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Index > Protein center > AANAT(Gene name) > Human
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  • AANAT (Gene name),
  • Serotonin N-acetyltransferase (Protein name ),  SNAT_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Gene name:
    AANAT(SNAT);
    Protein name:
    Serotonin N-acetyltransferase(Serotonin acetylase);
    Alternative:
    Aralkylamine N-acetyltransferase(AA-NAT);
    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    Monomer (By similarity). Interacts with YWHAZ; the interaction requires phosphorylation on Thr-31, and modulates the enzymatic activity of AANAT through preventing dephosphorylation and/or proteolysis and stabilizing substrate binding. Subsequently, a second molecule of AANAT can bind, via the phosphorylated Ser-205 site, the other YWHAZ monomer with similar effect.
    Function:
    Catalyzes the N-acetylation of serotonin into N-acetylserotonin. Controls the night/day rhythm of melatonin production in the pineal gland. Has a relative affinity for hydroxylated versus non-hydroxylated arylalkylamines.
    Subcellular Location:
    Cytoplasm
    Protein Attributes:
    Sequence length:
    207
    Sequence:
    50:
    MSTQSTHPLK | PEAPRLPPGI | PESPSCQRRH | TLPASEFRCL | TPEDAVSAFE | 
    100:
    IEREAFISVL | GVCPLYLDEI | RHFLTLCPEL | SLGWFEEGCL | VAFIIGSLWD | 
    150:
    KERLMQESLT | LHRSGGHIAH | LHVLAVHRAF | RQQGRGPILL | WRYLHHLGSQ | 
    200:
    PAVRRAALMC | EDALVPFYER | FSFHAVGPCA | ITVGSLTFME | LHCSLRGHPF | 
    207:
    LRRNSGC
    3D Structure:
    N/A
    Predicted Eptitope:
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    EIAab Sequence  Vaild Sequence:
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    Related Databases
    SMR:
    String:
    UniGene:
    MIM:
    Pfam:
    KEGG:
    Uniprot:
     
    FOR
    ELISA Kit for Human Serotonin acetylase
    Cat.:
    E1937r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human Serotonin acetylase
    Cat.:
    E1937h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human Serotonin acetylase
    Cat.:
    E1937b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    ELISA Kit for Human Serotonin acetylase
    Cat.:
    E1937c
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    ELISA Kit for Human Serotonin acetylase
    Cat.:
    E1937m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Serotonin acetylase
    Cat.:
    U1937m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Serotonin acetylase
    Cat.:
    U1937b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Serotonin acetylase
    Cat.:
    U1937c
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Serotonin acetylase
    Cat.:
    U1937h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human Serotonin acetylase
    Cat.:
    U1937r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    Polyclonal Antibody for Human Serotonin acetylase
    Polyclonal Antibody for Human Serotonin acetylase
    Cat.:
    P1937Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Serotonin acetylase
    Cat.:
    P1937Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Serotonin acetylase
    Polyclonal Antibody for Human Serotonin acetylase
    Cat.:
    P1937Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Human Serotonin acetylase
    Monoclonal Antibody for Human Serotonin acetylase
    Monoclonal Antibody for Human Serotonin acetylase
    Monoclonal Antibody for Human Serotonin acetylase
    Monoclonal Antibody for Human Serotonin acetylase
    Protein for Human Serotonin acetylase
    Protein for Human Serotonin acetylase
    Protein for Human Serotonin acetylase
    Protein for Human Serotonin acetylase
    Protein for Human Serotonin acetylase

    R&D Technical Data

    s

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    s
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    Precision

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤6.8%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤10.7%

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    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

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    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    Recovery

    Recovery was determined by spiking various levels of SNAT into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    94-100

    Plasma

    98

    96-102

     

     

     

     

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    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

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    Linearity

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SNAT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-108%

    95-102%

    92-107%

    EDTA plasma(n=5)

    94-103%

    96-101%

    93-105%

    95-102%

    heparin plasma(n=5)

    93-104%

     

    94-104%

    95-103%

    91-105%

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    EDTA plasma(n=5)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    References
    1. 1.
      "The human serotonin N-acetyltransferase (EC 2.3.1.87) gene (AANAT): structure, chromosomal localization, and tissue expression."
      Coon S.L. , Mazuruk K. , Bernard M. , Roseboom P. , Klein D.C. , Rodriguez I.R.
      Genomics34:76-84(1996) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1);INDUCTION;TISSUE SPECIFICITY
    2. 3.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      tissue: Brain.
      tissue: Testis.
    3. 4.
      "cAMP regulation of arylalkylamine N-acetyltransferase (AANAT, EC 2.3.1.87): a new cell line (1E7) provides evidence of intracellular AANAT activation."
      Coon S.L. , Weller J.L. , Korf H.-W. , Namboodiri M.A. , Rollag M. , Klein D.C.
      J. Biol. Chem.276:24097-24107(2001) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION;INDUCTION;BIOPHYSICOCHEMICAL PROPERTIES
    4. 5.
      "Role of a pineal cAMP-operated arylalkylamine N-acetyltransferase/14-3-3-binding switch in melatonin synthesis."
      Ganguly S. , Gastel J.A. , Weller J.L. , Schwartz C. , Jaffe H. , Namboodiri M.A. , Coon S.L. , Hickman A.B. , Rollag M. , Obsil T. , Beauverger P. , Ferry G. , Boutin J.A. , Klein D.C.
      Proc. Natl. Acad. Sci. U.S.A.98:8083-8088(2001) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH 14-3-3 PROTEINS;MUTAGENESIS OF THR-31
    5. 6.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: TISSUE SPECIFICITY
    6. 7.
      "Significant association of the arylalkylamine N-acetyltransferase (AA-NAT) gene with delayed sleep phase syndrome."
      Hohjoh H. , Takasu M. , Shishikura K. , Takahashi Y. , Honda Y. , Tokunaga K.
      Neurogenetics4:151-153(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT DSPS THR-129
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