Tnf Protein for Mouse(Mus musculus) | EIAab.comTnf Protein for Mouse(Mus musculus)

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Index > Protein center > Tnf(Gene name) > Mouse

Tnf (Gene name),

Tumor necrosis factor (Protein name ), TNFA_MOUSE from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

Citations (12)

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Gene name:

Tnf(Tnfa;Tnfsf2);

Protein name:

Tumor necrosis factor;

Alternative:

TNF-alpha;Cachectin;Tumor necrosis factor ligand superfamily member 2(TNF-a);

Organism:

Mouse (Mus musculus).

General Annotation

Sub Unit:

Homotrimer.

Function:

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.

Subcellular Location:

Tumor necrosis factor, soluble form Secreted

Protein Attributes:

Sequence length:

235

Sequence:

50:

100:

150:

200:

235:

VFQLEKGDQL | SAEVNLPKYL | DFAESGQVYF | GVIAL

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

Pfam:

PF00229

UniGene:

Mm.1293

KEGG:

mmu:21926

String:

P06804

SMR:

P06804

Uniprot:

P06804

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ELISA Kit for Mouse Tumor necrosis factor

Cat.:

E0133h

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96T

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ELISA Kit for Mouse Tumor necrosis factor

Cat.:

E0133d

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96T

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ELISA Kit for Mouse Tumor necrosis factor

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E0133p

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96T

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ELISA Kit for Mouse Tumor necrosis factor

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ELISA Kit for Mouse Tumor necrosis factor

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E0133m

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96T

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ELISA Kit for Mouse Tumor necrosis factor

Cat.:

E0133r

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96T

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ELISA Kit for Mouse Tumor necrosis factor

Cat.:

E0133b

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96T

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CLIA Kit for Mouse Tumor necrosis factor

Cat.:

U0133b

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96T

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CLIA Kit for Mouse Tumor necrosis factor

Cat.:

U0133p

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96T

CLIA Kit for Mouse Tumor necrosis factor

Cat.:

U0133m

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96T

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CLIA Kit for Mouse Tumor necrosis factor

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CLIA Kit for Mouse Tumor necrosis factor

Cat.:

U0133h

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96T

CLIA Kit for Mouse Tumor necrosis factor

Cat.:

U0133d

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96T

CLIA Kit for Mouse Tumor necrosis factor

Cat.:

U0133r

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96T

Polyclonal Antibody for Mouse Tumor necrosis factor

Cat.:

P0133Rb-m

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Packing:

40ug/0.2ml

Polyclonal Antibody for Mouse Tumor necrosis factor

Cat.:

P0133Rb-h

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40ug/0.2ml

Polyclonal Antibody for Mouse Tumor necrosis factor

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P0133Rb-r

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40ug/0.2ml

Monoclonal Antibody for Mouse Tumor necrosis factor

Protein for Mouse Tumor necrosis factor

Cat.:

R0133h

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Packing:

1mg

Purity:

>90%

Protein for Mouse Tumor necrosis factor

Cat.:

R0133m

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1mg

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>90%

Protein for Mouse Tumor necrosis factor

Cat.:

R0133r

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1mg

Purity:

>90%

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.2%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.4%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.0%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of TNF into serum and plasma .

Sample Type	Average(%)	Recovery Range (%)
Serum	102	95-114
Plasma	96	93-99

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery was determined by spiking various levels of TNF into serum and plasma .

Sample Type	Average(%)	Recovery Range (%)
Serum	89	86-104
Plasma	95	86-105

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	96-101%	96-105%	98-104%	98-107%
EDTA plasma(n=5)	95-103%	97-107%	100-110%	88-103%
heparin plasma(n=5)	98-103%	96-102%	93-104%	95-115%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-103%	90-101%	98-109%	97-110%
EDTA plasma(n=5)	101-105%	97-109%	89-107%	99-108%
heparin plasma(n=5)	98-107%	96-110%	100-112%	101-116%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Citations

[1].

"β-Lapachone protects against doxorubicin-induced nephrotoxicity via NAD+/AMPK/NF-kB in mice"

D Sanajou, V Hosseini, Y Marandi, et al.

From:Naunyn-Schmiedeberg Archives of Pharmacology

[2].

"Propofol Inhibits the Activation of p38 through Up-Regulating the Expression of Annexin A1 to Exert Its Anti-Inflammation Effect"

Jing Tang, Xi Chen, Weifeng Tu, Yuanbo Guo, Zhenlong Zhao, Qiong Xue, Chunshui Lin, Jinfang Xiao, Xuegang Sun, Tao Tao, Miaoning Gu, Youtan Liu

From:Plos ONE

[3].

"Simvastatin Attenuates Intestinal Fibrosis Independent of the Anti-Inflammatory Effect by Promoting Fibroblast/Myofibroblast Apoptosis"

Y Abe, M Murano, N Murano, et al.

From:Digestive diseases and sciences

[4].

"Staphylococcus aureus supernatant induces the release of mouse β-defensin-14 from osteoblasts via the p38 MAPK and NF-κB pathways"

Chen Zhu, Hui Qin, Tao Cheng, Hong-Lue Tan, Yong-Yuan Guo, Si-Feng Shi, De-Sheng Chen, Xian-Long Zhang

From:Int J Mol Med

[5].

"MicroRNA-21 Knockout Improve the Survival Rate in DSS Induced Fatal Colitis through Protecting against Inflammation and Tissue Injury"

C Shi, Y Liang, J Yang, et al.

From:PloS one

[6].

"Retinal structure and function preservation by polysaccharides of wolfberry in a mouse model of retinal degeneration"

Ke Wang, Jia Xiao, Bo Peng, et al.

From:Scientific Reports

[7].

