protein name:
    Nitric oxide synthase, endothelial;
    Alternative:
    EC-NOS;Constitutive NOS(cNOS);NOS type III(NOSIII);Endothelial NOS(eNOS);
    Organism:
    Bovine (Bos taurus). 
    General Annotation
    Sub Unit:
    Homodimer. Interacts with NOSIP and NOSTRIN.
    Function:
    Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
    Subcellular Location:
    Cell membrane Membrane caveola Cytoplasm cytoskeleton Golgi apparatus Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
    Protein Attributes:
    Sequence length:
    1205
    Sequence:
    50:
    MGNLKSVGQE | PGPPCGLGLG | LGLGLCGKQG | PASPAPEPSR | APAPATPHAP | 
    100:
    DHSPAPNSPT | LTRPPEGPKF | PRVKNWELGS | ITYDTLCAQS | QQDGPCTPRC | 
    150:
    CLGSLVLPRK | LQTRPSPGPP | PAEQLLSQAR | DFINQYYSSI | KRSGSQAHEE | 
    200:
    RLQEVEAEVA | STGTYHLRES | ELVFGAKQAW | RNAPRCVGRI | QWGKLQVFDA | 
    250:
    RDCSSAQEMF | TYICNHIKYA | TNRGNLRSAI | TVFPQRAPGR | GDFRIWNSQL | 
    300:
    VRYAGYRQQD | GSVRGDPANV | EITELCIQHG | WTPGNGRFDV | LPLLLQAPDE | 
    350:
    APELFVLPPE | LVLEVPLEHP | TLEWFAALGL | RWYALPAVSN | MLLEIGGLEF | 
    400:
    SAAPFSGWYM | STEIGTRNLC | DPHRYNILED | VAVCMDLDTR | TTSSLWKDKA | 
    450:
    AVEINLAVLH | SFQLAKVTIV | DHHAATVSFM | KHLDNEQKAR | GGCPADWAWI | 
    500:
    VPPISGSLTP | VFHQEMVNYI | LSPAFRYQPD | PWKGSATKGA | GITRKKTFKE | 
    550:
    VANAVKISAS | LMGTLMAKRV | KATILYASET | GRAQSYAQQL | GRLFRKAFDP | 
    600:
    RVLCMDEYDV | VSLEHEALVL | VVTSTFGNGD | PPENGESFAA | ALMEMSGPYN | 
    650:
    SSPRPEQHKS | YKIRFNSVSC | SDPLVSSWRR | KRKESSNTDS | AGALGTLRFC | 
    700:
    VFGLGSRAYP | HFCAFARAVD | TRLEELGGER | LLQLGQGDEL | CGQEEAFRGW | 
    750:
    AKAAFQASCE | TFCVGEEAKA | AAQDIFSPKR | SWKRQRYRLS | TQAEGLQLLP | 
    800:
    GLIHVHRRKM | FQATVLSVEN | LQSSKSTRAT | ILVRLDTAGQ | EGLQYQPGDH | 
    850:
    IGICPPNRPG | LVEALLSRVE | DPPPPTESVA | VEQLEKGSPG | GPPPSWVRDP | 
    900:
    RLPPCTLRQA | LTFFLDITSP | PSPRLLRLLS | TLAEEPSEQQ | ELETLSQDPR | 
    950:
    RYEEWKWFRC | PTLLEVLEQF | PSVALPAPLL | LTQLPLLQPR | YYSVSSAPNA | 
    1000:
    HPGEVHLTVA | VLAYRTQDGL | GPLHYGVCST | WLSQLKTGDP | VPCFIRGAPS | 
    1050:
    FRLPPDPYVP | CILVGPGTGI | APFRGFWQER | LHDIESKGLQ | PAPMTLVFGC | 
    1100:
    RCSQLDHLYR | DEVQDAQERG | VFGRVLTAFS | REPDSPKTYV | QDILRTELAA | 
    1150:
    EVHRVLCLER | GHMFVCGDVT | MATSVLQTVQ | RILATEGDME | LDEAGDVIGV | 
    1200:
    LRDQQRYHED | IFGLTLRTQE | VTSRIRTQSF | SLQERHLRGA | VPWAFDPPGP | 
    1205:
    DTPGP
    3D Structure:
    N/A
    Predicted Eptitope:
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    EIAab Sequence  Vaild Sequence:
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    Related Databases
    SMR:
    UniGene:
    Pfam:
    String:
    Uniprot:
     
    FOR
    ELISA Kit for Bovine Nitric oxide synthase, endothelial
    Cat.:
    E0815r
    Price:
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    MSDS:
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    Packing:
    96T
    Range:
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    ELISA Kit for Bovine Nitric oxide synthase, endothelial
    Cat.:
    E0815h
    Price:
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    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
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    ELISA Kit for Bovine Nitric oxide synthase, endothelial
    Cat.:
    E0815d
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    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
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    ELISA Kit for Bovine Nitric oxide synthase, endothelial
