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Index > Protein center > RHOA(Gene name) > Human
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  • RHOA (Gene name),
  • Transforming protein RhoA (Protein name ),  RHOA_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Gene name:
    RHOA(ARH12;ARHA;RHO12);
    Protein name:
    Transforming protein RhoA;
    Alternative:
    Rho cDNA clone 12(h12);
    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    Interacts with RGNEF (By similarity). Binds PRKCL1, ROCK1 and ROCK2. Interacts with ARHGEF2, ARHGEF3, NET1 and RTKN. Interacts with PLCE1 and AKAP13. Interacts (in the constitutively activated, GTP-bound form) with DGKQ. Interacts with human respiratory syncytial virus (HRSV) protein F; this interaction facilitates virus-induced syncytium formation. Interacts with GNB2L1/RACK1; enhances RHOA activation.
    Function:
    Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
    Subcellular Location:
    Cell membrane Lipid-anchor Cytoplasmic side Cytoplasm cytoskeleton
    Protein Attributes:
    Sequence length:
    193
    Sequence:
    50:
    MAAIRKKLVI | VGDGACGKTC | LLIVFSKDQF | PEVYVPTVFE | NYVADIEVDG | 
    100:
    KQVELALWDT | AGQEDYDRLR | PLSYPDTDVI | LMCFSIDSPD | SLENIPEKWT | 
    150:
    PEVKHFCPNV | PIILVGNKKD | LRNDEHTRRE | LAKMKQEPVK | PEEGRDMANR | 
    193:
    IGAFGYMECS | AKTKDGVREV | FEMATRAALQ | ARRGKKKSGC | LVL
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    UniGene:
    String:
    KEGG:
    Pfam:
    SMR:
    MIM:
    Uniprot:
     
    FOR
    ELISA Kit for Human Transforming protein RhoA
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    E9513h
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    ELISA Kit for Human Transforming protein RhoA
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    ELISA Kit for Human Transforming protein RhoA
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    E9513b
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    ELISA Kit for Human Transforming protein RhoA
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    E9513m
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    ELISA Kit for Human Transforming protein RhoA
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    E9513r
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    CLIA Kit for Human Transforming protein RhoA
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    CLIA Kit for Human Transforming protein RhoA
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    U9513m
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    CLIA Kit for Human Transforming protein RhoA
    Cat.:
    U9513r
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    CLIA Kit for Human Transforming protein RhoA
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    U9513b
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    CLIA Kit for Human Transforming protein RhoA
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    U9513h
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    Packing:
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    Polyclonal Antibody for Human Transforming protein RhoA
    Polyclonal Antibody for Human Transforming protein RhoA
    Cat.:
    P9513Rb-h
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    Packing:
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    Polyclonal Antibody for Human Transforming protein RhoA
    Polyclonal Antibody for Human Transforming protein RhoA
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    P9513Rb-m
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Transforming protein RhoA
    Monoclonal Antibody for Human Transforming protein RhoA
    Monoclonal Antibody for Human Transforming protein RhoA
    Monoclonal Antibody for Human Transforming protein RhoA
    Monoclonal Antibody for Human Transforming protein RhoA
    Monoclonal Antibody for Human Transforming protein RhoA
    Protein for Human Transforming protein RhoA
    Protein for Human Transforming protein RhoA
    Protein for Human Transforming protein RhoA
    Protein for Human Transforming protein RhoA
    Protein for Human Transforming protein RhoA

    R&D Technical Data
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    Precision
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    Recovery
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    Linearity
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    References
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    2. 2.
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      tissue: Retina.
    3. 3.
      "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
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      tissue: Brain.
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      tissue: Adipose tissue.
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      tissue: Esophageal carcinoma.
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      "The DNA sequence, annotation and analysis of human chromosome 3."
      Muzny D.M. , Scherer S.E. , Kaul R. , Wang J. , Yu J. , Sudbrak R. , Buhay C.J. , Chen R. , Cree A. , Ding Y. , Dugan-Rocha S. , Gill R. , Gunaratne P. , Harris R.A. , Hawes A.C. , Hernandez J. , Hodgson A.V. , Hume J. , more...
