PRF1 ELISA Kit for Human(Homo sapiens) | EIAab.comPRF1 ELISA Kit for Human(Homo sapiens)

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Index > Protein center > PRF1(Gene name) > Human

PRF1 (Gene name),

Perforin-1 (Protein name ), PERF_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

PRF1(PFP);

Protein name:

Perforin-1(P1);

Alternative:

Lymphocyte pore-forming protein(PFP);Cytolysin;

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Monomer, as sobluble protein. Homooligomer. Oligomerization is required for pore formation.

Function:

Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.

Subcellular Location:

Cytoplasmic granule lumen Secreted Cell membrane Multi-pass membrane protein Endosome lumen Stored in cytoplasmic granules of cytolytic T-lymphocytes and secreted into the cleft between T-lymphocyte and target cell. Inserts into the cell membrane of target cells and forms pores. Membrane insertion and pore formation requires a major conformation change. May be taken up via endocytosis involving clathrin-coated vesicles and accumulate in a first time in large early endosomes.

Protein Attributes:

Sequence length:

555

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

550:

555:

SGAVW

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

MIM:

170280

String:

P14222

UniGene:

Hs.2200

SMR:

P14222

KEGG:

hsa:5551

STRING:

9606.ENSP00000316746

Pfam:

PF00168

Uniprot:

P14222

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ELISA Kit for Human P1

Cat.:

E1317h

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MSDS:

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Packing:

96T

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ELISA Kit for Human P1

Cat.:

E1317r

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Packing:

96T

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ELISA Kit for Human P1

Cat.:

E1317m

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MSDS:

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Packing:

96T

CLIA Kit for Human P1

Cat.:

U1317r

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Manual:

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Packing:

96T

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CLIA Kit for Human P1

Cat.:

U1317h

Price:

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Manual:

Looking for manual.

MSDS:

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Packing:

96T

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CLIA Kit for Human P1

Cat.:

U1317m

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MSDS:

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Packing:

96T

Polyclonal Antibody for Human P1

Cat.:

P1317Rb-m

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human P1

Cat.:

P1317Rb-r

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human P1

Cat.:

P1317Rb-h

Price:

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Packing:

40ug/0.2ml

Monoclonal Antibody for Human P1

Protein for Human P1

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

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Linearity

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References

1.

"Structure and function of human perforin."
Lichtenheld M.G. , Olsen K.J. , Lu P. , Lowrey D.M. , Hameed A. , Hengartner H. , Podack E.R.
Nature335:448-451(1988) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
tissue: Natural killer cell.
2.

"Molecular cloning and chromosomal assignment of a human perforin (PFP) gene."
Shinkai Y. , Yoshida C.M. , Maeda K. , Kobata T. , Maruyama K. , Yodoi J. , Yagita H. , Okumura K.
Immunogenetics30:452-457(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
3.

"Structure of the human perforin gene. A simple gene organization with interesting potential regulatory sequences."
Lichtenheld M.G. , Podack E.R.
J. Immunol.143:4267-4274(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
4.

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
tissue: Spleen.
5.

"The DNA sequence and comparative analysis of human chromosome 10."
Deloukas P. , Earthrowl M.E. , Grafham D.V. , Rubenfield M. , French L. , Steward C.A. , Sims S.K. , Jones M.C. , Searle S. , Scott C. , Howe K. , Hunt S.E. , Andrews T.D. , Gilbert J.G.R. , Swarbreck D. , Ashurst J.L. , Taylor A. , Battles J. , more...
Nature429:375-381(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
6.

Mural R.J. , Istrail S. , Sutton G.G. , Florea L. , Halpern A.L. , Mobarry C.M. , Lippert R. , Walenz B. , Shatkay H. , Dew I. , Miller J.R. , Flanigan M.J. , Edwards N.J. , Bolanos R. , Fasulo D. , Halldorsson B.V. , Hannenhalli S. , Turner R. , more...
Submitted (2005-07) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
7.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
8.

Totoki Y. , Toyoda A. , Takeda T. , Sakaki Y. , Tanaka A. , Yokoyama S. , Ohara O. , Nagase T. , Kikuno R.F.
Submitted (2005-03) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 258-555
tissue: Spleen.
9.

"Target cell-induced perforin mRNA turnover in NK3.3 cells is mediated by multiple elements within the mRNA coding region."
Goebel W.S. , Schloemer R.H. , Brahmi Z.
Mol. Immunol.33:341-349(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 520-555;INDUCTION
tissue: Natural killer cell.
10.