    Cat.:
    E0815p
    Price:
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    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Bovine Nitric oxide synthase, endothelial
    Cat.:
    E0815m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Bovine Nitric oxide synthase, endothelial
    Cat.:
    E0815b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Bovine Nitric oxide synthase, endothelial
    Cat.:
    U0815h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Bovine Nitric oxide synthase, endothelial
    Cat.:
    U0815r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Bovine Nitric oxide synthase, endothelial
    Cat.:
    U0815d
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Bovine Nitric oxide synthase, endothelial
    Cat.:
    U0815p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Bovine Nitric oxide synthase, endothelial
    Cat.:
    U0815m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Bovine Nitric oxide synthase, endothelial
    Cat.:
    U0815b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Bovine Nitric oxide synthase, endothelial
    Cat.:
    P0815Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Bovine Nitric oxide synthase, endothelial
    Cat.:
    P0815Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Bovine Nitric oxide synthase, endothelial
    Polyclonal Antibody for Bovine Nitric oxide synthase, endothelial
    Cat.:
    P0815Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Bovine Nitric oxide synthase, endothelial
    Polyclonal Antibody for Bovine Nitric oxide synthase, endothelial
    Monoclonal Antibody for Bovine Nitric oxide synthase, endothelial
    Monoclonal Antibody for Bovine Nitric oxide synthase, endothelial
    Monoclonal Antibody for Bovine Nitric oxide synthase, endothelial
    Monoclonal Antibody for Bovine Nitric oxide synthase, endothelial
    Monoclonal Antibody for Bovine Nitric oxide synthase, endothelial
    Monoclonal Antibody for Bovine Nitric oxide synthase, endothelial
    Protein for Bovine Nitric oxide synthase, endothelial
    Protein for Bovine Nitric oxide synthase, endothelial
    Protein for Bovine Nitric oxide synthase, endothelial
    Protein for Bovine Nitric oxide synthase, endothelial
    Protein for Bovine Nitric oxide synthase, endothelial
    Protein for Bovine Nitric oxide synthase, endothelial

    R&D Technical Data
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    s
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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

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    Recovery
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    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    Saliva(n=3)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

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    References
    1. 1.
      "Endothelial nitric oxide synthase: molecular cloning and characterization of a distinct constitutive enzyme isoform."
      Lamas S. , Marsden P.A. , Li G.K. , Tempst P. , Michel T.
      Proc. Natl. Acad. Sci. U.S.A.89:6348-6352(1992) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
    2. 2.
      "Molecular cloning and characterization of the constitutive bovine aortic endothelial cell nitric oxide synthase."
      Nishida K. , Harrison D.G. , Navas J.P. , Fisher A.A. , Dockery S.P. , Uematsu M. , Nerem R.M. , Alexander R.W. , Murphy T.J.
      J. Clin. Invest.90:2092-2096(1992) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
    3. 3.
      "Molecular cloning and expression of a cDNA encoding endothelial cell nitric oxide synthase."
