TPH1 ELISA Kit for Human(Homo sapiens) | EIAab.comTPH1 ELISA Kit for Human(Homo sapiens)

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Index > Protein center > TPH1(Gene name) > Human

TPH1 (Gene name),

Tryptophan 5-hydroxylase 1 (Protein name ), TPH1_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

TPH1(TPH;TPRH;TRPH);

Protein name:

Tryptophan 5-hydroxylase 1;

Alternative:

Tryptophan 5-monooxygenase 1;

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Homotetramer.

Function:

N/A

Subcellular Location:

N/A

Protein Attributes:

Sequence length:

444

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

444:

YNPYTRSIQI | LKDTKSITSA | MNELQHDLDV | VSDALAKVSR | KPSI

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

KEGG:

hsa:7166

SMR:

P17752

STRING:

9606.ENSP00000250018

UniGene:

Hs.591999

Pfam:

PF01842

MIM:

191060

String:

P17752

Uniprot:

P17752

FOR

ELISA Kit for Human Tryptophan 5-hydroxylase 1

Cat.:

E2230h

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96T

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ELISA Kit for Human Tryptophan 5-hydroxylase 1

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ELISA Kit for Human Tryptophan 5-hydroxylase 1

Cat.:

E2230r

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96T

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ELISA Kit for Human Tryptophan 5-hydroxylase 1

Cat.:

E2230c

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96T

ELISA Kit for Human Tryptophan 5-hydroxylase 1

Cat.:

E2230m

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96T

CLIA Kit for Human Tryptophan 5-hydroxylase 1

Cat.:

U2230h

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96T

CLIA Kit for Human Tryptophan 5-hydroxylase 1

Cat.:

U2230m

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MSDS:

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Packing:

96T

CLIA Kit for Human Tryptophan 5-hydroxylase 1

Cat.:

U2230r

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Manual:

Looking for manual.

MSDS:

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Packing:

96T

Range:

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CLIA Kit for Human Tryptophan 5-hydroxylase 1

MSDS:

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CLIA Kit for Human Tryptophan 5-hydroxylase 1

Cat.:

U2230c

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Packing:

96T

Polyclonal Antibody for Human Tryptophan 5-hydroxylase 1

Cat.:

P2230Rb-m

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human Tryptophan 5-hydroxylase 1

Cat.:

P2230Rb-r

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40ug/0.2ml

Polyclonal Antibody for Human Tryptophan 5-hydroxylase 1

Cat.:

P2230Rb-h

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Packing:

40ug/0.2ml

Monoclonal Antibody for Human Tryptophan 5-hydroxylase 1

Protein for Human Tryptophan 5-hydroxylase 1

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤10.8%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

Recovery was determined by spiking various levels of TPH1 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	92-104
Plasma	98	93-105

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TPH1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-109%	95-104%	93-107%
EDTA plasma(n=5)	91-103%	93-105%	90-107%	95-102%
heparin plasma(n=5)	93-104%	94-102%	92-104%	91-107%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Complete coding sequence of human tryptophan hydroxylase."
Boulalard S. , Darmon M.C. , Ganem Y. , Launay J.-M. , Mallet J.
Nucleic Acids Res.18:4257-4257(1990) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
tissue: Carcinoma.
2.

"Cloning and expression of rabbit and human brain tryptophan hydroxylase cDNA in Escherichia coli."
Tipper J.P. , Citron B.A. , Ribeiro P. , Kaufman S.
Arch. Biochem. Biophys.315:445-453(1994) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
tissue: Brain.
3.

Mural R.J. , Istrail S. , Sutton G.G. , Florea L. , Halpern A.L. , Mobarry C.M. , Lippert R. , Walenz B. , Shatkay H. , Dew I. , Miller J.R. , Flanigan M.J. , Edwards N.J. , Bolanos R. , Fasulo D. , Halldorsson B.V. , Hannenhalli S. , Turner R. , more...
Submitted (2005-09) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
4.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
5.

"Alternative splicing at the 3'-cDNA of human tryptophan hydroxylase."
Wang G.A. , Coon S.L. , Kaufman S.
J. Neurochem.71:1769-1772(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 388-444 (ISOFORMS 1 AND 2)
6.

"Three-dimensional structure of human tryptophan hydroxylase and its implications for the biosynthesis of the neurotransmitters serotonin and melatonin."
Wang L. , Erlandsen H. , Haavik J. , Knappskog P.M. , Stevens R.C.
Biochemistry41:12569-12574(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 102-402

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