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Index > Protein center > PSIP1(Gene name) > Human
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  • PSIP1 (Gene name),
  • PC4 and SFRS1-interacting protein (Protein name ),  PSIP1_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Gene name:
    PSIP1(DFS70;LEDGF;PSIP2);
    Protein name:
    PC4 and SFRS1-interacting protein;
    Alternative:
    Dense fine speckles 70 kDa protein(DFS 70);CLL-associated antigen KW-7;Transcriptional coactivator p75/p52;Lens epithelium-derived growth factor;
    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    Monomer. Interacts with IFRD1/PC4. Isoform 2 interacts with SFRS1. Isoform 1 interacts POGZ and CDCA7L. Isoform 1 interacts with lentiviral integrase and acts as a chromatin tethering factor to the chromosomal DNA. Isoform 1 interacts in particular with the human HIV-1 integrase protein (HIV-1 IN), determining its nuclear localization, its tight association with chromatin and its protection from the proteasome. Isoform 1 interacts also with HIV-2 IN. Isoform 2 does not interact with HIV-1 IN.
    Function:
    Transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. Involved in particular in lens epithelial cell gene regulation and stress responses. May play an important role in lens epithelial to fiber cell terminal differentiation. May play a protective role during stress-induced apoptosis. Isoform 2 is a more general and stronger transcriptional coactivator. Isoform 2 may also act as an adapter to coordinate pre-mRNA splicing. Cellular cofactor for lentiviral integration.
    Subcellular Location:
    Nucleus Remains chromatin-associated throughout the cell cycle.
    Protein Attributes:
    Sequence length:
    530
    Sequence:
    50:
    MTRDFKPGDL | IFAKMKGYPH | WPARVDEVPD | GAVKPPTNKL | PIFFFGTHET | 
    100:
    AFLGPKDIFP | YSENKEKYGK | PNKRKGFNEG | LWEIDNNPKV | KFSSQQAATK | 
    150:
    QSNASSDVEV | EEKETSVSKE | DTDHEEKASN | EDVTKAVDIT | TPKAARRGRK | 
    200:
    RKAEKQVETE | EAGVVTTATA | SVNLKVSPKR | GRPAATEVKI | PKPRGRPKMV | 
    250:
    KQPCPSESDI | ITEEDKSKKK | GQEEKQPKKQ | PKKDEEGQKE | EDKPRKEPDK | 
    300:
    KEGKKEVESK | RKNLAKTGVT | STSDSEEEGD | DQEGEKKRKG | GRNFQTAHRR | 
    350:
    NMLKGQHEKE | AADRKRKQEE | QMETEQQNKD | EGKKPEVKKV | EKKRETSMDS | 
    400:
    RLQRIHAEIK | NSLKIDNLDV | NRCIEALDEL | ASLQVTMQQA | QKHTEMITTL | 
    450:
    KKIRRFKVSQ | VIMEKSTMLY | NKFKNMFLVG | EGDSVITQVL | NKSLAEQRQH | 
    500:
    EEANKTKDQG | KKGPNKKLEK | EQTGSKTLNG | GSDAQDGNQP | QHNGESNEDS | 
    530:
    KDNHEASTKK | KPSSEERETE | ISLKDSTLDN | 
    3D Structure:
    N/A
    Predicted Eptitope:
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    EIAab Sequence  Vaild Sequence:
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    Related Databases
    KEGG:
    Pfam:
    UniGene:
    String:
    MIM:
    UniGene:
    SMR:
    Uniprot:
     
    FOR
    ELISA Kit for Human PC4 and SFRS1-interacting protein
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    E15031h
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    ELISA Kit for Human PC4 and SFRS1-interacting protein
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    E15031b
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    ELISA Kit for Human PC4 and SFRS1-interacting protein
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    ELISA Kit for Human PC4 and SFRS1-interacting protein
    Cat.:
    E15031m
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    ELISA Kit for Human PC4 and SFRS1-interacting protein
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    E15031r
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    MSDS:
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    96T
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    CLIA Kit for Human PC4 and SFRS1-interacting protein
    MSDS:
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    CLIA Kit for Human PC4 and SFRS1-interacting protein
    Cat.:
    U15031h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human PC4 and SFRS1-interacting protein
    MSDS:
    Please sign in first.
    CLIA Kit for Human PC4 and SFRS1-interacting protein
    MSDS:
    Please sign in first.
