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Index > Protein center > Tnf(Gene name) > Rat
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  • Tnf (Gene name),
  • Tumor necrosis factor (Protein name ),  TNFA_RAT from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Gene name:
    Tnf(Tnfa;Tnfsf2);
    Protein name:
    Tumor necrosis factor;
    Alternative:
    Tumor necrosis factor ligand superfamily member 2(TNF-a);Cachectin;TNF-alpha;
    Organism:
    Rat (Rattus norvegicus). 
    General Annotation
    Sub Unit:
    Homotrimer.
    Function:
    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
    Subcellular Location:
    Tumor necrosis factor, soluble form Secreted
    Protein Attributes:
    Sequence length:
    235
    Sequence:
    50:
    MSTESMIRDV | ELAEEALPKK | MGGLQNSRRC | LCLSLFSFLL | VAGATTLFCL | 
    100:
    LNFGVIGPNK | EEKFPNGLPL | ISSMAQTLTL | RSSSQNSSDK | PVAHVVANHQ | 
    150:
    AEEQLEWLSQ | RANALLANGM | DLKDNQLVVP | ADGLYLIYSQ | VLFKGQGCPD | 
    200:
    YVLLTHTVSR | FAISYQEKVS | LLSAIKSPCP | KDTPEGAELK | PWYEPMYLGG | 
    235:
    VFQLEKGDLL | SAEVNLPKYL | DITESGQVYF | GVIAL
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    String:
    Pfam:
    KEGG:
    UniGene:
    SMR:
    Uniprot:
     
    FOR
    ELISA Kit for Rat Tumor necrosis factor
    Cat.:
    E0133h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Rat Tumor necrosis factor
    Cat.:
    E0133d
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Rat Tumor necrosis factor
    Cat.:
    E0133p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Rat Tumor necrosis factor
    MSDS:
    Please sign in first.
    ELISA Kit for Rat Tumor necrosis factor
    Cat.:
    E0133m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Rat Tumor necrosis factor
    Cat.:
    E0133r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Rat Tumor necrosis factor
    Cat.:
    E0133b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Rat Tumor necrosis factor
    Cat.:
    U0133b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Rat Tumor necrosis factor
    Cat.:
    U0133p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Rat Tumor necrosis factor
    Cat.:
    U0133h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Rat Tumor necrosis factor
    Cat.:
    U0133d
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Rat Tumor necrosis factor
    Cat.:
    U0133m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Rat Tumor necrosis factor
    MSDS:
    Please sign in first.
    CLIA Kit for Rat Tumor necrosis factor
    Cat.:
    U0133r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Rat Tumor necrosis factor
    Cat.:
    P0133Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat Tumor necrosis factor
    Polyclonal Antibody for Rat Tumor necrosis factor
    Polyclonal Antibody for Rat Tumor necrosis factor
    Polyclonal Antibody for Rat Tumor necrosis factor
    Cat.:
    P0133Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat Tumor necrosis factor
    Polyclonal Antibody for Rat Tumor necrosis factor
    Cat.:
    P0133Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Rat Tumor necrosis factor
    Monoclonal Antibody for Rat Tumor necrosis factor
    Monoclonal Antibody for Rat Tumor necrosis factor
    Monoclonal Antibody for Rat Tumor necrosis factor
    Monoclonal Antibody for Rat Tumor necrosis factor
    Monoclonal Antibody for Rat Tumor necrosis factor
    Monoclonal Antibody for Rat Tumor necrosis factor
    Protein for Rat Tumor necrosis factor
    Protein for Rat Tumor necrosis factor
    Protein for Rat Tumor necrosis factor
    Protein for Rat Tumor necrosis factor
    Protein for Rat Tumor necrosis factor
    Cat.:
    R0133h
    Price:
    Please sign in first.
    Packing:
    1mg
    Purity:
    >90%
    Protein for Rat Tumor necrosis factor
    Cat.:
    R0133m
    Price:
    Please sign in first.
    Packing:
    1mg
    Purity:
    >90%
    Protein for Rat Tumor necrosis factor
    Cat.:
    R0133r
    Price:
    Please sign in first.
    Packing:
    1mg
    Purity:
    >90%

    R&D Technical Data
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    Precision

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤5.2%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.4%

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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤8.0%

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    Recovery

    Recovery was determined by spiking various levels of TNF into serum and plasma .

