TOP2B ELISA Kit for Human(Homo sapiens) | EIAab.comTOP2B ELISA Kit for Human(Homo sapiens)

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TOP2B (Gene name),

DNA topoisomerase 2-beta (Protein name ), TOP2B_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

TOP2B;

Protein name:

DNA topoisomerase 2-beta;

Alternative:

DNA topoisomerase II, beta isozyme;

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Homodimer. Component of the WINAC complex, at least composed of SMARCA2, SMARCA4, SMARCB1, SMARCC1, SMARCC2, SMARCD1, SMARCE1, ACTL6A, BAZ1B/WSTF, ARID1A, SUPT16H, CHAF1A and TOP2B.

Function:

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Indirectly ivolved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.

Subcellular Location:

Cytoplasm Nucleus nucleolus

Protein Attributes:

Sequence length:

1626

Sequence:

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1400:

1450:

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1550:

1600:

1626:

GRARKEVKYF | AESDEEEDDV | DFAMFN

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

KEGG:

hsa:7155

STRING:

9606.ENSP00000396704

MIM:

126431

Pfam:

PF00204

String:

Q02880

SMR:

Q02880

UniGene:

Hs.475733

Uniprot:

Q02880

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ELISA Kit for Human DNA topoisomerase 2-beta

Cat.:

E1188h

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96T

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ELISA Kit for Human DNA topoisomerase 2-beta

Cat.:

E1188c

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96T

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ELISA Kit for Human DNA topoisomerase 2-beta

Cat.:

E1188m

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96T

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CLIA Kit for Human DNA topoisomerase 2-beta

Cat.:

U1188h

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Manual:

Looking for manual.

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Packing:

96T

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CLIA Kit for Human DNA topoisomerase 2-beta

Cat.:

U1188c

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MSDS:

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Packing:

96T

CLIA Kit for Human DNA topoisomerase 2-beta

Cat.:

U1188m

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Packing:

96T

Polyclonal Antibody for Human DNA topoisomerase 2-beta

Cat.:

P1188Rb-m

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human DNA topoisomerase 2-beta

Cat.:

P1188Rb-h

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Packing:

40ug/0.2ml

Monoclonal Antibody for Human DNA topoisomerase 2-beta

Protein for Human DNA topoisomerase 2-beta

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

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References

1.

"Isolation of cDNA clones encoding the beta isozyme of human DNA topoisomerase II and localisation of the gene to chromosome 3p24."
Jenkins J.R. , Ayton P. , Jones T. , Davies S.L. , Simmons D.L. , Harris A.L. , Sheer D. , Hickson I.D.
Nucleic Acids Res.20:5587-5592(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
2.

"Novel HeLa topoisomerase II is the II beta isoform: complete coding sequence and homology with other type II topoisomerases."
Austin C.A. , Sng J.H. , Patel S. , Fisher L.M.
Biochim. Biophys. Acta1172:283-291(1993) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
3.

"Characterization and immunological identification of cDNA clones encoding two human DNA topoisomerase II isozymes."
Chung T.D. , Drake F.H. , Tan K.B. , Per S.R. , Crooke S.T. , Mirabelli C.K.
Proc. Natl. Acad. Sci. U.S.A.86:9431-9435(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 149-1034
4.

"Molecular cloning and characterization of the human topoisomerase IIalpha and IIbeta genes: evidence for isoform evolution through gene duplication."
Sng J.H. , Heaton V.J. , Bell M. , Mani P. , Austin C.A. , Fisher L.M.
Biochim. Biophys. Acta1444:395-406(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 910-1626
5.

"Isolation and characterization of a human cDNA clone encoding a novel DNA topoisomerase II homologue from HeLa cells."
Austin C.A. , Fisher L.M.
FEBS Lett.266:115-117(1990) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE OF 1038-1271
6.

"Binding of wild-type p53 by topoisomerase II and overexpression of topoisomerase II in human hepatocellular carcinoma."
Yuwen H. , Hsia C.C. , Nakashima Y. , Evangelista A. , Tabor E.
Biochem. Biophys. Res. Commun.234:194-197(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE OF 1277-1626
7.

"Human cells express two differentially spliced forms of topoisomerase II beta mRNA."
Davies S.L. , Jenkins J.R. , Hickson I.D.
Nucleic Acids Res.21:3719-3723(1993) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ALTERNATIVE SPLICING
8.

"Mutagenesis of E477 or K505 in the B' domain of human topoisomerase II beta increases the requirement for magnesium ions during strand passage."
West K.L. , Meczes E.L. , Thorn R. , Turnbull R.M. , Marshall R. , Austin C.A.
Biochemistry39:1223-1233(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: COFACTOR;FUNCTION;CATALYTIC ACTIVITY;MUTAGENESIS OF GLU-482; SER-485; ARG-508; LYS-510 AND ARG-515
9.

"Identification of functional nuclear export sequences in human topoisomerase IIalpha and beta."
Mirski S.E. , Bielawski J.C. , Cole S.P.
Biochem. Biophys. Res. Commun.306:905-911(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEAR EXPORT SIGNAL
10.