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      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      tissue: Brain.
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      Moscow J.A. , He R. , Gudas J.M. , Cowan K.H.
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      tissue: Mammary cancer.
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      "A rho gene product in human blood platelets. I. Identification of the platelet substrate for botulinum C3 ADP-ribosyltransferase as rhoA protein."
      Nemoto Y. , Namba T. , Teru-uchi T. , Ushikubi F. , Morii N. , Narumiya S.
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      tissue: Platelet.
    11. 11.
      "Structure and function of the 5'-flanking sequence of the human cytosolic selenium-dependent glutathione peroxidase gene (hgpx1)."
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      Ishizaki T. , Maekawa M. , Fujisawa K. , Okawa K. , Iwamatsu A. , Fujita A. , Watanabe N. , Saito Y. , Kakizuka A. , Morii N. , Narumiya S.
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    13. 13.
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      Matsui T. , Amano M. , Yamamoto T. , Chihara K. , Nakafuku M. , Ito M. , Nakano T. , Okawa K. , Iwamatsu A. , Kaibuchi K.
      EMBO J.15:2208-2216(1996) [PubMed] [Europe PMC] [Abstract]
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      "Isolation of a NCK-associated kinase, PRK2, an SH3-binding protein and potential effector of Rho protein signaling."
      Quilliam L.A. , Lambert Q.T. , Mickelson-Young L.A. , Westwick J.K. , Sparks A.B. , Kay B.K. , Jenkins N.A. , Gilbert D.J. , Copeland N.G. , Der C.J.
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    15. 15.
      "The PRK2 kinase is a potential effector target of both Rho and Rac GTPases and regulates actin cytoskeletal organization."
      Vincent S. , Settleman J.
      Mol. Cell. Biol.17:2247-2256(1997) [PubMed] [Europe PMC] [Abstract]
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    16. 16.
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      Cachero T.G. , Morielli A.D. , Peralta E.G.
      Cell93:1077-1085(1998) [PubMed] [Europe PMC] [Abstract]
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    17. 17.
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      Ren Y. , Li R. , Zheng Y. , Busch H.
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      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH ARHGEF2
    18. 18.
      "Diacylglycerol kinase theta binds to and is negatively regulated by active RhoA."
      Houssa B. , de Widt J. , Kranenburg O. , Moolenaar W.H. , van Blitterswijk W.J.
      J. Biol. Chem.274:6820-6822(1999) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH DGKQ;MUTAGENESIS OF TYR-34
    19. 19.
      "RhoA interacts with the fusion glycoprotein of respiratory syncytial virus and facilitates virus-induced syncytium formation."
      Pastey M.K. , Crowe J.E. Jr. , Graham B.S.
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      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH HRSV PROTEIN F
    20. 20.
      "The PDZ protein TIP-1 interacts with the Rho effector rhotekin and is involved in Rho signaling to the serum response element."
      Reynaud C. , Fabre S. , Jalinot P.
      J. Biol. Chem.275:33962-33968(2000) [PubMed] [Europe PMC] [Abstract]
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    21. 21.
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    22. 22.
      "Ht31: the first protein kinase A anchoring protein to integrate protein kinase A and Rho signaling."
      Klussmann E. , Edemir B. , Pepperle B. , Tamma G. , Henn V. , Klauschenz E. , Hundsrucker C. , Maric K. , Rosenthal W.
      FEBS Lett.507:264-268(2001) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH AKAP13
    23. 23.
      "XPLN, a guanine nucleotide exchange factor for RhoA and RhoB, but not RhoC."
      Arthur W.T. , Ellerbroek S.M. , Der C.J. , Burridge K. , Wennerberg K.
      J. Biol. Chem.277:42964-42972(2002) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH ARHGEF3
    24. 24.
      "A Yersinia effector and a Pseudomonas avirulence protein define a family of cysteine proteases functioning in bacterial pathogenesis."
