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Index > Protein center > S100A6(Gene name) > Human
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  • S100A6 (Gene name),
  • Protein S100-A6 (Protein name ),  S10A6_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • 3D
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Gene name:
    S100A6(CACY);
    Protein name:
    Protein S100-A6;
    Alternative:
    Growth factor-inducible protein 2A9;Calcyclin;Prolactin receptor-associated protein(PRA);MLN 4;S100 calcium-binding protein A6;
    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    Homodimer; head to tail assembly of 2 subunits. Interacts with CACYBP (By similarity). Interacts with ANXA2 and ANXA11 (via N-terminus). Interacts with SUGT1. Interacts with TP53; has higher affinity for TP53 that is phosphorylated on its N-terminal domain, and lower affinity for TP53 that is phosphorylated on its C-terminal domain. Interacts with tropomyosin. Interacts with FKBP4.
    Function:
    May function as calcium sensor and contribute to cellular calcium signaling (Potential). May function by interacting with other proteins and indirectly play a role in the reorganization of the actin cytoskeleton and in cell motility. Binds 2 calcium ions. Calcium binding is cooperative.
    Subcellular Location:
    Nucleus envelope Cytoplasm Cell membrane Peripheral membrane protein Cytoplasmic side
    Protein Attributes:
    Sequence length:
    90
    Sequence:
    50:
    MACPLDQAIG | LLVAIFHKYS | GREGDKHTLS | KKELKELIQK | ELTIGSKLQD | 
    90:
    AEIARLMEDL | DRNKDQEVNF | QEYVTFLGAL | ALIYNEALKG | 
    3D Structure:
    N/A
    Predicted Eptitope:
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    EIAab Sequence  Vaild Sequence:
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    Related Databases
    String:
    UniGene:
    KEGG:
    MIM:
    SMR:
    Pfam:
    Uniprot:
     
    FOR
    ELISA Kit for Human Protein S100-A6
    Cat.:
    E1769h
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    96T
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    ELISA Kit for Human Protein S100-A6
    Cat.:
    E1769p
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    Packing:
    96T
    ELISA Kit for Human Protein S100-A6
    Cat.:
    E1769r
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    MSDS:
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    Packing:
    96T
    ELISA Kit for Human Protein S100-A6
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    ELISA Kit for Human Protein S100-A6
    Cat.:
    E1769m
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    Packing:
    96T
    ELISA Kit for Human Protein S100-A6
    Cat.:
    E1769c
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    Packing:
    96T
    CLIA Kit for Human Protein S100-A6
    Cat.:
    U1769p
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    MSDS:
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    Packing:
    96T
    CLIA Kit for Human Protein S100-A6
    Cat.:
    U1769r
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    MSDS:
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    Packing:
    96T
    CLIA Kit for Human Protein S100-A6
    Cat.:
    U1769m
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    96T
    CLIA Kit for Human Protein S100-A6
    Cat.:
    U1769c
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    CLIA Kit for Human Protein S100-A6
    Cat.:
    U1769h
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    CLIA Kit for Human Protein S100-A6
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    Polyclonal Antibody for Human Protein S100-A6
    Cat.:
    P1769Rb-h
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Protein S100-A6
    Polyclonal Antibody for Human Protein S100-A6
    Polyclonal Antibody for Human Protein S100-A6
    Polyclonal Antibody for Human Protein S100-A6
    Cat.:
    P1769Rb-r
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Protein S100-A6
    Cat.:
    P1769Rb-m
    Price:
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    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Human Protein S100-A6
    Monoclonal Antibody for Human Protein S100-A6
    Monoclonal Antibody for Human Protein S100-A6
    Monoclonal Antibody for Human Protein S100-A6
    Monoclonal Antibody for Human Protein S100-A6
    Monoclonal Antibody for Human Protein S100-A6
    Protein for Human Protein S100-A6
    Protein for Human Protein S100-A6
    Protein for Human Protein S100-A6
    Protein for Human Protein S100-A6
    Protein for Human Protein S100-A6
    Protein for Human Protein S100-A6

    R&D Technical Data
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    s
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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

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    Recovery
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    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    EDTA plasma(n=5)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

