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Index > Protein center > SNCA(Gene name) > Human
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  • SNCA (Gene name),
  • Alpha-synuclein (Protein name ),  SYUA_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Gene name:
    SNCA(NACP;PARK1);
    Protein name:
    Alpha-synuclein;
    Alternative:
    Non-A4 component of amyloid precursor(NACP);Non-A beta component of AD amyloid;
    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    Soluble monomer which can form filamentous aggregates. Interacts with UCHL1 (By similarity). Interacts with phospholipase D and histones.
    Function:
    May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.
    Subcellular Location:
    Cytoplasm Membrane Nucleus Cell junction synapse Membrane-bound in dopaminergic neurons.
    Protein Attributes:
    Sequence length:
    140
    Sequence:
    50:
    MDVFMKGLSK | AKEGVVAAAE | KTKQGVAEAA | GKTKEGVLYV | GSKTKEGVVH | 
    100:
    GVATVAEKTK | EQVTNVGGAV | VTGVTAVAQK | TVEGAGSIAA | ATGFVKKDQL | 
    140:
    GKNEEGAPQE | GILEDMPVDP | DNEAYEMPSE | EGYQDYEPEA | 
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    MIM:
    String:
    UniGene:
    SMR:
    KEGG:
    Pfam:
    Uniprot:
     
    FOR
    ELISA Kit for Human Alpha-synuclein
    Cat.:
    E1222m
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    96T
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    ELISA Kit for Human Alpha-synuclein
    Cat.:
    E1222r
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    96T
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    ELISA Kit for Human Alpha-synuclein
    Cat.:
    E1222h
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    ELISA Kit for Human Alpha-synuclein
    Cat.:
    E1222b
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    Packing:
    96T
    ELISA Kit for Human Alpha-synuclein
    Cat.:
    E1222p
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    MSDS:
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    Packing:
    96T
    CLIA Kit for Human Alpha-synuclein
    Cat.:
    U1222b
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    MSDS:
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    Packing:
    96T
    CLIA Kit for Human Alpha-synuclein
    Cat.:
    U1222h
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    MSDS:
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    Packing:
    96T
    Range:
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    CLIA Kit for Human Alpha-synuclein
    Cat.:
    U1222r
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    MSDS:
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    Packing:
    96T
    CLIA Kit for Human Alpha-synuclein
    Cat.:
    U1222p
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    MSDS:
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    Packing:
    96T
    CLIA Kit for Human Alpha-synuclein
    Cat.:
    U1222m
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    MSDS:
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    Packing:
    96T
    Polyclonal Antibody for Human Alpha-synuclein
    Cat.:
    P1222Rb-h
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Alpha-synuclein
    Cat.:
    P1222Rb-r
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Alpha-synuclein
    Cat.:
    P1222Rb-m
    Price:
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Alpha-synuclein
    Polyclonal Antibody for Human Alpha-synuclein
    Monoclonal Antibody for Human Alpha-synuclein
    Monoclonal Antibody for Human Alpha-synuclein
    Monoclonal Antibody for Human Alpha-synuclein
    Monoclonal Antibody for Human Alpha-synuclein
    Monoclonal Antibody for Human Alpha-synuclein
    Protein for Human Alpha-synuclein
    Protein for Human Alpha-synuclein
    Protein for Human Alpha-synuclein
    Protein for Human Alpha-synuclein
    Protein for Human Alpha-synuclein

    R&D Technical Data
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         12% SDS-PAGE Analysis

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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤ 3.7%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤ 6.1%

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    Recovery
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    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    94

    91-98

    Plasma

    93

    93-99

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-96%

    90-99%

    91-101%

    92-104%

    EDTA plasma(n=5)

    89-95%

    93-101%

    89-94%

    90-102%

    heparin plasma(n=5)

    93-102%

     

    94-103%

    96-102%

    91-101%

     