      Shao F. , Merritt P.M. , Bao Z. , Innes R.W. , Dixon J.E.
      Cell109:575-588(2002) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH YERSINIA PESTIS YOPT;CLEAVAGE
    25. 25.
      "Direct activation of phospholipase C-epsilon by Rho."
      Wing M.R. , Snyder J.T. , Sondek J. , Harden T.K.
      J. Biol. Chem.278:41253-41258(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH PLCE1
    26. 26.
      "Biochemical characterization of the Yersinia YopT protease: cleavage site and recognition elements in Rho GTPases."
      Shao F. , Vacratsis P.O. , Bao Z. , Bowers K.E. , Fierke C.A. , Dixon J.E.
      Proc. Natl. Acad. Sci. U.S.A.100:904-909(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH YERSINIA PSEUDOTUBERCULOSIS YOPT;CLEAVAGE;MUTAGENESIS OF LEU-193
    27. 27.
      "An ECT2-centralspindlin complex regulates the localization and function of RhoA."
      Yuce O. , Piekny A. , Glotzer M.
      J. Cell Biol.170:571-582(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;SUBCELLULAR LOCATION
    28. 28.
      "Dissecting the role of Rho-mediated signaling in contractile ring formation."
      Kamijo K. , Ohara N. , Abe M. , Uchimura T. , Hosoya H. , Lee J.S. , Miki T.
      Mol. Biol. Cell17:43-55(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;SUBCELLULAR LOCATION
    29. 29.
      "The armadillo protein p0071 regulates Rho signalling during cytokinesis."
      Wolf A. , Keil R. , Gotzl O. , Mun A. , Schwarze K. , Lederer M. , Huttelmaier S. , Hatzfeld M.
      Nat. Cell Biol.8:1432-1440(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH PKP4;SUBCELLULAR LOCATION
    30. 30.
      "The Rho-family GEF Asef2 activates Rac to modulate adhesion and actin dynamics and thereby regulate cell migration."
      Bristow J.M. , Sellers M.H. , Majumdar D. , Anderson B. , Hu L. , Webb D.J.
      J. Cell Sci.122:4535-4546(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
    31. 31.
      "Dual roles for RHOA/RHO-kinase in the regulated trafficking of a voltage-sensitive potassium channel."
      Stirling L. , Williams M.R. , Morielli A.D.
      Mol. Biol. Cell20:2991-3002(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
    32. 32.
      "The fic domain: regulation of cell signaling by adenylylation."
      Worby C.A. , Mattoo S. , Kruger R.P. , Corbeil L.B. , Koller A. , Mendez J.C. , Zekarias B. , Lazar C. , Dixon J.E.
      Mol. Cell34:93-103(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: AMPYLATION AT TYR-34;MUTAGENESIS OF TYR-34
    33. 33.
      "Cullin mediates degradation of RhoA through evolutionarily conserved BTB adaptors to control actin cytoskeleton structure and cell movement."
      Chen Y. , Yang Z. , Meng M. , Zhao Y. , Dong N. , Yan H. , Liu L. , Ding M. , Peng H.B. , Shao F.
      Mol. Cell35:841-855(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: UBIQUITINATION
    34. 34.
      "AMPylation of Rho GTPases by Vibrio VopS disrupts effector binding and downstream signaling."
      Yarbrough M.L. , Li Y. , Kinch L.N. , Grishin N.V. , Ball H.L. , Orth K.
      Science323:269-272(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: AMPYLATION AT THR-37
    35. 35.
      "Activated Rac1 GTPase translocates protein phosphatase 5 to the cell membrane and stimulates phosphatase activity in vitro."
      Chatterjee A. , Wang L. , Armstrong D.L. , Rossie S.
      J. Biol. Chem.285:3872-3882(2010) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: MUTAGENESIS OF GLY-14
    36. 36.
      "Regulation of Rho GTPase crosstalk, degradation and activity by RhoGDI1."
      Boulter E. , Garcia-Mata R. , Guilluy C. , Dubash A. , Rossi G. , Brennwald P.J. , Burridge K.
