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Index > Protein center > CD274(Gene name) > Human
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  • CD274 (Gene name),
  • Programmed cell death 1 ligand 1 (Protein name ),  PD1L1_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • 3D
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Related Databases
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  • Gene name:
    CD274(B7H1;PDCD1L1;PDCD1LG1;PDL1);
    Protein name:
    Programmed cell death 1 ligand 1(PD-L1;PDCD1 ligand 1;Programmed death ligand 1);
    Alternative:
    B7 homolog 1(B7-H1);
    CD:
    CD274;
    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    Interacts with PDCD1.
    Function:
    Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.
    Subcellular Location:
    Isoform 2 Endomembrane system Single-pass type I membrane protein
    Protein Attributes:
    Sequence length:
    290
    Sequence:
    50:
    MRIFAVFIFM | TYWHLLNAFT | VTVPKDLYVV | EYGSNMTIEC | KFPVEKQLDL | 
    100:
    AALIVYWEME | DKNIIQFVHG | EEDLKVQHSS | YRQRARLLKD | QLSLGNAALQ | 
    150:
    ITDVKLQDAG | VYRCMISYGG | ADYKRITVKV | NAPYNKINQR | ILVVDPVTSE | 
    200:
    HELTCQAEGY | PKAEVIWTSS | DHQVLSGKTT | TTNSKREEKL | FNVTSTLRIN | 
    250:
    TTTNEIFYCT | FRRLDPEENH | TAELVIPELP | LAHPPNERTH | LVILGAILLC | 
    290:
    LGVALTFIFR | LRKGRMMDVK | KCGIQDTNSK | KQSDTHLEET | 
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    Pfam:
    SMR:
    String:
    KEGG:
    MIM:
    UniGene:
    Uniprot:
     
    FOR
    ELISA Kit for Human PD-L1
    Cat.:
    E0513h
    Price:
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    MSDS:
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    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human PD-L1
    Cat.:
    E0513m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human PD-L1
    Cat.:
    U0513h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human PD-L1
    Cat.:
    U0513m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Human PD-L1
    Cat.:
    P0513Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human PD-L1
    Cat.:
    P0513Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Human PD-L1
    Monoclonal Antibody for Human PD-L1
    Protein for Human PD-L1
    Protein for Human PD-L1

    R&D Technical Data
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Precision
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤4.7%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.1%

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Recovery
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

    Recovery was determined by spiking various levels of PD1L1 into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    104

    95-106

    Plasma

    102

    93-105

     

     

     

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Linearity
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PD1L1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    98-110%

    97-109%

    100-106%

    98-108%

    EDTA plasma(n=5)

    95-109%

    97-108%

    99-106%

    95-107%

    heparin plasma(n=5)

    96-106%

     

    94-109%

    98-107%

    97-106%

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    References
    1. 1.
      "B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin-10 secretion."
      Dong H. , Zhu G. , Tamada K. , Chen L.
      Nat. Med.5:1365-1369(1999) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);FUNCTION;TISSUE SPECIFICITY;INDUCTION
    2. 2.
      "Engagement of the PD-1 immunoinhibitory receptor by a novel B7-family member leads to negative regulation of lymphocyte activation."
      Freeman G.J. , Long A.J. , Iwai Y. , Bourque K. , Chernova T. , Nishimura H. , Fitz L.J. , Malenkovich N. , Okazaki T. , Byrne M.C. , Horton H.F. , Fouser L. , Carter L. , Ling V. , Bowman M.R. , Carreno B.M. , Collins M. , Wood C.R. , more...
      J. Exp. Med.192:1027-1034(2000) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);FUNCTION;INTERACTION WITH PDCD1;TISSUE SPECIFICITY;INDUCTION
      tissue: Placenta.
    3. 3.
      "Identification of a novel splice variant of human PD-L1 mRNA encoding an isoform-lacking Igv-like domain."
      He X.-H. , Xu L.-H. , Liu Y.
      Acta Pharmacol. Sin.26:462-468(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3);SUBCELLULAR LOCATION
    4. 4.
      "Expression and significance of PD-L1 variant within tissues of human gastric carcinoma."
      Chi X.-Y. , Zha Q.-B. , Xu L.-H. , Jia Q.-T. , Li F.-Y. , He X.-H.
      Submitted (2005-11) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2)
      tissue: Gastric carcinoma.
    5. 5.
      "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
      Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3)
      tissue: Placenta.
    6. 6.
      "DNA sequence and analysis of human chromosome 9."
      Humphray S.J. , Oliver K. , Hunt A.R. , Plumb R.W. , Loveland J.E. , Howe K.L. , Andrews T.D. , Searle S. , Hunt S.E. , Scott C.E. , Jones M.C. , Ainscough R. , Almeida J.P. , Ambrose K.D. , Ashwell R.I.S. , Babbage A.K. , Babbage S. , Bagguley C.L. , more...
      Nature429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    7. 7.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    8. 8.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
      tissue: Lung.
    9. 9.
      "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
      Chen R. , Jiang X. , Sun D. , Han G. , Wang F. , Ye M. , Wang L. , Zou H.
      J. Proteome Res.8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-192
      tissue: Liver.
    10. 10.
      "The PD-1/PD-L1 complex resembles the antigen-binding Fv domains of antibodies and T cell receptors."
      Lin D.Y. , Tanaka Y. , Iwasaki M. , Gittis A.G. , Su H.P. , Mikami B. , Okazaki T. , Honjo T. , Minato N. , Garboczi D.N.
      Proc. Natl. Acad. Sci. U.S.A.105:3011-3016(2008) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.64 ANGSTROMS) OF 18-239 IN COMPLEX WITH MOUSE PDCD1;DISULFIDE BONDS
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