    CLIA Kit for Human PC4 and SFRS1-interacting protein
    MSDS:
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    Polyclonal Antibody for Human PC4 and SFRS1-interacting protein
    Polyclonal Antibody for Human PC4 and SFRS1-interacting protein
    Polyclonal Antibody for Human PC4 and SFRS1-interacting protein
    Polyclonal Antibody for Human PC4 and SFRS1-interacting protein
    Polyclonal Antibody for Human PC4 and SFRS1-interacting protein
    Monoclonal Antibody for Human PC4 and SFRS1-interacting protein
    Monoclonal Antibody for Human PC4 and SFRS1-interacting protein
    Monoclonal Antibody for Human PC4 and SFRS1-interacting protein
    Monoclonal Antibody for Human PC4 and SFRS1-interacting protein
    Monoclonal Antibody for Human PC4 and SFRS1-interacting protein
    Protein for Human PC4 and SFRS1-interacting protein
    Protein for Human PC4 and SFRS1-interacting protein
    Protein for Human PC4 and SFRS1-interacting protein
    Protein for Human PC4 and SFRS1-interacting protein
    Protein for Human PC4 and SFRS1-interacting protein

    R&D Technical Data
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    Precision
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    Linearity
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    References
    1. 1.
      "Isolation of cDNAs encoding novel transcription coactivators p52 and p75 reveals an alternate regulatory mechanism of transcriptional activation."
      Ge H. , Si Y. , Roeder R.G.
      EMBO J.17:6723-6729(1998) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2);PROTEIN SEQUENCE OF 4-39 AND 76-89;TISSUE SPECIFICITY;INTERACTION WITH PC4
      tissue: Cervix carcinoma.
    2. 2.
      "Lens epithelium-derived growth factor: effects on growth and survival of lens epithelial cells, keratinocytes, and fibroblasts."
      Singh D.P. , Ohguro N. , Kikuchi T. , Sueno T. , Reddy V.N. , Yuge K. , Chylack L.T. Jr. , Shinohara T.
      Biochem. Biophys. Res. Commun.267:373-381(2000) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
      tissue: Lens.
    3. 3.
      "Lens epithelium-derived growth factor (LEDGF/p75) and p52 are derived from a single gene by alternative splicing."
      Singh D.P. , Kimura A. , Chylack L.T. Jr. , Shinohara T.
      Gene242:265-273(2000) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1 AND 2)
    4. 4.
      "Identification of tumor-associated antigens in chronic lymphocytic leukemia by SEREX."
      Krackhardt A.M. , Witzens M. , Harig S. , Hodi F.S. , Zauls A.J. , Chessia M. , Barrett P. , Gribben J.G.
      Blood100:2123-2131(2002) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
      tissue: Leukemia.
    5. 5.
      "DNA sequence and analysis of human chromosome 9."
      Humphray S.J. , Oliver K. , Hunt A.R. , Plumb R.W. , Loveland J.E. , Howe K.L. , Andrews T.D. , Searle S. , Hunt S.E. , Scott C.E. , Jones M.C. , Ainscough R. , Almeida J.P. , Ambrose K.D. , Ashwell R.I.S. , Babbage A.K. , Babbage S. , Bagguley C.L. , more...
      Nature429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    6. 6.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    7. 7.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3)
      tissue: Testis.
      tissue: Uterus.
    8. 8.
      "Autoantibodies to DFS 70 kd/transcription coactivator p75 in atopic dermatitis and other conditions."
      Ochs R.L. , Muro Y. , Si Y. , Ge H. , Chan E.K.L. , Tan E.M.
      J. Allergy Clin. Immunol.105:1211-1220(2000) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 180-530 (ISOFORM 1)
    9. 9.
      "Lens epithelium-derived growth factor (LEDGF/p75) expression in fetal and adult human brain."
      Chylack L.T. Jr. , Fu L. , Mancini R. , Martin-Rehrmann M.D. , Saunders A.J. , Konopka G. , Tian D. , Hedley-Whyte E.T. , Folkerth R.D. , Goldstein L.E.
      Exp. Eye Res.79:941-948(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;TISSUE SPECIFICITY;SUBCELLULAR LOCATION
    10. 10.
      "Lens epithelium-derived growth factor/p75 prevents proteasomal degradation of HIV-1 integrase."
      Llano M. , Delgado S. , Vanegas M. , Poeschla E.M.
      J. Biol. Chem.279:55570-55577(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH HUMAN HIV-1 INTEGRASE (ISOFORM 1)
    11. 11.
      "The interaction of LEDGF/p75 with integrase is lentiviral-specific and promotes DNA binding."
      Busschots K. , Vercammen J. , Emiliani S. , Benarous R. , Engelborghs Y. , Christ F. , Debyser Z.
      J. Biol. Chem.280:17841-17847(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH HUMAN HIV-1 AND HIV-2 INTEGRASE (ISOFORM 1)
    12. 12.
      "Identification of the LEDGF/p75 HIV-1 integrase-interaction domain and NLS reveals NLS-independent chromatin tethering."
      Vanegas M. , Llano M. , Delgado S. , Thompson D. , Peretz M. , Poeschla E.M.
