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Index > Protein center > Timp1(Gene name) > Rat
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  • Timp1 (Gene name),
  • Metalloproteinase inhibitor 1 (Protein name ),  TIMP1_RAT from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Gene name:
    Timp1(Timp-1);
    Protein name:
    Metalloproteinase inhibitor 1;
    Alternative:
    Tissue inhibitor of metalloproteinases 1(TIMP-1);
    Organism:
    Rat (Rattus norvegicus). 
    General Annotation
    Sub Unit:
    N/A
    Function:
    Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also stimulates steroidogenesis by Leydig and ovarian granuloma cells; procathepsin L is required for maximal activity.
    Subcellular Location:
    Secreted
    Protein Attributes:
    Sequence length:
    217
    Sequence:
    50:
    MAPFASLASG | ILLLLSLIAS | SKACSCAPTH | PQTAFCNSDL | VIRAKFMGSP | 
    100:
    EIIETTLYQR | YEIKMTKMLK | GFDAVGNATG | FRFAYTPAME | SLCGYVHKSQ | 
    150:
    NRSEEFLIAG | RLRNGNLHIT | ACSFLVPWHN | LSPAQQKAFV | KTYSAGCGVC | 
    200:
    TVFPCSAIPC | KLESDSHCLW | TDQILMGSEK | GYQSDHFACL | PRNPDLCTWQ | 
    217:
    YLGVSMTRSL | PLAKAEA
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
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    Related Databases
    Pfam:
    String:
    SMR:
    UniGene:
    Uniprot:
     
    FOR
    ELISA Kit for Rat Metalloproteinase inhibitor 1
    Cat.:
    E0552m
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    96T
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    ELISA Kit for Rat Metalloproteinase inhibitor 1
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    E0552r
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    96T
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    ELISA Kit for Rat Metalloproteinase inhibitor 1
    MSDS:
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    ELISA Kit for Rat Metalloproteinase inhibitor 1
    Cat.:
    E0552p
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    96T
    ELISA Kit for Rat Metalloproteinase inhibitor 1
    Cat.:
    E0552b
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    96T
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    ELISA Kit for Rat Metalloproteinase inhibitor 1
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    E0552d
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    ELISA Kit for Rat Metalloproteinase inhibitor 1
    Cat.:
    E0552h
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    CLIA Kit for Rat Metalloproteinase inhibitor 1
    Cat.:
    U0552r
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    Packing:
    96T
    CLIA Kit for Rat Metalloproteinase inhibitor 1
    MSDS:
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    CLIA Kit for Rat Metalloproteinase inhibitor 1
    Cat.:
    U0552d
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    MSDS:
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    Packing:
    96T
    CLIA Kit for Rat Metalloproteinase inhibitor 1
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    U0552p
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    MSDS:
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    Packing:
    96T
    CLIA Kit for Rat Metalloproteinase inhibitor 1
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    U0552h
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    96T
    CLIA Kit for Rat Metalloproteinase inhibitor 1
    Cat.:
    U0552m
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    MSDS:
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    96T
    CLIA Kit for Rat Metalloproteinase inhibitor 1
    Cat.:
    U0552b
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    Packing:
    96T
    Polyclonal Antibody for Rat Metalloproteinase inhibitor 1
    Cat.:
    P0552Rb-m
    Price:
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat Metalloproteinase inhibitor 1
    Polyclonal Antibody for Rat Metalloproteinase inhibitor 1
    Cat.:
    P0552Rb-h
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat Metalloproteinase inhibitor 1
    Polyclonal Antibody for Rat Metalloproteinase inhibitor 1
    Polyclonal Antibody for Rat Metalloproteinase inhibitor 1
    Cat.:
    P0552Rb-r
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat Metalloproteinase inhibitor 1
    Monoclonal Antibody for Rat Metalloproteinase inhibitor 1
    Monoclonal Antibody for Rat Metalloproteinase inhibitor 1
    Monoclonal Antibody for Rat Metalloproteinase inhibitor 1
    Monoclonal Antibody for Rat Metalloproteinase inhibitor 1
    Monoclonal Antibody for Rat Metalloproteinase inhibitor 1
    Monoclonal Antibody for Rat Metalloproteinase inhibitor 1
    Monoclonal Antibody for Rat Metalloproteinase inhibitor 1
    Protein for Rat Metalloproteinase inhibitor 1
    Protein for Rat Metalloproteinase inhibitor 1
    Protein for Rat Metalloproteinase inhibitor 1
    Protein for Rat Metalloproteinase inhibitor 1
    Protein for Rat Metalloproteinase inhibitor 1
    Protein for Rat Metalloproteinase inhibitor 1
    Cat.:
    R0552h
    Price:
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    Packing:
    1mg
    Purity:
    >90%
    Protein for Rat Metalloproteinase inhibitor 1
    Cat.:
    R0552m
    Price:
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    Packing:
    1mg
    Purity:
    >90%

    R&D Technical Data
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              12% SDS-PAGE Analysis

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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤ 5.8%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤ 8.9%

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    Recovery
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    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    92-105

    Plasma

    98

    91-106

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    90-103%

    91-104%

    94-102%

    92-105%

    EDTA plasma(n=5)

    91-97%

    93-102%

    89-105%

    91-106%

    heparin plasma(n=5)

    92-104%

     

    94-108%

    95-109%

    91-107%

     

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    References
    1. 1.
      "Cloning of the cDNA encoding rat tissue inhibitor of metalloproteinase 1 (TIMP-1), amino acid comparison with other TIMPs, and gene expression in rat tissues."
      Okada A. , Garnier J.-M. , Vicaire S. , Basset P.
      Gene147:301-302(1994) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
      strain: Wistar.
      tissue: Dorsal skin.
    2. 2.
      "Analysis of the cDNA encoding rat tissue inhibitor of metalloproteinases-1."
      Gibbons K.L. , O'Grady R.L. , Piper A.A.
      Submitted (1996-01) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
      tissue: Mammary gland.
    3. 3.
      "Rat TIMP-1 mRNA sequence."
      Dai W.-J. , Jiang H.-C. , Fu S.-B.
      Submitted (2004-02) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
    4. 4.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      tissue: Prostate.
    5. 5.
      "Tissue inhibitor of metalloproteinase-1 messenger RNA expression is enhanced relative to interstitial collagenase messenger RNA in experimental liver injury and fibrosis."
      Iredale J.P. , Benyon R.C. , Arthur M.J.P. , Ferris W.F. , Alcolado R. , Winwood P.J. , Clark N. , Murphy G.
      Hepatology24:176-184(1996) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 30-205
      tissue: Liver.
    6. 6.
      "Identification of a stimulator of steroid hormone synthesis isolated from testis."
      Boujrad N. , Ogwuegbu S.O. , Garnier M. , Lee C.-H. , Martin B.M. , Papadopoulos V.
      Science268:1609-1612(1995) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 39-156;PROTEIN SEQUENCE OF 24-38;INDUCTION;FUNCTION;SUBCELLULAR LOCATION
      strain: Sprague-Dawley.
      tissue: Sertoli cell.
    7. 7.
      "Purification and sequence analysis of two rat tissue inhibitors of metalloproteinases."
      Roswit W.T. , McCourt D.W. , Partridge N.C. , Jeffrey J.J.
      Arch. Biochem. Biophys.292:402-410(1992) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 24-45;FUNCTION;SUBCELLULAR LOCATION;GLYCOSYLATION
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