    Sample Type

    Average(%)

    Recovery  Range (%)

    Serum

    102

    95-114

    Plasma

    96

    93-99

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    Recovery was determined by spiking various levels of TNF into serum and plasma .

    Sample Type

    Average(%)

    Recovery  Range (%)

    Serum

    89

    86-104

    Plasma

    95

    86-105

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    Linearity

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    96-101%

    96-105%

    98-104%

    98-107%

    EDTA plasma(n=5)

    95-103%

    97-107%

    100-110%

    88-103%

    heparin plasma(n=5)

    98-103%

     

    96-102%

    93-104%

    95-115%

     

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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-103%

    90-101%

    98-109%

    97-110%

    EDTA plasma(n=5)

    101-105%

    97-109%

    89-107%

    99-108%

    heparin plasma(n=5)

    98-107%

     

    96-110%

    100-112%

    101-116%

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    Citations
    [2].
    Jing Tang, Xi Chen, Weifeng Tu, Yuanbo Guo, Zhenlong Zhao, Qiong Xue, Chunshui Lin, Jinfang Xiao, Xuegang Sun, Tao Tao, Miaoning Gu, Youtan Liu
    [3].
    Hatem M Abuohashish,Salim S Al-Rejaie ,Khaled A Al-Hosaini,Mihir Y Parmar,Mohammed M Ahmed
    References
    1. 1.
      "Cloning and expression in Escherichia coli of the gene for rat tumor necrosis factor."
      Shirai T. , Shimizu N. , Horiguchi S. , Ito H.
      Agric. Biol. Chem.53:1733-1736(1989)
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    2. 2.
      "Rat tumor necrosis factor-alpha. Transcription in rat Kupffer cells and in vitro posttranslational processing based on a PCR-derived cDNA."
      Estler H.C. , Grewe M. , Gaussling R. , Pavlovic M. , Decker K.F.
      Biol. Chem. Hoppe-Seyler373:271-281(1992) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
    3. 3.
      "Cloning and sequence analysis of the rat tumor necrosis factor-encoding genes."
      Kwon J. , Chung I.Y. , Benveniste E.N.
      Gene132:227-236(1993) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
      strain: Sprague-Dawley.
      tissue: Testis.
    4. 4.
      "Polymorphisms of the tumor necrosis factor alpha locus among autoimmune disease susceptible and resistant inbred rat strains."
      Furuya T. , Joe B. , Salstrom J.L. , Hashiramoto A. , Dobbins D.E. , Wilder R.L. , Remmers E.F.
      Genes Immun.2:229-232(2001) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
      strain: ACI/SegHsd.
      strain: BB(DR)/Wor.
      strain: Brown Norway/SsNHsd.
      strain: DA/Bkl.
      strain: F344/NHsd.
      strain: LEW/NHsd.
    5. 5.
      "TNF-alpha polymorphism in rats with collagen-induced arthritis."
      Seidel M.F. , Junier M.-P. , Vetter H.
      Submitted (2000-05) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];VARIANTS PRO-122 AND GLU-190
      strain: Dark agouti.
    6. 6.
      "No association between tumor necrosis factor-alpha production and gene polymorphisms among inbred rat strains."
      Warle M.C. , van der Laan L.J. , Kusters J.G. , Pot R.G. , Hop W.C. , Segeren K.C. , Ijzermans J.N. , Metselaar H.J. , Tilanus H.W.
      Transpl. Immunol.14:77-82(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
      strain: AUG/OlaHsd.
      strain: PVG/OlaHsd.
      strain: WF/HanHsd.
    7. 7.
      "The genomic sequence and comparative analysis of the rat major histocompatibility complex."
      Hurt P. , Walter L. , Sudbrak R. , Klages S. , Mueller I. , Shiina T. , Inoko H. , Lehrach H. , Guenther E. , Reinhardt R. , Himmelbauer H.
      Genome Res.14:631-639(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
      strain: Brown Norway.
    8. 8.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      tissue: Prostate.
    9. 9.
      "Mapping of the TNF-alpha locus in the rat."
      Kirisits M.J. , Vardimon D. , Kunz H.W. , Gill T.J. III
      Submitted (1993-06) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-231
      tissue: Tail.
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