      J. Cell Sci.118:1733-1743(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH HUMAN HIV-1 INTEGRASE (ISOFORM 1);NUCLEAR LOCALIZATION SIGNAL
    13. 13.
      "A novel transcriptional coactivator, p52, functionally interacts with the essential splicing factor ASF/SF2."
      Ge H. , Si Y. , Wolffe A.P.
      Mol. Cell2:751-759(1998) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH SFRS1;SUBCELLULAR LOCATION
    14. 14.
      "t(9;11)(p22;p15) with NUP98-LEDGF fusion gene in pediatric acute myeloid leukemia."
      Morerio C. , Acquila M. , Rosanda C. , Rapella A. , Tassano E. , Micalizzi C. , Panarello C.
      Leuk. Res.29:467-470(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: CHROMOSOMAL TRANSLOCATION WITH NUP98
    15. 15.
      "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
      Olsen J.V. , Blagoev B. , Gnad F. , Macek B. , Kumar C. , Mortensen P. , Mann M.
      Cell127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-122;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Cervix carcinoma.
    16. 16.
      "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."
      Matsuoka S. , Ballif B.A. , Smogorzewska A. , McDonald E.R. III , Hurov K.E. , Luo J. , Bakalarski C.E. , Zhao Z. , Solimini N. , Lerenthal Y. , Shiloh Y. , Gygi S.P. , Elledge S.J.
      Science316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-437;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Embryonic kidney.
    17. 17.
      "Phosphorylation analysis of primary human T lymphocytes using sequential IMAC and titanium oxide enrichment."
      Carrascal M. , Ovelleiro D. , Casas V. , Gay M. , Abian J.
      J. Proteome Res.7:5167-5176(2008) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: T-cell.
    18. 18.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-106; THR-141; SER-273; SER-275; SER-434; SER-443; SER-522 AND THR-527;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Cervix carcinoma.
    19. 19.
      "Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography."
      Han G. , Ye M. , Zhou H. , Jiang X. , Feng S. , Jiang X. , Tian R. , Wan D. , Zou H. , Gu J.
      Proteomics8:1346-1361(2008) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Liver.
    20. 20.
      "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S. , Helbig A.O. , Slijper M. , Krijgsveld J. , Heck A.J. , Mohammed S.
      Anal. Chem.81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
    21. 21.
      "Lens epithelium-derived growth factor/p75 interacts with the transposase-derived DDE domain of PogZ."
      Bartholomeeusen K. , Christ F. , Hendrix J. , Rain J.C. , Emiliani S. , Benarous R. , Debyser Z. , Gijsbers R. , De Rijck J.
      J. Biol. Chem.284:11467-11477(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH POGZ; HIV-1 INTEGRASE AND CDCA7L;MUTAGENESIS OF LYS-360; ILE-365; ASP-366; VAL-370; PHE-406 AND VAL-408
    22. 22.
      "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V. , Lundgren D.H. , Hwang S.-I. , Rezaul K. , Wu L. , Eng J.K. , Rodionov V. , Han D.K.
      Sci. Signal.2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-141; THR-167; SER-177; SER-273 AND SER-275;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Leukemic T-cell.
    23. 23.
      "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V. , Vermeulen M. , Santamaria A. , Kumar C. , Miller M.L. , Jensen L.J. , Gnad F. , Cox J. , Jensen T.S. , Nigg E.A. , Brunak S. , Mann M.
      Sci. Signal.3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-106; THR-122; THR-141; SER-206; SER-273; SER-275; SER-434 AND THR-527;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Cervix carcinoma.
    24. 24.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
    25. 25.
      "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T. , Prokhorova T.A. , Akimov V. , Henningsen J. , Johansen P.T. , Kratchmarova I. , Kassem M. , Mann M. , Olsen J.V. , Blagoev B.
      Sci. Signal.4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-102; SER-106; SER-129; THR-141; SER-177; SER-206; SER-275 AND THR-527;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
    26. 26.
      "Solution structure of the HIV-1 integrase-binding domain in LEDGF/p75."
      Cherepanov P. , Sun Z.-Y. , Rahman S. , Maertens G. , Wagner G. , Engelman A.
      Nat. Struct. Mol. Biol.12:526-532(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 347-471;SUBUNIT;MUTAGENESIS OF ILE-365; ASP-366; PHE-406 AND VAL-408
    27. 27.
      "Structural basis for the recognition between HIV-1 integrase and transcriptional coactivator p75."
      Cherepanov P. , Ambrosio A.L.B. , Rahman S. , Ellenberger T. , Engelman A.
      Proc. Natl. Acad. Sci. U.S.A.102:17308-17313(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.02 ANGSTROMS) OF 347-442 IN COMPLEX WITH HUMAN HIV-1 INTEGRASE
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