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Index > Protein center > P4HA2(Gene name) > Human
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  • P4HA2 (Gene name),
  • Prolyl 4-hydroxylase subunit alpha-2 (Protein name ),  P4HA2_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • 3D
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Gene name:
    P4HA2(UNQ290/PRO330);
    Protein name:
    Prolyl 4-hydroxylase subunit alpha-2(4-PH alpha-2);
    Alternative:
    Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-2;
    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    Heterotetramer of two alpha-2 chains and two beta chains (the beta chain is the multi-functional PDI).
    Function:
    Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.
    Subcellular Location:
    Endoplasmic reticulum lumen
    Protein Attributes:
    Sequence length:
    535
    Sequence:
    50:
    MKLWVSALLM | AWFGVLSCVQ | AEFFTSIGHM | TDLIYAEKEL | VQSLKEYILV | 
    100:
    EEAKLSKIKS | WANKMEALTS | KSAADAEGYL | AHPVNAYKLV | KRLNTDWPAL | 
    150:
    EDLVLQDSAA | GFIANLSVQR | QFFPTDEDEI | GAAKALMRLQ | DTYRLDPGTI | 
    200:
    SRGELPGTKY | QAMLSVDDCF | GMGRSAYNEG | DYYHTVLWME | QVLKQLDAGE | 
    250:
    EATTTKSQVL | DYLSYAVFQL | GDLHRALELT | RRLLSLDPSH | ERAGGNLRYF | 
    300:
    EQLLEEEREK | TLTNQTEAEL | ATPEGIYERP | VDYLPERDVY | ESLCRGEGVK | 
    350:
    LTPRRQKRLF | CRYHHGNRAP | QLLIAPFKEE | DEWDSPHIVR | YYDVMSDEEI | 
    400:
    ERIKEIAKPK | LARATVRDPK | TGVLTVASYR | VSKSSWLEED | DDPVVARVNR | 
    450:
    RMQHITGLTV | KTAELLQVAN | YGVGGQYEPH | FDFSRNDERD | TFKHLGTGNR | 
    500:
    VATFLNYMSD | VEAGGATVFP | DLGAAIWPKK | GTAVFWYNLL | RSGEGDYRTR | 
    535:
    HAACPVLVGC | KWVSNKWFHE | RGQEFLRPCG | STEVD
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    String:
    MIM:
    Pfam:
    UniGene:
    KEGG:
    SMR:
    Uniprot:
     
    FOR
    ELISA Kit for Human 4-PH alpha-2
    Cat.:
    E10872m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    ELISA Kit for Human 4-PH alpha-2
    MSDS:
    Please sign in first.
    ELISA Kit for Human 4-PH alpha-2
    Cat.:
    E10872h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human 4-PH alpha-2
    Cat.:
    U10872h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human 4-PH alpha-2
    MSDS:
    Please sign in first.
    CLIA Kit for Human 4-PH alpha-2
    Cat.:
    U10872m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Human 4-PH alpha-2
    Polyclonal Antibody for Human 4-PH alpha-2
    Cat.:
    P10872Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human 4-PH alpha-2
    Monoclonal Antibody for Human 4-PH alpha-2
    Monoclonal Antibody for Human 4-PH alpha-2
    Monoclonal Antibody for Human 4-PH alpha-2
    Protein for Human 4-PH alpha-2
    Protein for Human 4-PH alpha-2
    Protein for Human 4-PH alpha-2

    R&D Technical Data
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

    s

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    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    Recovery
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    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Linearity
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    EDTA plasma(n=5)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    References
    1. 1.
      "Cloning of the human prolyl 4-hydroxylase alpha subunit isoform alpha(II) and characterization of the type II enzyme tetramer. The alpha(I) and alpha(II) subunits do not form a mixed alpha(I)alpha(II)beta2 tetramer."
      Annunen P. , Helaakoski T. , Myllyharju J. , Veijola J. , Pihlajaniemi T. , Kivirikko K.I.
      J. Biol. Chem.272:17342-17348(1997) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM IIB)
      tissue: Lung.
    2. 2.
      "Characterization of the human and mouse genes for the alpha subunit of type II prolyl 4-hydroxylase. Identification of a previously unknown alternatively spliced exon and its expression in various tissues."
      Nokelainen M. , Nissi R. , Kukkola L. , Helaakoski T. , Myllyharju J.
      Eur. J. Biochem.268:5300-5309(2001) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE (ISOFORMS IIA AND IIB)
    3. 3.
      "The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
      Clark H.F. , Gurney A.L. , Abaya E. , Baker K. , Baldwin D.T. , Brush J. , Chen J. , Chow B. , Chui C. , Crowley C. , Currell B. , Deuel B. , Dowd P. , Eaton D. , Foster J.S. , Grimaldi C. , Gu Q. , Hass P.E. , more...
      Genome Res.13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM IIA)
    4. 4.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    5. 5.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM IIA)
      tissue: Brain.
    6. 6.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
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