TNFRSF11B ELISA Kit for Human(Homo sapiens) | EIAab.comTNFRSF11B ELISA Kit for Human(Homo sapiens)

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Index > Protein center > TNFRSF11B(Gene name) > Human

TNFRSF11B (Gene name),

Tumor necrosis factor receptor superfamily member 11B (Protein name ), TR11B_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

Citations (3)

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Gene name:

TNFRSF11B(OCIF;OPG);

Protein name:

Tumor necrosis factor receptor superfamily member 11B;

Alternative:

Osteoprotegerin;Osteoclastogenesis inhibitory factor;

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Homodimer.

Function:

Acts as decoy receptor for RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local RANKL/OPG ratio. May also play a role in preventing arterial calcification. May act as decoy receptor for TRAIL and protect against apoptosis. TRAIL binding blocks the inhibition of osteoclastogenesis.

Subcellular Location:

Secreted

Protein Attributes:

Sequence length:

401

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

401:

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

SMR:

O00300

MIM:

239000

String:

O00300

Pfam:

PF00531

STRING:

9606.ENSP00000297350

KEGG:

hsa:4982

UniGene:

Hs.81791

Uniprot:

O00300

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ELISA Kit for Human Tumor necrosis factor receptor superfamily member 11B

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E0108h

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96T

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ELISA Kit for Human Tumor necrosis factor receptor superfamily member 11B

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E0108r

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96T

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ELISA Kit for Human Tumor necrosis factor receptor superfamily member 11B

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E0108m

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ELISA Kit for Human Tumor necrosis factor receptor superfamily member 11B

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E0108b

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96T

CLIA Kit for Human Tumor necrosis factor receptor superfamily member 11B

Cat.:

U0108r

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96T

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CLIA Kit for Human Tumor necrosis factor receptor superfamily member 11B

Cat.:

U0108b

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Packing:

96T

CLIA Kit for Human Tumor necrosis factor receptor superfamily member 11B

Cat.:

U0108m

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Packing:

96T

CLIA Kit for Human Tumor necrosis factor receptor superfamily member 11B

Cat.:

U0108h

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96T

Polyclonal Antibody for Human Tumor necrosis factor receptor superfamily member 11B

Cat.:

P0108Rb-r

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40ug/0.2ml

Polyclonal Antibody for Human Tumor necrosis factor receptor superfamily member 11B

Cat.:

P0108Rb-m

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human Tumor necrosis factor receptor superfamily member 11B

Cat.:

P0108Rb-h

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human Tumor necrosis factor receptor superfamily member 11B

Monoclonal Antibody for Human Tumor necrosis factor receptor superfamily member 11B

Protein for Human Tumor necrosis factor receptor superfamily member 11B

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.1%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.9%

Intra-Assay CV: ≤2.3

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of TR11B into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	92-98
Plasma	98	95-102

Inter-Assay CV: ≤7.5

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TR11B and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	93-100%	93-99%	95-102%	93-98%
EDTA plasma(n=5)	90-96%	95-102%	101-105%	94-103%
heparin plasma(n=5)	94-99%	96-104%	95-100%	92-104%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Citations

[1].

"The Inhibitory Effect of Roasted Licorice Extract on Human Metastatic Breast Cancer Cell-Induced Bone Destruction"

Sun Kyoung Lee, Kwang-Kyun Park, Jung Han Yoon Park, Soon Sung Lim, Won-Yoon Chung

From:PhytotherapyReseach

[2].

"The Inhibitory Effect of Roasted Licorice Extract on Human Metastatic Breast Cancer Cell‐Induced Bone Destruction"

SK Lee, KK Park, JHY Park, et al.

From:Phytotherapy Research

[3].

"Isoliquiritigenin inhibits metastatic breast cancer cell-induced receptor activator of nuclear factor kappa-B ligand/osteoprotegerin ratio in human..."

Lee S K, Park K K, Kim K R, et al.

From:Journal of cancer prevention

References

1.

"Osteoprotegerin: a novel secreted protein involved in the regulation of bone density."
Simonet W.S. , Lacey D.L. , Dunstan C.R. , Kelley M. , Chang M.-S. , Luethy R. , Nguyen H.Q. , Wooden S. , Bennett L. , Boone T. , Shimamoto G. , Derose M. , Elliott R. , Colombero A. , Tan H.-L. , Trail G. , Sullivan J. , Davy E. , more...
Cell89:309-319(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];VARIANT LYS-3
tissue: Kidney.
2.

"Identity of osteoclastogenesis inhibitory factor (OCIF) and osteoprotegerin (OPG): a mechanism by which OPG/OCIF inhibits osteoclastogenesis in vitro."
Yasuda H. , Shima N. , Nakagawa N. , Mochizuki S. , Yano K. , Fujise N. , Sato Y. , Goto M. , Yamaguchi K. , Kuriyama M. , Kanno T. , Murakami A. , Tsuda E. , Morinaga T. , Higashio K.
Endocrinology139:1329-1337(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];VARIANT LYS-3
tissue: Lung cancer.
3.

