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12% SDS-PAGE analysis
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Precision
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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.3%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.2%
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Recovery
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Recovery was determined by spiking various levels of SAA1 into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
101
95-103
Plasma
99
93-106
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Linearity
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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SAA1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
94-106%
96-107%
95-109%
97-105%
EDTA plasma(n=5)
91-108%
95-103%
92-107%
93-106%
heparin plasma(n=5)
93-104%
94-106%
90-109%
96-104%
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[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ALLELE SAA1.5)
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ALLELE SAA1.5)
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ALLELE SAA1.5)
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ALLELE SAA1.1)
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 73-122;VARIANTS VAL-75 AND ASN-78
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: PROTEIN SEQUENCE OF 19-122;SUBCELLULAR LOCATION;SUBUNIT;TISSUE SPECIFICITY
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: PROTEIN SEQUENCE OF 19-94 (FAMILIAL MEDITERRANEAN FEVER PATIENT)
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: PROTEIN SEQUENCE OF 19-94;MASS SPECTROMETRY;DISEASE;VARIANTS ALA-70; VAL-75 AND ASN-78;ALLELE SAA1.3
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: PROTEIN SEQUENCE OF 20-33; 44-57; 64-80 AND 86-122;TISSUE SPECIFICITY;MASS SPECTROMETRY
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: PARTIAL PROTEIN SEQUENCE (VARIOUS FORMS);METHYLATION AT ASN-101
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: X-RAY CRYSTALLOGRAPHY (2.19 ANGSTROMS) OF 19-122;SUBUNIT;MUTAGENESIS OF ARG-19; ARG-33; ARG-37; ARG-65; ARG-80 AND HIS-89
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