"The chromatin-remodeling complex WINAC targets a nuclear receptor to promoters and is impaired in Williams syndrome."
Kitagawa H. , Fujiki R. , Yoshimura K. , Mezaki Y. , Uematsu Y. , Matsui D. , Ogawa S. , Unno K. , Okubo M. , Tokita A. , Nakagawa T. , Ito T. , Ishimi Y. , Nagasawa H. , Matsumoto T. , Yanagisawa J. , Kato S.
Cell113:905-917(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY;IDENTIFICATION IN THE WINAC COMPLEX;FUNCTION
11.

"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V. , Blagoev B. , Gnad F. , Macek B. , Kumar C. , Mortensen P. , Mann M.
Cell127:635-648(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1336; SER-1340; SER-1400 AND SER-1413;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
12.

"A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
Beausoleil S.A. , Villen J. , Gerber S.A. , Rush J. , Gygi S.P.
Nat. Biotechnol.24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
13.

"Toward a global characterization of the phosphoproteome in prostate cancer cells: identification of phosphoproteins in the LNCaP cell line."
Giorgianni F. , Zhao Y. , Desiderio D.M. , Beranova-Giorgianni S.
Electrophoresis28:2027-2034(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Prostate cancer.
14.

"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."
Matsuoka S. , Ballif B.A. , Smogorzewska A. , McDonald E.R. III , Hurov K.E. , Luo J. , Bakalarski C.E. , Zhao Z. , Solimini N. , Lerenthal Y. , Shiloh Y. , Gygi S.P. , Elledge S.J.
Science316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1454;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Embryonic kidney.
15.

"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
Cantin G.T. , Yi W. , Lu B. , Park S.K. , Xu T. , Lee J.-D. , Yates J.R. III
J. Proteome Res.7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1400; SER-1413 AND SER-1581;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
16.

"Phosphorylation analysis of primary human T lymphocytes using sequential IMAC and titanium oxide enrichment."
Carrascal M. , Ovelleiro D. , Casas V. , Gay M. , Abian J.
J. Proteome Res.7:5167-5176(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: T-cell.
17.

"A quantitative atlas of mitotic phosphorylation."
Dephoure N. , Zhou C. , Villen J. , Beausoleil S.A. , Bakalarski C.E. , Elledge S.J. , Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A.105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1336; SER-1340; SER-1342; SER-1344; SER-1375; SER-1400; SER-1413; TYR-1421; SER-1424; SER-1466; SER-1550; SER-1552; SER-1581; THR-1592; SER-1596; TYR-1609 AND SER-1613;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
18.

"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S. , Helbig A.O. , Slijper M. , Krijgsveld J. , Heck A.J. , Mohammed S.
Anal. Chem.81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
19.

"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V. , Lundgren D.H. , Hwang S.-I. , Rezaul K. , Wu L. , Eng J.K. , Rodionov V. , Han D.K.
Sci. Signal.2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1236; THR-1292; SER-1342; SER-1344; SER-1375; SER-1400; THR-1403; SER-1413; SER-1424; SER-1461; SER-1466; SER-1473; SER-1476; SER-1522; SER-1524; SER-1526; THR-1575 AND SER-1581;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Leukemic T-cell.
20.

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V. , Vermeulen M. , Santamaria A. , Kumar C. , Miller M.L. , Jensen L.J. , Gnad F. , Cox J. , Jensen T.S. , Nigg E.A. , Brunak S. , Mann M.
Sci. Signal.3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1236; SER-1336; SER-1340; SER-1342; SER-1344; SER-1400; SER-1413; SER-1424; SER-1441; SER-1452; SER-1454; SER-1466; SER-1476; SER-1522; SER-1524; SER-1526 AND SER-1613;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
21.

"Initial characterization of the human central proteome."
Burkard T.R. , Planyavsky M. , Kaupe I. , Breitwieser F.P. , Buerckstuemmer T. , Bennett K.L. , Superti-Furga G. , Colinge J.
BMC Syst. Biol.5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
22.

"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T. , Prokhorova T.A. , Akimov V. , Henningsen J. , Johansen P.T. , Kratchmarova I. , Kassem M. , Mann M. , Olsen J.V. , Blagoev B.
Sci. Signal.4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1336; SER-1340; SER-1342; SER-1344; SER-1358; SER-1400; SER-1413; SER-1424; SER-1441; SER-1454; SER-1466; SER-1522; SER-1524; SER-1550; SER-1581 AND SER-1613;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
23.

"Structural basis of type II topoisomerase inhibition by the anticancer drug etoposide."
Wu C.C. , Li T.K. , Farh L. , Lin L.Y. , Lin T.S. , Yu Y.J. , Yen T.J. , Chiang C.W. , Chan N.L.
Science333:459-462(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.16 ANGSTROMS) OF 450-1206 IN COMPLEX WITH DNA; MAGNESIUM AND ETOPOSIDE;ACTIVE SITE;COFACTOR;SUBUNIT

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