Tnf Protein for Rat(Rattus norvegicus) | EIAab.comTnf Protein for Rat(Rattus norvegicus)

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Tnf (Gene name),

Tumor necrosis factor (Protein name ), TNFA_RAT from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

Citations (16)

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Gene name:

Tnf(Tnfa;Tnfsf2);

Protein name:

Tumor necrosis factor;

Alternative:

TNF-alpha;Cachectin;Tumor necrosis factor ligand superfamily member 2(TNF-a);

Organism:

Rat (Rattus norvegicus).

General Annotation

Sub Unit:

Homotrimer.

Function:

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.

Subcellular Location:

Tumor necrosis factor, soluble form Secreted

Protein Attributes:

Sequence length:

235

Sequence:

50:

100:

150:

200:

235:

VFQLEKGDLL | SAEVNLPKYL | DITESGQVYF | GVIAL

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

Pfam:

PF00229

KEGG:

rno:24835

UniGene:

Rn.2275

SMR:

P16599

String:

P16599

Uniprot:

P16599

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ELISA Kit for Rat Tumor necrosis factor

Cat.:

E0133h

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Packing:

96T

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ELISA Kit for Rat Tumor necrosis factor

Cat.:

E0133d

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96T

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ELISA Kit for Rat Tumor necrosis factor

Cat.:

E0133p

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96T

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ELISA Kit for Rat Tumor necrosis factor

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ELISA Kit for Rat Tumor necrosis factor

Cat.:

E0133m

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96T

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ELISA Kit for Rat Tumor necrosis factor

Cat.:

E0133r

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96T

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ELISA Kit for Rat Tumor necrosis factor

Cat.:

E0133b

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96T

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CLIA Kit for Rat Tumor necrosis factor

Cat.:

U0133b

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Looking for manual.

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Packing:

96T

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CLIA Kit for Rat Tumor necrosis factor

Cat.:

U0133p

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Packing:

96T

CLIA Kit for Rat Tumor necrosis factor

Cat.:

U0133m

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Looking for manual.

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Packing:

96T

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CLIA Kit for Rat Tumor necrosis factor

MSDS:

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CLIA Kit for Rat Tumor necrosis factor

Cat.:

U0133h

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Packing:

96T

CLIA Kit for Rat Tumor necrosis factor

Cat.:

U0133d

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96T

CLIA Kit for Rat Tumor necrosis factor

Cat.:

U0133r

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96T

Polyclonal Antibody for Rat Tumor necrosis factor

Cat.:

P0133Rb-m

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Packing:

40ug/0.2ml

Polyclonal Antibody for Rat Tumor necrosis factor

Cat.:

P0133Rb-h

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Packing:

40ug/0.2ml

Polyclonal Antibody for Rat Tumor necrosis factor

Cat.:

P0133Rb-r

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40ug/0.2ml

Monoclonal Antibody for Rat Tumor necrosis factor

Protein for Rat Tumor necrosis factor

Cat.:

R0133h

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Packing:

1mg

Purity:

>90%

Protein for Rat Tumor necrosis factor

Cat.:

R0133m

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Packing:

1mg

Purity:

>90%

Protein for Rat Tumor necrosis factor

Cat.:

R0133r

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Packing:

1mg

Purity:

>90%

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.2%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.4%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.0%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of TNF into serum and plasma .

Sample Type	Average(%)	Recovery Range (%)
Serum	102	95-114
Plasma	96	93-99

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery was determined by spiking various levels of TNF into serum and plasma .

Sample Type	Average(%)	Recovery Range (%)
Serum	89	86-104
Plasma	95	86-105

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	96-101%	96-105%	98-104%	98-107%
EDTA plasma(n=5)	95-103%	97-107%	100-110%	88-103%
heparin plasma(n=5)	98-103%	96-102%	93-104%	95-115%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-103%	90-101%	98-109%	97-110%
EDTA plasma(n=5)	101-105%	97-109%	89-107%	99-108%
heparin plasma(n=5)	98-107%	96-110%	100-112%	101-116%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Citations

[1].

"Protective effects of heme oxygenase-1 against severe acute pancreatitis via inhibition of tumor necrosis factor-α and augmentation of interleukin-10"

FH Zhang, YH Sun, KL Fan, et al.

From:BMC Gastroenterology

[2].

"Therapeutic effectiveness of rectally administered fish oil and mesalazine in trinitrobenzenesulfonic acid-induced colitis"

E Yorulmaz, H Yorulmaz, O Zengi, et al.

From:Biomedicine & Pharmacotherapy

[3].

"Effect of ω-3 Fatty Acid on GastrointestinalMotility after Abdominal Operation in Rats"

Q Zhang, JC Yu, WM Kang, et al.

From:Mediators of Inflammation

[4].

"Propofol Inhibits the Activation of p38 through Up-Regulating the Expression of Annexin A1 to Exert Its Anti-Inflammation Effect"

Jing Tang, Xi Chen, Weifeng Tu, Yuanbo Guo, Zhenlong Zhao, Qiong Xue, Chunshui Lin, Jinfang Xiao, Xuegang Sun, Tao Tao, Miaoning Gu, Youtan Liu

From:Plos ONE

[5].

"Alleviating effects of morin against experimentally-induced diabetic osteopenia"

Hatem M Abuohashish,Salim S Al-Rejaie ,Khaled A Al-Hosaini,Mihir Y Parmar,Mohammed M Ahmed

From:Diabetology & Metabolic Syndrome

[6].

