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Index > Protein center > S100A4(Gene name) > Human
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  • S100A4 (Gene name),
  • Protein S100-A4 (Protein name ),  S10A4_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Predicted Eptitope
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  • Gene name:
    S100A4(CAPL;MTS1);
    Protein name:
    Protein S100-A4;
    Alternative:
    Metastasin;Calvasculin;Protein Mts1;Placental calcium-binding protein;S100 calcium-binding protein A4;
    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    Homodimer. Interacts with PPFIBP1 in a calcium-dependent mode.
    Function:
    N/A
    Subcellular Location:
    N/A
    Protein Attributes:
    Sequence length:
    101
    Sequence:
    50:
    MACPLEKALD | VMVSTFHKYS | GKEGDKFKLN | KSELKELLTR | ELPSFLGKRT | 
    100:
    DEAAFQKLMS | NLDSNRDNEV | DFQEYCVFLS | CIAMMCNEFF | EGFPDKQPRK | 
    101:
    K
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
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    Related Databases
    MIM:
    UniGene:
    KEGG:
    SMR:
    Pfam:
    String:
    Uniprot:
     
    FOR
    ELISA Kit for Human Protein S100-A4
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    E1399m
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    96T
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    ELISA Kit for Human Protein S100-A4
    Cat.:
    E1399h
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    96T
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    ELISA Kit for Human Protein S100-A4
    Cat.:
    E1399r
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    96T
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    ELISA Kit for Human Protein S100-A4
    Cat.:
    E1399d
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    MSDS:
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    Packing:
    96T
    ELISA Kit for Human Protein S100-A4
    Cat.:
    E1399b
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    Packing:
    96T
    CLIA Kit for Human Protein S100-A4
    Cat.:
    U1399d
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    MSDS:
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    Packing:
    96T
    CLIA Kit for Human Protein S100-A4
    Cat.:
    U1399h
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    96T
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    CLIA Kit for Human Protein S100-A4
    Cat.:
    U1399r
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    Packing:
    96T
    CLIA Kit for Human Protein S100-A4
    Cat.:
    U1399m
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    Packing:
    96T
    CLIA Kit for Human Protein S100-A4
    Cat.:
    U1399b
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    MSDS:
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    Packing:
    96T
    Polyclonal Antibody for Human Protein S100-A4
    Cat.:
    P1399Rb-h
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Protein S100-A4
    Polyclonal Antibody for Human Protein S100-A4
    Polyclonal Antibody for Human Protein S100-A4
    Cat.:
    P1399Rb-m
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    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Protein S100-A4
    Cat.:
    P1399Rb-r
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    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Human Protein S100-A4
    Monoclonal Antibody for Human Protein S100-A4
    Monoclonal Antibody for Human Protein S100-A4
    Monoclonal Antibody for Human Protein S100-A4
    Monoclonal Antibody for Human Protein S100-A4
    Protein for Human Protein S100-A4
    Protein for Human Protein S100-A4
    Protein for Human Protein S100-A4
    Protein for Human Protein S100-A4
    Protein for Human Protein S100-A4

    R&D Technical Data
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    s

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    s
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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤7.6%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤10.9%

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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

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    Recovery
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    Recovery was determined by spiking various levels of S10A4 into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    98

    90-104

    Plasma

    99

    93-108

     

     

     

     

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    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of S10A4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    90-106%

    93-104%

    94-102%

    92-106%

    EDTA plasma(n=5)

    91-108%

    90-107%

    96-102%

    94-105%

    heparin plasma(n=5)

    95-105%

     

    92-105%

    91-106%

    93-103%

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    EDTA plasma(n=5)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    References
    1. 1.
      "S100 alpha, CAPL, and CACY: molecular cloning and expression analysis of three calcium-binding proteins from human heart."
      Engelkamp D. , Schaefer B.W. , Erne P. , Heizmann C.W.
      Biochemistry31:10258-10264(1992) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
      tissue: Heart.
    2. 2.
      "Six S100 genes are clustered on human chromosome 1q21: identification of two genes coding for the two previously unreported calcium-binding proteins S100D and S100E."
      Engelkamp D. , Schaefer B.W. , Mattei M.-G. , Erne P. , Heizmann C.W.
      Proc. Natl. Acad. Sci. U.S.A.90:6547-6551(1993) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    3. 3.
      "Transcriptional analysis of the mts1 gene with specific reference to 5' flanking sequences."
      Tulchinsky E.M. , Ford H.L. , Kramerov D. , Reshetnyak E. , Grigorian M. , Zain S. , Lukanidin E.
      Proc. Natl. Acad. Sci. U.S.A.89:9146-9150(1992) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
      tissue: Spleen.
    4. 4.
      "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L. , Schick M. , Neubert P. , Schatten R. , Henze S. , Korn B.
      Submitted (2004-05) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
    5. 5.
      "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
      Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      tissue: Synovium.
    6. 6.
      "The DNA sequence and biological annotation of human chromosome 1."
      Gregory S.G. , Barlow K.F. , McLay K.E. , Kaul R. , Swarbreck D. , Dunham A. , Scott C.E. , Howe K.L. , Woodfine K. , Spencer C.C.A. , Jones M.C. , Gillson C. , Searle S. , Zhou Y. , Kokocinski F. , McDonald L. , Evans R. , Phillips K. , more...
      Nature441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    7. 7.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    8. 8.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      tissue: Cervix.
      tissue: Prostate.
    9. 9.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 8-18 AND 36-57
      tissue: Platelet.
    10. 10.
      "Liprin beta 1, a member of the family of LAR transmembrane tyrosine phosphatase-interacting proteins, is a new target for the metastasis-associated protein S100A4 (Mts1)."
      Kriajevska M. , Fischer-Larsen M. , Moertz E. , Vorm O. , Tulchinsky E. , Grigorian M. , Ambartsumian N. , Lukanidin E.
      J. Biol. Chem.277:5229-5235(2002) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH PPFIBP1
    11. 11.
      "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
      Choudhary C. , Kumar C. , Gnad F. , Nielsen M.L. , Rehman M. , Walther T.C. , Olsen J.V. , Mann M.
      Science325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2; LYS-7 AND LYS-35;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS];CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS]
    12. 12.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
    13. 13.
      "Solution structure of human Mts1 (S100A4) as determined by NMR spectroscopy."
      Vallely K.M. , Rustandi R.R. , Ellis K.C. , Varlamova O. , Bresnick A.R. , Weber D.J.
      Biochemistry41:12670-12680(2002) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR
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