      Sessa W.C. , Harrison J.K. , Barber C.M. , Zeng D. , Durieux M.E. , D'Angelo D.D. , Lynch K.R. , Peach M.J.
      J. Biol. Chem.267:15274-15276(1992) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
      tissue: Aortic endothelium.
    4. 4.
      "Endothelial nitric oxide synthase. N-terminal myristoylation determines subcellular localization."
      Busconi L. , Michel T.
      J. Biol. Chem.268:8410-8413(1993) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: MYRISTOYLATION AT GLY-2
    5. 5.
      "Mutagenesis of palmitoylation sites in endothelial nitric oxide synthase identifies a novel motif for dual acylation and subcellular targeting."
      Robinson L.J. , Michel T.
      Proc. Natl. Acad. Sci. U.S.A.92:11776-11780(1995) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PALMITOYLATION AT CYS-15 AND CYS-26
    6. 6.
      "Shear stress stimulates phosphorylation of eNOS at Ser(635) by a protein kinase A-dependent mechanism."
      Boo Y.C. , Hwang J. , Sykes M. , Michell B.J. , Kemp B.E. , Lum H. , Jo H.
      Am. J. Physiol.283:H1819-H1828(2002) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT THR-497; SER-635 AND SER-1179
    7. 7.
      "Aberrant cytoplasmic sequestration of eNOS in endothelial cells after monocrotaline, hypoxia, and senescence: live-cell caveolar and cytoplasmic NO imaging."
      Mukhopadhyay S. , Xu F. , Sehgal P.B.
      Am. J. Physiol.292:H1373-H1389(2007) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION
    8. 8.
      "Crystal structure of constitutive endothelial nitric oxide synthase: a paradigm for pterin function involving a novel metal center."
      Raman C.S. , Li H. , Martasek P. , Kral V. , Masters B.S.S. , Poulos T.L.
      Cell95:939-950(1998) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 67-482
    9. 9.
      "Mapping the active site polarity in structures of endothelial nitric oxide synthase heme domain complexed with isothioureas."
      Li H. , Raman C.S. , Martasek P. , Kral V. , Masters B.S.S. , Poulos T.L.
      J. Inorg. Biochem.81:133-139(2000) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.86 ANGSTROMS) OF 67-482
    10. 10.
      "Crystallographic studies on endothelial nitric oxide synthase complexed with nitric oxide and mechanism-based inhibitors."
      Li H. , Raman C.S. , Martasek P. , Masters B.S.S. , Poulos T.L.
      Biochemistry40:5399-5406(2001) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.93 ANGSTROMS)
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      "Crystal structure of nitric oxide synthase bound to nitro indazole reveals a novel inactivation mechanism."
      Raman C.S. , Li H. , Martasek P. , Southan G. , Masters B.S.S. , Poulos T.L.
      Biochemistry40:13448-13455(2001) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS)
    12. 12.
      "Implications for isoform-selective inhibitor design derived from the binding mode of bulky isothioureas to the heme domain of endothelial nitric-oxide synthase."
      Raman C.S. , Li H. , Martasek P. , Babu B.R. , Griffith O.W. , Southan G. , Masters B.S.S. , Poulos T.L.
      J. Biol. Chem.276:26486-26491(2001) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.93 ANGSTROMS)
    13. 13.
      "Structural basis for pterin antagonism in nitric-oxide synthase. Development of novel 4-oxo-pteridine antagonists of (6R)-5,6,7,8-tetrahydrobiopterin."
      Kotsonis P. , Frohlich L.G. , Raman C.S. , Li H. , Berg M. , Gerwig R. , Groehn V. , Kang Y. , Al-Masoudi N. , Taghavi-Moghadam S. , Mohr D. , Munch U. , Schnabel J. , Martasek P. , Masters B.S.S. , Strobel H. , Poulos T. , Matter H. , more...
      J. Biol. Chem.276:49133-49141(2001) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS)
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