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    References
    1. 1.
      "Molecular cloning of the cDNA for a growth factor-inducible gene with strong homology to S-100, a calcium-binding protein."
      Calabretta B. , Battini R. , Kaczmarek L. , de Riel J.K. , Baserga R.
      J. Biol. Chem.261:12628-12632(1986) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
      tissue: Fibroblast.
    2. 2.
      "Structural and functional analysis of a growth-regulated gene, the human calcyclin."
      Ferrari S. , Calabretta B. , Deriel J.K. , Battini R. , Ghezzo F. , Lauret E. , Griffin C. , Emanuel B.S. , Gurrieri F. , Baserga R.
      J. Biol. Chem.262:8325-8332(1987) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    3. 3.
      "Cloning and characterization of a cDNA encoding a highly conserved, putative calcium binding protein, identified by an anti-prolactin receptor antiserum."
      Murphy L.C. , Murphy L.J. , Tsuyuki D. , Duckworth M.L. , Shiu R.P.C.
      J. Biol. Chem.263:2397-2401(1988) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
    4. 4.
      "Cloning of human calcyclin and calcyclin binding protein (CacyBP)."
      Wu J. , Liu W. , Zhou Y. , Zhao Z. , Peng X. , Yuan J. , Qiang B.
      Submitted (2001-05) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    5. 5.
      "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N. , Chen X. , Rolfs A. , Halleck A. , Hines L. , Eisenstein S. , Koundinya M. , Raphael J. , Moreira D. , Kelley T. , LaBaer J. , Lin Y. , Phelan M. , Farmer A.
      Submitted (2003-05) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
    6. 6.
      "The DNA sequence and biological annotation of human chromosome 1."
      Gregory S.G. , Barlow K.F. , McLay K.E. , Kaul R. , Swarbreck D. , Dunham A. , Scott C.E. , Howe K.L. , Woodfine K. , Spencer C.C.A. , Jones M.C. , Gillson C. , Searle S. , Zhou Y. , Kokocinski F. , McDonald L. , Evans R. , Phillips K. , more...
      Nature441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    7. 7.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    8. 8.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      tissue: Brain.
      tissue: Placenta.
    9. 9.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 27-31 AND 48-89
      tissue: Platelet.
    10. 10.
      "Identification of a cell cycle-dependent gene product as a sialic acid-binding protein."
      Gabius H.J. , Bardosi A. , Gabius S. , Hellmann K.P. , Karas M. , Kratzin H.
      Biochem. Biophys. Res. Commun.163:506-512(1989) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 57-74
    11. 11.
      "Calcium-regulated interaction of Sgt1 with S100A6 (calcyclin) and other S100 proteins."
      Nowotny M. , Spiechowicz M. , Jastrzebska B. , Filipek A. , Kitagawa K. , Kuznicki J.
      J. Biol. Chem.278:26923-26928(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH SUGT1
    12. 12.
      "Calcium- and cell cycle-dependent association of annexin 11 with the nuclear envelope."
      Tomas A. , Moss S.E.
      J. Biol. Chem.278:20210-20216(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION
    13. 13.
      "S100A6 binds to annexin 2 in pancreatic cancer cells and promotes pancreatic cancer cell motility."
      Nedjadi T. , Kitteringham N. , Campbell F. , Jenkins R.E. , Park B.K. , Navarro P. , Ashcroft F. , Tepikin A. , Neoptolemos J.P. , Costello E.
      Br. J. Cancer101:1145-1154(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH ANXA2; ANXA11 AND TROPOMYOSIN;SUBCELLULAR LOCATION
    14. 14.
      "Posttranslational modifications affect the interaction of S100 proteins with tumor suppressor p53."
      van Dieck J. , Teufel D.P. , Jaulent A.M. , Fernandez-Fernandez M.R. , Rutherford T.J. , Wyslouch-Cieszynska A. , Fersht A.R.
      J. Mol. Biol.394:922-930(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH TP53
    15. 15.
      "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
      Choudhary C. , Kumar C. , Gnad F. , Nielsen M.L. , Rehman M. , Walther T.C. , Olsen J.V. , Mann M.
      Science325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-40;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
    16. 16.
      "S100 proteins regulate the interaction of Hsp90 with cyclophilin 40 and FKBP52 through their tetratricopeptide repeats."
      Shimamoto S. , Kubota Y. , Tokumitsu H. , Kobayashi R.
      FEBS Lett.584:1119-1125(2010) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH FKBP4
    17. 17.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
    18. 18.
      "S100 proteins modulate protein phosphatase 5 function: a link between CA2+ signal transduction and protein dephosphorylation."
      Yamaguchi F. , Umeda Y. , Shimamoto S. , Tsuchiya M. , Tokumitsu H. , Tokuda M. , Kobayashi R.
      J. Biol. Chem.287:13787-13798(2012) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH PPP5C;SUBCELLULAR LOCATION
    19. 19.
      "Crystal structures of S100A6 in the Ca(2+)-free and Ca(2+)-bound states: the calcium sensor mechanism of S100 proteins revealed at atomic resolution."
      Otterbein L.R. , Kordowska J. , Witte-Hoffmann C. , Wang C.-L.A. , Dominguez R.
      Structure10:557-567(2002) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.15 ANGSTROMS);SUBUNIT;CALCIUM-BINDING
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