"Neonatal Bacillus Calmette-Guérin vaccination alleviates lipopolysaccharide-induced neurobehavioral impairments and neuroinflammation in adult mice"

Yang J, Qi F, Yao Z.

From:Molecular Medicine Reports

[8].

"Exploration of drug therapy in mouse models of human retinal diseases"

Wang, Ke

From:biomedical sciences

[9].

"In vitro morphology, viability and cytokine secretion of uterine telocyte-activated mouse peritoneal macrophages"

Chi C, Jiang X J, Su L, et al.

From:Journal of cellular and molecular medicine

[10].

"ACF7 regulates inflammatory colitis and intestinal wound response by orchestrating tight junction dynamics"

Yanlei M, Jiping Y, Chenzhang S, et al.

From:Nature communications

[11].

"Tetramethylpyrazine (TMP) protects rats against acute pancreatitis through NF-κB pathway"

Ly Chen, Yj Chen, H Yun, et al.

From:Bioengineered

[12].

"Carvedilol protects against hepatic ischemia reperfusion injury in high-fructose high-fat diet-fed mice: Role of G protein-coupled receptor kinase 2 a"

S G. Mohammed, A A.A.Mahmoud, M F. Mahmoud, et al.

From:Toxicology and Applied Pharmacology

References

1.

"Cloning and expression in Escherichia coli of the gene for mouse tumor necrosis factor."
Shirai T. , Shimizu N. , Shiojiri S. , Horiguchi S. , Ito H.
DNA7:193-201(1988) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
2.

"Cloning and expression in Escherichia coli of the cDNA for murine tumor necrosis factor."
Pennica D. , Hayflick J.S. , Bringman T.S. , Palladino M.A. , Goeddel D.V.
Proc. Natl. Acad. Sci. U.S.A.82:6060-6064(1985) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
3.

"Identification of a common nucleotide sequence in the 3'-untranslated region of mRNA molecules specifying inflammatory mediators."
Caput D. , Beutler B. , Hartog K. , Thayer R. , Brown-Shimer S. , Cerami A.
Proc. Natl. Acad. Sci. U.S.A.83:1670-1674(1986) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
4.

"Molecular cloning of mouse tumour necrosis factor cDNA and its eukaryotic expression."
Fransen L. , Mueller R. , Marmenout A. , Tavernier J. , van der Heyden J. , Kawashima E. , Chollet A. , Tizard R. , van Heuverswyn H. , van Vliet A. , Ruysschaert M.-R. , Fiers W.
Nucleic Acids Res.13:4417-4429(1985) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
5.

"Molecular cloning of genes coding for tumor necrosis factor. Complete nucleotide sequence of the genome copy of TNF-alpha in mice."
Shakhov A.N. , Nedospasov S.A.
Bioorg. Khim.13:701-705(1987) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE
6.

"Nucleotide sequence of the murine TNF locus, including the TNF-alpha (tumor necrosis factor) and TNF-beta (lymphotoxin) genes."
Semon D. , Kawashima E. , Jongeneel C.V. , Shakhov A.N. , Nedospasov S.A.
Nucleic Acids Res.15:9083-9084(1987) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
7.

"Identification of a new susceptibility locus for insulin-dependent diabetes mellitus by ancestral haplotype congenic mapping."
Ikegami H. , Makino S. , Yamato E. , Kawaguchi Y. , Ueda H. , Sakamoto T. , Takekawa K. , Ogihara T.
J. Clin. Invest.96:1936-1942(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
strain: CTS.
strain: NOD.
8.

"Cloning and sequencing of the Tnfa genes of three inbred mouse strains."
Iraqi F. , Teale A.
Immunogenetics45:459-461(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANTS THR-7 AND ALA-77
strain: A/J.
strain: BALB/c.
strain: C57BL/6.
9.

"Analysis of the gene-dense major histocompatibility complex class III region and its comparison to mouse."
Xie T. , Rowen L. , Aguado B. , Ahearn M.E. , Madan A. , Qin S. , Campbell R.D. , Hood L.
Genome Res.13:2621-2636(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
strain: 129.
10.

"Conspicuous differences among gene genealogies of 21 nuclear genes of five Mus musculus subspecies."
Liu Y. , Kitano T. , Koide T. , Shiroishi T. , Moriwaki K. , Saitou N.
Submitted (2000-02) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE OF 1-96
strain: BFM/2Msf.
strain: BLG2/Msf.
strain: C57BL/10SnJ.
strain: CAST/Ei.
strain: HMI/Msf.
strain: MSM/Msf.
strain: NJL/Msf.
strain: pgn2.
strain: SWN/Msf.
11.

"Alternative cleavage of the cachectin/tumor necrosis factor propeptide results in a larger, inactive form of secreted protein."
Cseh K. , Beutler B.
J. Biol. Chem.264:16256-16260(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 70-87
12.

"Characterization of high molecular weight glycosylated forms of murine tumor necrosis factor."
Sherry B. , Juc D.-M. , Zentella A. , Cerami A.
Biochem. Biophys. Res. Commun.173:1072-1078(1990) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 80-99
13.

"A novel form of TNF/cachectin is a cell surface cytotoxic transmembrane protein: ramifications for the complex physiology of TNF."
Kriegler M. , Perez X. , Defay K. , Albert I. , Lu S.D.
Cell53:45-53(1988) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION OF MEMBRANE-BOUND FORM
14.

"The structure of mouse tumour-necrosis factor at 1.4 A resolution: towards modulation of its selectivity and trimerization."
Baeyens K.J. , De Bondt H.L. , Raeymaekers A. , Fiers W. , De Ranter C.J.
Acta Crystallogr. D55:772-778(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.4 ANGSTROMS) OF 80-235

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