"Involvement of K+ channel-dependant pathways in lipoxin A4-induced protective effects on hypoxia/reoxygenation injury of cardiomyocytes"

Xiao-Qing Chen, Sheng-Hua Wu, Yu Zhou, Yan-Rong Tang

From:Prostaglandins Leukot Essent Fatty Acids

[7].

"Flavonoid, morin inhibits oxidative stress, inflammation and enhances neurotrophic support in the brain of streptozotocin-induced diabetic rats"

MS Ola, AM Aleisa, SS Al-Rejaie, et al.

From:Neurological Sciences

[8].

"Involvement of K< sup>+ channel-dependant pathways in lipoxin A< sub> 4-induced protective effects on hypoxia/reoxygenation injury of card"

XQ Chen, SH Wu, Y Zhou, et al.

From:Prostaglandins, Leukotrienes and Essential Fatty Acids (PLEFA)

[9].

"Cathelicidin rCRAMP stimulates rat mast cells to cysteinyl leukotriene generation, TNF synthesis and migration. Involvement of PLC/A2, PI3K, and MAPK signaling pathways"

E Bąbolewska, A Pietrzak, E Brzezińska-Blaszczyk.

From:International immunology

[10].

"Transgenic flax overexpressing polyphenols as a potential anti-inflammatory dietary agent"

M Matusiewicz, I Kosieradzka, M Sobczak-Filipiak, et al.

From:Journal of Functional Foods

[11].

"Protective Effects of Phosphocreatine Administered Post-Treatment Combined With Ischemic Post-Conditioning on Rat Hearts With Myocardial Ischemia..."

W Zhang, H Zhang, Y Xing

From:Journal of clinical medicine research

[12].

"Protective Effects of Colchicine in an Experimental Rat Endometriosis Model Histopathological Evaluation and Assessment of TNF-α Levels"

RK Kurt, N Pinar, A Karateke, et al.

From:Reproductive Sciences

[13].

"Protective effects of rosuvastatin in a rat model of lung contusion: Stimulation of the cyclooxygenase 2–prostaglandin E-2 pathway"

O Dolkart, E Amar, S Shapira, et al.

From:Surgery

[14].

"Efficacy of coenzyme Q10 in mitigating spinal cord injury‑induced osteoporosis"

Zhang X X, Qian K J, Zhang Y, et al.

From:Molecular medicine reports

[15].

"Paeoniflorin ameliorates acute myocardial infarction of rats by inhibiting inflammation and inducible nitric oxide synthase signaling pathways"

Chen C, Du P, Wang J.

From:Molecular medicine reports

[16].

"A biodegradable multifunctional porous microsphere composed of carrageenan for promoting imageable trans-arterial chemoembolization"

KL Liu, ZC Jin, XL Hu, et al.

From:International Journal of Biological Macromolecules

References

1.

"Cloning and expression in Escherichia coli of the gene for rat tumor necrosis factor."
Shirai T. , Shimizu N. , Horiguchi S. , Ito H.
Agric. Biol. Chem.53:1733-1736(1989)

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
2.

"Rat tumor necrosis factor-alpha. Transcription in rat Kupffer cells and in vitro posttranslational processing based on a PCR-derived cDNA."
Estler H.C. , Grewe M. , Gaussling R. , Pavlovic M. , Decker K.F.
Biol. Chem. Hoppe-Seyler373:271-281(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
3.

"Cloning and sequence analysis of the rat tumor necrosis factor-encoding genes."
Kwon J. , Chung I.Y. , Benveniste E.N.
Gene132:227-236(1993) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
strain: Sprague-Dawley.
tissue: Testis.
4.

"Polymorphisms of the tumor necrosis factor alpha locus among autoimmune disease susceptible and resistant inbred rat strains."
Furuya T. , Joe B. , Salstrom J.L. , Hashiramoto A. , Dobbins D.E. , Wilder R.L. , Remmers E.F.
Genes Immun.2:229-232(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
strain: ACI/SegHsd.
strain: BB(DR)/Wor.
strain: Brown Norway/SsNHsd.
strain: DA/Bkl.
strain: F344/NHsd.
strain: LEW/NHsd.
5.

"TNF-alpha polymorphism in rats with collagen-induced arthritis."
Seidel M.F. , Junier M.-P. , Vetter H.
Submitted (2000-05) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];VARIANTS PRO-122 AND GLU-190
strain: Dark agouti.
6.

"No association between tumor necrosis factor-alpha production and gene polymorphisms among inbred rat strains."
Warle M.C. , van der Laan L.J. , Kusters J.G. , Pot R.G. , Hop W.C. , Segeren K.C. , Ijzermans J.N. , Metselaar H.J. , Tilanus H.W.
Transpl. Immunol.14:77-82(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
strain: AUG/OlaHsd.
strain: PVG/OlaHsd.
strain: WF/HanHsd.
7.

"The genomic sequence and comparative analysis of the rat major histocompatibility complex."
Hurt P. , Walter L. , Sudbrak R. , Klages S. , Mueller I. , Shiina T. , Inoko H. , Lehrach H. , Guenther E. , Reinhardt R. , Himmelbauer H.
Genome Res.14:631-639(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
strain: Brown Norway.
8.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
tissue: Prostate.
9.

"Mapping of the TNF-alpha locus in the rat."
Kirisits M.J. , Vardimon D. , Kunz H.W. , Gill T.J. III
Submitted (1993-06) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-231
tissue: Tail.

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