Gene name:
UBE3A (E6AP;EPVE6AP;HPVE6A) ;
Protein name:
Ubiquitin-protein ligase E3A ;
Alternative:
Human papillomavirus E6-associated protein ;E6AP ubiquitin-protein ligase ;Renal carcinoma antigen NY-REN-54 ;Oncogenic protein-associated protein E6-AP ;
Organism:
Human (Homo sapiens).
General Annotation
Sub Unit:
Binds UBQLN1 and UBQLN2. Interacts with the 26S proteasome. Interacts with HCV core protein and targets it to degradation. Interacts with the E6 protein of the cancer-associated human papillomavirus types 16 and 18. The E6/E6-AP complex binds to and targets the p53/TP53 tumor-suppressor protein for ubiquitin-mediated proteolysis. Interacts with BPY2.
Function:
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. Additionally, may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. Finally, UBE3A also promotes its own degradation in vivo.
Subcellular Location:
Nucleus
Protein Attributes:
50:
MEKLHQCYWK | SGEPQSDDIE | ASRMKRAAAK | HLIERYYHQL | TEGCGNEACT |
100:
NEFCASCPTF | LRMDNNAAAI | KALELYKINA | KLCDPHPSKK | GASSAYLENS |
150:
KGAPNNSCSE | IKMNKKGARI | DFKDVTYLTE | EKVYEILELC | REREDYSPLI |
200:
RVIGRVFSSA | EALVQSFRKV | KQHTKEELKS | LQAKDEDKDE | DEKEKAACSA |
250:
AAMEEDSEAS | SSRIGDSSQG | DNNLQKLGPD | DVSVDIDAIR | RVYTRLLSNE |
300:
KIETAFLNAL | VYLSPNVECD | LTYHNVYSRD | PNYLNLFIIV | MENRNLHSPE |
350:
YLEMALPLFC | KAMSKLPLAA | QGKLIRLWSK | YNADQIRRMM | ETFQQLITYK |
400:
VISNEFNSRN | LVNDDDAIVA | ASKCLKMVYY | ANVVGGEVDT | NHNEEDDEEP |
450:
IPESSELTLQ | ELLGEERRNK | KGPRVDPLET | ELGVKTLDCR | KPLIPFEEFI |
500:
NEPLNEVLEM | DKDYTFFKVE | TENKFSFMTC | PFILNAVTKN | LGLYYDNRIR |
550:
MYSERRITVL | YSLVQGQQLN | PYLRLKVRRD | HIIDDALVRL | EMIAMENPAD |
600:
LKKQLYVEFE | GEQGVDEGGV | SKEFFQLVVE | EIFNPDIGMF | TYDESTKLFW |
650:
FNPSSFETEG | QFTLIGIVLG | LAIYNNCILD | VHFPMVVYRK | LMGKKGTFRD |
700:
LGDSHPVLYQ | SLKDLLEYEG | NVEDDMMITF | QISQTDLFGN | PMMYDLKENG |
750:
DKIPITNENR | KEFVNLYSDY | ILNKSVEKQF | KAFRRGFHMV | TNESPLKYLF |
800:
RPEEIELLIC | GSRNLDFQAL | EETTEYDGGY | TRDSVLIREF | WEIVHSFTDE |
850:
QKRLFLQFTT | GTDRAPVGGL | GKLKMIIAKN | GPDTERLPTS | HTCFNVLLLP |
875:
EYSSKEKLKE | RLLKAITYAK | GFGML
Vaild Sequence:
Related Databases
Uniprot:
ELISA Kit
CLIA Kit
Polyclonal Antibody
Monoclonal Antibody
Protein
FOR
Human
Mouse
ELISA Kit for Human Ubiquitin-protein ligase E3A
ELISA Kit for Human Ubiquitin-protein ligase E3A
CLIA Kit for Human Ubiquitin-protein ligase E3A
CLIA Kit for Human Ubiquitin-protein ligase E3A
Polyclonal Antibody for Human Ubiquitin-protein ligase E3A
Polyclonal Antibody for Human Ubiquitin-protein ligase E3A
Monoclonal Antibody for Human Ubiquitin-protein ligase E3A
Monoclonal Antibody for Human Ubiquitin-protein ligase E3A
Protein for Human Ubiquitin-protein ligase E3A
Protein for Human Ubiquitin-protein ligase E3A
R&D Technical Data
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Precision
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Recovery
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Linearity
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Citations
[1].
YC Lee, HP Wang, CP Wang, et al
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[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
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[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
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tissue :
Keratinocyte .
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[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : CHARACTERIZATION
9.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
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[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
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tissue :
Renal cell carcinoma .
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[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
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12.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : INTERACTION WITH UBQLN1 AND UBQLN2
13.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : INTERACTION WITH BPY2
14.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue :
Embryonic kidney .
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[
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[
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[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : INTERACTION WITH HCV CORE PROTEIN
16.
"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."
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Science316:1160-1166(2007)
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[
Europe PMC ]
[
Abstract ]
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-218;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue :
Embryonic kidney .
17.
"E6AP promotes the degradation of the PML tumor suppressor."
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Europe PMC ]
[
Abstract ]
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : FUNCTION
18.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : FUNCTION
19.
"Identification of annexin A1 as a novel substrate for E6AP-mediated ubiquitylation."
Shimoji T.
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Murakami K.
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Sugiyama Y.
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Matsuda M.
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Inubushi S.
,
Nasu J.
,
Shirakura M.
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Hotta H.
,
Miyamura T.
,
Shoji I.
J. Cell. Biochem.106:1123-1135(2009)
[
PubMed ]
[
Europe PMC ]
[
Abstract ]
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : FUNCTION
20.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : FUNCTION
21.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-218;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue :
Leukemic T-cell .
22.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
23.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : FUNCTION;INTERACTION WITH HIF1AN; MAPK6; NEURL4 AND PSMD4
24.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : FUNCTION;INTERACTION WITH ARNTL
25.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 495-852 IN COMPLEX WITH UBE2L3
26.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : STRUCTURE BY NMR OF 401-418
27.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : VARIANT AS ILE-826 INS;VARIANTS HIS-62; THR-201 AND PRO-372