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    References
    1. 1.
      "Molecular cloning of cDNA encoding an unrecognized component of amyloid in Alzheimer disease."
      Ueda K. , Fukushima H. , Masliah E. , Xia Y. , Iwai A. , Yoshimoto M. , Otero D.A. , Kondo J. , Ihara Y. , Saitoh T.
      Proc. Natl. Acad. Sci. U.S.A.90:11282-11286(1993) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);PROTEIN SEQUENCE OF 61-95
      tissue: Brain.
    2. 2.
      "The NACP/synuclein gene: chromosomal assignment and screening for alterations in Alzheimer disease."
      Campion D. , Martin C. , Heilig R. , Charbonnier F. , Moreau V. , Flaman J.-M. , Petit J.-L. , Hannequin D. , Brice A. , Frebourg T.
      Genomics26:254-257(1995) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2-4 AND 2-5)
    3. 3.
      "Tissue-dependent alternative splicing of mRNA for NACP, the precursor of non-A beta component of Alzheimer's disease amyloid."
      Ueda K. , Saitoh T. , Mori H.
      Biochem. Biophys. Res. Commun.205:1366-1372(1994) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2-4)
      tissue: Brain.
    4. 4.
      Xia Y. , Silva R.D. , Chen X.H. , Saitoh T.
      Submitted (1996-01) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    5. 5.
      "Human and mouse alpha-synuclein genes: comparative genomic sequence analysis and identification of a novel gene regulatory element."
      Touchman J.W. , Dehejia A. , Chiba-Falek O. , Cabin D.E. , Schwartz J.R. , Orrison B.M. , Polymeropoulos M.H. , Nussbaum R.L.
      Genome Res.11:78-86(2001) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1 AND 2-4)
    6. 6.
      Hu X. , Xu Y. , Peng X. , Yuan J. , Qiang B.
      Submitted (2001-07) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
    7. 7.
      "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
      Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
      tissue: Thalamus.
    8. 8.
      "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L. , Schick M. , Neubert P. , Schatten R. , Henze S. , Korn B.
      Submitted (2004-06) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
    9. 9.
      NIEHS SNPs program
      Submitted (2005-06) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    10. 10.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    11. 11.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
      tissue: Uterus.
    12. 12.
      Lubec G. , Chen W.-Q. , Sun Y.
      Submitted (2008-12) to the UniProtKB
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 59-96;IDENTIFICATION BY MASS SPECTROMETRY
      tissue: Fetal brain cortex.
    13. 13.
      "Constitutive phosphorylation of the Parkinson's disease associated alpha-synuclein."
      Okochi M. , Walter J. , Koyama A. , Nakajo S. , Baba M. , Iwatsubo T. , Meijer L. , Kahle P.J. , Haass C.
      J. Biol. Chem.275:390-397(2000) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT SER-87 AND SER-129 BY CK1 AND CK2
    14. 14.
      "Synucleins are a novel class of substrates for G protein-coupled receptor kinases."
      Pronin A.N. , Morris A.J. , Surguchov A. , Benovic J.L.
      J. Biol. Chem.275:26515-26522(2000) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION BY G-PROTEIN COUPLED RECEPTOR KINASE
    15. 15.
      "Activated Fyn phosphorylates alpha-synuclein at tyrosine residue 125."
      Nakamura T. , Yamashita H. , Takahashi T. , Nakamura S.
      Biochem. Biophys. Res. Commun.280:1085-1092(2001) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT TYR-125 BY FYN
    16. 16.
      "Alpha-synuclein interacts with phospholipase D isozymes and inhibits pervanadate-induced phospholipase D activation in human embryonic kidney-293 cells."
      Ahn B.H. , Rhim H. , Kim S.Y. , Sung Y.M. , Lee M.Y. , Choi J.Y. , Wolozin B. , Chang J.S. , Lee Y.H. , Kwon T.K. , Chung K.C. , Yoon S.H. , Hahn S.J. , Kim M.S. , Jo Y.H. , Min do S.
      J. Biol. Chem.277:12334-12342(2002) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH PHOSPHOLIPASE D
    17. 17.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT SER-129
    18. 18.