"Cloning and characterization of the gene encoding human osteoprotegerin/osteoclastogenesis-inhibitory factor."
Morinaga T. , Nakagawa N. , Yasuda H. , Tsuda E. , Higashio K.
Eur. J. Biochem.254:685-691(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANT LYS-3
tissue: Placenta.
4.

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA];VARIANT LYS-3
tissue: Kidney.
5.

Suzuki Y. , Sugano S. , Totoki Y. , Toyoda A. , Takeda T. , Sakaki Y. , Tanaka A. , Yokoyama S.
Submitted (2005-04) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
tissue: Lung.
6.

NIEHS SNPs program
Submitted (2003-11) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANTS LYS-3 AND MET-104
7.

"DNA sequence and analysis of human chromosome 8."
Nusbaum C. , Mikkelsen T.S. , Zody M.C. , Asakawa S. , Taudien S. , Garber M. , Kodira C.D. , Schueler M.G. , Shimizu A. , Whittaker C.A. , Chang J.L. , Cuomo C.A. , Dewar K. , FitzGerald M.G. , Yang X. , Allen N.R. , Anderson S. , Asakawa T. , more...
Nature439:331-335(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
8.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
tissue: Eye.
9.

"Characterization of monomeric and homodimeric forms of osteoclastogenesis inhibitory factor."
Tomoyasu A. , Goto M. , Fujise N. , Mochizuki S. , Yasuda H. , Morinaga T. , Tsuda E. , Higashio K.
Biochem. Biophys. Res. Commun.245:382-387(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 22-36 AND 378-401
10.

"Signal peptide prediction based on analysis of experimentally verified cleavage sites."
Zhang Z. , Henzel W.J.
Protein Sci.13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 22-36
11.

"Cloning and expression of a novel mutated osteoprogerin/osteoclastogenesis inhibitory factor gene."
He Z.-Y. , Yang G.-Z. , Zhang W.-J. , Wu X.-F.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao31:680-684(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 22-393
tissue: Placenta.
12.

"Isolation of a novel cytokine from human fibroblasts that specifically inhibits osteoclastogenesis."
Tsuda E. , Goto M. , Mochizuki S. , Yano K. , Kobayashi F. , Morinaga T. , Higashio K.
Biochem. Biophys. Res. Commun.234:137-142(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 242-255; 354-359 AND 369-378;FUNCTION
13.

"Osteoprotegerin is a receptor for the cytotoxic ligand TRAIL."
Emery J.G. , McDonnell P. , Burke M.B. , Deen K.C. , Lyn S. , Silverman C. , Dul E. , Appelbaum E.R. , Eichman C. , DiPrinzio R. , Dodds R.A. , James I.E. , Rosenberg M. , Lee J.C. , Young P.R.
J. Biol. Chem.273:14363-14367(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH TNFSF10
14.

"Characterization of structural domains of human osteoclastogenesis inhibitory factor."
Yamaguchi K. , Kinosaki M. , Goto M. , Kobayashi F. , Tsuda E. , Morinaga T. , Higashio K.
J. Biol. Chem.273:5117-5123(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: CHARACTERIZATION;MUTAGENESIS OF CYS-400
15.

"Receptor activator of nuclear factor-kappaB ligand and osteoprotegerin: potential implications for the pathogenesis and treatment of malignant bone diseases."
Hofbauer L.C. , Neubauer A. , Heufelder A.E.
Cancer92:460-470(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW
16.

"Crystal structure of human RANKL complexed with its decoy receptor osteoprotegerin."
Luan X. , Lu Q. , Jiang Y. , Zhang S. , Wang Q. , Yuan H. , Zhao W. , Wang J. , Wang X.
J. Immunol.189:245-252(2012) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 22-186 IN COMPLEX WITH TNFSF11;INTERACTION WITH TNFSF11;SUBUNIT;FUNCTION;MUTAGENESIS OF 78-ASP-GLU-79 AND GLU-116;GLYCOSYLATION AT ASN-178;DISULFIDE BONDS
17.

"A mutation in the gene TNFRSF11B encoding osteoprotegerin causes an idiopathic hyperphosphatasia phenotype."
Cundy T. , Hegde M. , Naot D. , Chong B. , King A. , Wallace R. , Mulley J. , Love D.R. , Seidel J. , Fawkner M. , Banovic T. , Callon K.E. , Grey A.B. , Reid I.R. , Middleton-Hardie C.A. , Cornish J.
Hum. Mol. Genet.11:2119-2127(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT JPD ASP-182 DEL

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