      Nat. Cell Biol.12:477-483(2010) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH ARHGDIA
    37. 37.
      "ErbB2 receptor controls microtubule capture by recruiting ACF7 to the plasma membrane of migrating cells."
      Zaoui K. , Benseddik K. , Daou P. , Salaun D. , Badache A.
      Proc. Natl. Acad. Sci. U.S.A.107:18517-18522(2010) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
    38. 38.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
    39. 39.
      "ARHGAP30 is a Wrch-1-interacting protein involved in actin dynamics and cell adhesion."
      Naji L. , Pacholsky D. , Aspenstrom P.
      Biochem. Biophys. Res. Commun.409:96-102(2011) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ENZYME REGULATION
    40. 40.
      "RACK1 promotes breast carcinoma migration/metastasis via activation of the RhoA/Rho kinase pathway."
      Cao X.X. , Xu J.D. , Xu J.W. , Liu X.L. , Cheng Y.Y. , Li Q.Q. , Xu Z.D. , Liu X.P.
      Breast Cancer Res. Treat.126:555-563(2011) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH GNB2L1
    41. 41.
      "The Rho target PRK2 regulates apical junction formation in human bronchial epithelial cells."
      Wallace S.W. , Magalhaes A. , Hall A.
      Mol. Cell. Biol.31:81-91(2011) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH PKN2
    42. 42.
      "Crystal structure of RhoA-GDP and its functional implications."
      Wei Y. , Zhang Y. , Derewenda U. , Liu X. , Minor W. , Nakamoto R.K. , Somlyo A.V. , Somlyo A.P. , Derewenda Z.S.
      Nat. Struct. Biol.4:699-703(1997) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS)
    43. 43.
      "Crystal structure of human RhoA in a dominantly active form complexed with a GTP analogue."
      Ihara K. , Muraguchi S. , Kato M. , Shimizu T. , Shirakawa M. , Kuroda S. , Kaibuchi K. , Hakoshima T.
      J. Biol. Chem.273:9656-9666(1998) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 4-181 OF MUTANT VAL-14
    44. 44.
      "Biochemical and crystallographic characterization of a Rho effector domain of the protein serine/threonine kinase N in a complex with RhoA."
      Maesaki R. , Shimizu T. , Ihara K. , Kuroda S. , Kaibuchi K. , Hakoshima T.
      J. Struct. Biol.126:166-170(1999) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 1-181 IN COMPLEX WITH PRKCL1
    45. 45.
      "An open conformation of switch I revealed by the crystal structure of a Mg2+-free form of RHOA complexed with GDP. Implications for the GDP/GTP exchange mechanism."
      Shimizu T. , Ihara K. , Maesaki R. , Kuroda S. , Kaibuchi K. , Hakoshima T.
      J. Biol. Chem.275:18311-18317(2000) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 3-180 IN COMPLEX WITH GDP
    46. 46.
      "MgF(3)(-) as a transition state analog of phosphoryl transfer."
      Graham D.L. , Lowe P.N. , Grime G.W. , Marsh M. , Rittinger K. , Smerdon S.J. , Gamblin S.J. , Eccleston J.F.
      Chem. Biol.9:375-381(2002) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS)
    47. 47.
      "Structural basis for the selective activation of Rho GTPases by Dbl exchange factors."
      Snyder J.T. , Worthylake D.K. , Rossman K.L. , Betts L. , Pruitt W.M. , Siderovski D.P. , Der C.J. , Sondek J.
      Nat. Struct. Biol.9:468-475(2002) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.81 ANGSTROMS) IN COMPLEX WITH MCF2
    48. 48.
      "Structure of a constitutively activated RhoA mutant (Q63L) at 1.55 A resolution."
      Longenecker K. , Read P. , Lin S.-K. , Somlyo A.P. , Nakamoto R.K. , Derewenda Z.S.
      Acta Crystallogr. D59:876-880(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF MUTANT LEU-63 IN COMPLEX WITH A GTP ANALOG AND MG(2+)
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