      "Nuclear localization of alpha-synuclein and its interaction with histones."
      Goers J. , Manning-Bog A.B. , McCormack A.L. , Millett I.S. , Doniach S. , Di Monte D.A. , Uversky V.N. , Fink A.L.
      Biochemistry42:8465-8471(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH HISTONES;SUBCELLULAR LOCATION
    19. 19.
      "Role of alpha-synuclein carboxy-terminus on fibril formation in vitro."
      Murray I.V. , Giasson B.I. , Quinn S.M. , Koppaka V. , Axelsen P.H. , Ischiropoulos H. , Trojanowski J.Q. , Lee V.M.
      Biochemistry42:8530-8540(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ROLE OF THE C-TERMINUS IN FIBRILLOGENESIS
    20. 20.
      "Recent advances on alpha-synuclein cell biology: functions and dysfunctions."
      Alves da Costa C.
      Curr. Mol. Med.3:17-24(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW
    21. 21.
      "Identification and characterization of a novel Pyk2/related adhesion focal tyrosine kinase-associated protein that inhibits alpha-synuclein phosphorylation."
      Takahashi T. , Yamashita H. , Nagano Y. , Nakamura T. , Ohmori H. , Avraham H. , Avraham S. , Yasuda M. , Matsumoto M.
      J. Biol. Chem.278:42225-42233(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: MUTAGENESIS OF TYR-39; TYR-125; TYR-133 AND TYR-136;CHARACTERIZATION OF VARIANT THR-53;PHOSPHORYLATION AT TYR-125
    22. 22.
      "Lipid rafts mediate the synaptic localization of alpha-synuclein."
      Fortin D.L. , Troyer M.D. , Nakamura K. , Kubo S. , Anthony M.D. , Edwards R.H.
      J. Neurosci.24:6715-6723(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION
    23. 23.
      "Characterization of hydrophobic residue requirements for alpha-synuclein fibrillization."
      Waxman E.A. , Mazzulli J.R. , Giasson B.I.
      Biochemistry48:9427-9436(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FIBRILS FORMATION;DOMAIN NAC;MUTAGENESIS OF 67-GLY--VAL-71; 71-VAL--VAL-82; 76-ALA-VAL-77; VAL-77; ALA-78 AND 85-ALA--PHE-94
    24. 24.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
    25. 25.
      "Coordination features and affinity of the Cu(2)+ site in the alpha-synuclein protein of Parkinson's disease."
      Dudzik C.G. , Walter E.D. , Millhauser G.L.
      Biochemistry50:1771-1777(2011) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: COPPER-BINDING;MUTAGENESIS OF ASP-2 AND HIS-50
    26. 26.
      "N-Terminal acetylation is critical for forming alpha-helical oligomer of alpha-synuclein."
      Trexler A.J. , Rhoades E.
      Protein Sci.21:601-605(2012) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION AT MET-1
    27. 27.
      "Structure and dynamics of micelle-bound human alpha-synuclein."
      Ulmer T.S. , Bax A. , Cole N.B. , Nussbaum R.L.
      J. Biol. Chem.280:9595-9603(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR IN COMPLEX WITH DETERGENT MICELLES
    28. 28.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT PARK1 THR-53
    29. 29.
      "Ala30Pro mutation in the gene encoding alpha-synuclein in Parkinson's disease."
      Krueger R. , Kuhn W. , Mueller T. , Woitalla D. , Graeber M. , Koesel S. , Przuntek H. , Epplen J.T. , Schoels L. , Riess O.
      Nat. Genet.18:106-108(1998) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT PARK1 PRO-30
    30. 30.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT PARK1/DLB LYS-46
    31. 31.
      "Mutation E46K increases phospholipid binding and assembly into filaments of human alpha-synuclein."
      Choi W. , Zibaee S. , Jakes R. , Serpell L.C. , Davletov B. , Crowther R.A. , Goedert M.
      FEBS Lett.576:363-368(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: CHARACTERIZATION OF VARIANT LYS-46
    32. 32.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT PARK1 GLN-50
    33. 33.
      "A novel alpha-synuclein missense mutation in Parkinson disease."
      Proukakis C. , Dudzik C.G. , Brier T. , MacKay D.S. , Cooper J.M. , Millhauser G.L. , Houlden H. , Schapira A.H.
      Neurology80:1062-1064(2013) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT PARK1 GLN-50;CHARACTERIZATION OF VARIANT PARK1 GLN-50
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