"Human autoreactive CD4+ T cell clones use perforin- or Fas/Fas ligand-mediated pathways for target cell lysis."
Vergelli M. , Hemmer B. , Muraro P.A. , Tranquill L. , Biddison W.E. , Sarin A. , McFarland H.F. , Martin R.
J. Immunol.158:2756-2761(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
11.

"Perforin, Fas/Fas ligand, and TNF-alpha pathways as specific and bystander killing mechanisms of hepatitis C virus-specific human CTL."
Ando K. , Hiroishi K. , Kaneko T. , Moriyama T. , Muto Y. , Kayagaki N. , Yagita H. , Okumura K. , Imawari M.
J. Immunol.158:5283-5291(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
12.

"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
Chen R. , Jiang X. , Sun D. , Han G. , Wang F. , Ye M. , Wang L. , Zou H.
J. Proteome Res.8:651-661(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-205
tissue: Liver.
13.

"Perforin activates clathrin- and dynamin-dependent endocytosis, which is required for plasma membrane repair and delivery of granzyme B for granzyme-mediated apoptosis."
Thiery J. , Keefe D. , Saffarian S. , Martinvalet D. , Walch M. , Boucrot E. , Kirchhausen T. , Lieberman J.
Blood115:1582-1593(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;SUBCELLULAR LOCATION
14.

"Human perforin employs different avenues to damage membranes."
Praper T. , Sonnen A. , Viero G. , Kladnik A. , Froelich C.J. , Anderluh G. , Dalla Serra M. , Gilbert R.J.
J. Biol. Chem.286:2946-2955(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;CALCIUM-DEPENDENT PORE FORMING ACTIVITY;SUBCELLULAR LOCATION;SUBUNIT;ELECTRON MICROSCOPY
15.

"Reciprocal granzyme/perforin-mediated death of human regulatory and responder T cells is regulated by interleukin-2 (IL-2)."
Czystowska M. , Strauss L. , Bergmann C. , Szajnik M. , Rabinowich H. , Whiteside T.L.
J. Mol. Med.88:577-588(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
16.

"Localization and molecular modelling of the membrane-inserted domain of the ninth component of human complement and perforin."
Peitsch M.C. , Amiguet P. , Guy R. , Brunner J. , Maizel J.V. Jr. , Tschopp J.
Mol. Immunol.27:589-602(1990) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: 3D-STRUCTURE MODELING OF MEMBRANE-SPANNING DOMAIN (MSD)
17.

"The structural basis for membrane binding and pore formation by lymphocyte perforin."
Law R.H. , Lukoyanova N. , Voskoboinik I. , Caradoc-Davies T.T. , Baran K. , Dunstone M.A. , D'Angelo M.E. , Orlova E.V. , Coulibaly F. , Verschoor S. , Browne K.A. , Ciccone A. , Kuiper M.J. , Bird P.I. , Trapani J.A. , Saibil H.R. , Whisstock J.C.
Nature468:447-451(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ELECTRON MICROSCOPY OF PORE COMPLEX;FUNCTION;SUBCELLULAR LOCATION;SUBUNIT
18.

"Perforin gene defects in familial hemophagocytic lymphohistiocytosis."
Stepp S.E. , Dufourcq-Lagelouse R. , Le Deist F. , Bhawan S. , Certain S. , Mathew P.A. , Henter J.-I. , Bennett M. , Fischer A. , de Saint Basile G. , Kumar V.
Science286:1957-1959(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS FHL2 GLY-183; TRP-225; SER-252; TYR-279; LEU-345 AND GLU-429
19.

"Spectrum of perforin gene mutations in familial hemophagocytic lymphohistiocytosis."
Goeransdotter Ericson K. , Fadeel B. , Nilsson-Ardnor S. , Soederhaell C. , Samuelsson A. , Janka G. , Schneider M. , Guergey A. , Yalman N. , Revesz T. , Egeler R. , Jahnukainen K. , Storm-Mathiesen I. , Haraldsson A. , Poole J. , de Saint Basile G. , Nordenskjoeld M. , Henter J.-I.
Am. J. Hum. Genet.68:590-597(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS FHL2 MET-50; ASN-224 AND LYS-285 DEL
20.

"The consensus coding sequences of human breast and colorectal cancers."
Sjoeblom T. , Jones S. , Wood L.D. , Parsons D.W. , Lin J. , Barber T.D. , Mandelker D. , Leary R.J. , Ptak J. , Silliman N. , Szabo S. , Buckhaults P. , Farrell C. , Meeh P. , Markowitz S.D. , Willis J. , Dawson D. , Willson J.K.V. , more...
Science314:268-274(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT [LARGE SCALE ANALYSIS] HIS-123

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