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Index > Protein center > Timp4(Gene name) > Rat
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  • Timp4 (Gene name),
  • Metalloproteinase inhibitor 4 (Protein name ),  TIMP4_RAT from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • 3D
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Gene name:
    Timp4;
    Protein name:
    Metalloproteinase inhibitor 4;
    Alternative:
    Tissue inhibitor of metalloproteinases 4(TIMP-4);
    Organism:
    Rat (Rattus norvegicus). 
    General Annotation
    Sub Unit:
    N/A
    Function:
    Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor.
    Subcellular Location:
    Secreted
    Protein Attributes:
    Sequence length:
    224
    Sequence:
    50:
    MPWSPLAALS | WALVLRLLAL | LWPPGRGEAC | SCAPAHPQQH | VCHSALVIRA | 
    100:
    KISSEKVVPA | SEDPADTQKM | IRYEIKQIKM | FKGFEKAKDI | QYVYTPFDSS | 
    150:
    LCGVKLETNS | QKQYLLTGQI | LSDGKVFIHL | CNYIEPWEDL | SLVQRESLNH | 
    200:
    HYHQNCGCQI | TTCYAVPCTI | SAPDECLWTD | WLLERKLYGY | QAQHYVCMKH | 
    224:
    VDGICSWYRG | HLHLRKEYVD | IVQP
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    Pfam:
    UniGene:
    String:
    Uniprot:
     
    FOR
    ELISA Kit for Rat Metalloproteinase inhibitor 4
    Cat.:
    E0130r
    Price:
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    MSDS:
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    Packing:
    96T
    ELISA Kit for Rat Metalloproteinase inhibitor 4
    Cat.:
    E0130m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    ELISA Kit for Rat Metalloproteinase inhibitor 4
    MSDS:
    Please sign in first.
    ELISA Kit for Rat Metalloproteinase inhibitor 4
    Cat.:
    E0130b
    Price:
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    MSDS:
    Please sign in first.
    Packing:
    96T
    ELISA Kit for Rat Metalloproteinase inhibitor 4
    Cat.:
    E0130h
    Price:
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    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Rat Metalloproteinase inhibitor 4
    Cat.:
    U0130h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Rat Metalloproteinase inhibitor 4
    Cat.:
    U0130b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Rat Metalloproteinase inhibitor 4
    MSDS:
    Please sign in first.
    CLIA Kit for Rat Metalloproteinase inhibitor 4
    Cat.:
    U0130r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Rat Metalloproteinase inhibitor 4
    Cat.:
    U0130m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Rat Metalloproteinase inhibitor 4
    Polyclonal Antibody for Rat Metalloproteinase inhibitor 4
    Cat.:
    P0130Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat Metalloproteinase inhibitor 4
    Cat.:
    P0130Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat Metalloproteinase inhibitor 4
    Polyclonal Antibody for Rat Metalloproteinase inhibitor 4
    Cat.:
    P0130Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Rat Metalloproteinase inhibitor 4
    Monoclonal Antibody for Rat Metalloproteinase inhibitor 4
    Monoclonal Antibody for Rat Metalloproteinase inhibitor 4
    Monoclonal Antibody for Rat Metalloproteinase inhibitor 4
    Monoclonal Antibody for Rat Metalloproteinase inhibitor 4
    Protein for Rat Metalloproteinase inhibitor 4
    Protein for Rat Metalloproteinase inhibitor 4
    Protein for Rat Metalloproteinase inhibitor 4
    Protein for Rat Metalloproteinase inhibitor 4
    Protein for Rat Metalloproteinase inhibitor 4

    R&D Technical Data
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    s

    s
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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤8.4%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤12.6%

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

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    Recovery
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    Recovery was determined by spiking various levels of TIMP4 into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    96

    92-101

    Plasma

    97

    95-99

     

     

     

     

    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TIMP4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-99%

    93-98%

    90-102%

    93-100%

    Saliva(n=3)

    91-102%

    91-100%

    92-100%

    90-104%

    heparin plasma(n=5)

    93-99%

     

    90-103%

    94-99%

    92-102%

     

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    Saliva(n=3)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

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    References
    1. 1.
      "Genome sequence of the Brown Norway rat yields insights into mammalian evolution."
      Gibbs R.A. , Weinstock G.M. , Metzker M.L. , Muzny D.M. , Sodergren E.J. , Scherer S. , Scott G. , Steffen D. , Worley K.C. , Burch P.E. , Okwuonu G. , Hines S. , Lewis L. , Deramo C. , Delgado O. , Dugan-Rocha S. , Miner G. , Morgan M. , more...
      Nature428:493-521(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
      strain: Brown Norway.
    2. 2.
      "Molecular cloning and expression analysis of the cDNA encoding rat tissue inhibitor of metalloproteinase-4."
      Wu I. , Moses M.A.
      Matrix Biol.16:339-342(1998) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
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