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Index > Protein center > VASP(Gene name) > Human
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  • VASP (Gene name),
  • Vasodilator-stimulated phosphoprotein (Protein name ),  VASP_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Gene name:
    VASP;
    Protein name:
    Vasodilator-stimulated phosphoprotein(VASP);
    Alternative:

    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    Homotetramer. Interacts with PFN1, PFN2, LPP, ACTN1 and ACTG1. Interacts, via the EVH1 domain, with the Pro-rich regions of ZYX. This interaction is important for targeting to focal adhesions and the formation of actin-rich structures at the apical surface of cells. Interacts, via the EVH1 domain, with the Pro-rich domain of Listeria monocytogenes actA. Interacts with APBB1IP. Interacts, via the Pro-rich domain, with the C-terminal SH3 domain of DNMBP.
    Function:
    Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.
    Subcellular Location:
    Cytoplasm Cytoplasm cytoskeleton Cell junction focal adhesion Cell projection lamellipodium membrane Cell projection filopodium membrane Targeted to stress fibers and focal adhesions through interaction with a number of proteins including MRL family members. Localizes to the plasma membrane in protruding lamellipodia and filopodial tips. Stimulation by thrombin or PMA, also translocates VASP to focal adhesions. Localized along the sides of actin filaments throughout the peripheral cytoplasm under basal conditions.
    Protein Attributes:
    Sequence length:
    380
    Sequence:
    50:
    MSETVICSSR | ATVMLYDDGN | KRWLPAGTGP | QAFSRVQIYH | NPTANSFRVV | 
    100:
    GRKMQPDQQV | VINCAIVRGV | KYNQATPNFH | QWRDARQVWG | LNFGSKEDAA | 
    150:
    QFAAGMASAL | EALEGGGPPP | PPALPTWSVP | NGPSPEEVEQ | QKRQQPGPSE | 
    200:
    HIERRVSNAG | GPPAPPAGGP | PPPPGPPPPP | GPPPPPGLPP | SGVPAAAHGA | 
    250:
    GGGPPPAPPL | PAAQGPGGGG | AGAPGLAAAI | AGAKLRKVSK | QEEASGGPTA | 
    300:
    PKAESGRSGG | GGLMEEMNAM | LARRRKATQV | GEKTPKDESA | NQEEPEARVP | 
    350:
    AQSESVRRPW | EKNSTTLPRM | KSSSSVTTSE | TQPCTPSSSD | YSDLQRVKQE | 
    380:
    LLEEVKKELQ | KVKEEIIEAF | VQELRKRGSP | 
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    UniGene:
    String:
    Pfam:
    Pfam:
    SMR:
    MIM:
    KEGG:
    Uniprot:
     
    FOR
    ELISA Kit for Human VASP
    Cat.:
    E9578h
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    Packing:
    96T
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    ELISA Kit for Human VASP
    MSDS:
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    ELISA Kit for Human VASP
    Cat.:
    E9578b
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    MSDS:
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    Packing:
    96T
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    ELISA Kit for Human VASP
    Cat.:
    E9578m
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    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human VASP
    MSDS:
    Please sign in first.
    CLIA Kit for Human VASP
    Cat.:
    U9578h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human VASP
    Cat.:
    U9578b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human VASP
    Cat.:
    U9578m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Human VASP
    Cat.:
    P9578Rb-m
    Price:
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human VASP
    Cat.:
    P9578Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human VASP
    Polyclonal Antibody for Human VASP
    Monoclonal Antibody for Human VASP
    Monoclonal Antibody for Human VASP
    Monoclonal Antibody for Human VASP
    Monoclonal Antibody for Human VASP
    Protein for Human VASP
    Protein for Human VASP
    Protein for Human VASP
    Protein for Human VASP

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    Precision
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    Recovery
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    Linearity
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    References
    1. 1.
      "Molecular cloning, structural analysis and functional expression of the proline-rich focal adhesion and microfilament-associated protein VASP."
      Haffner C. , Jarchau T. , Reinhard M. , Hoppe J. , Lohmann S.M. , Walter U.
      EMBO J.14:19-27(1995) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];PROTEIN SEQUENCE OF 11-32; 87-96; 140-154; 255-282 AND 297-322;SUBCELLULAR LOCATION;TISSUE SPECIFICITY;POSSIBLE FUNCTION
      tissue: Promyelocyte.
    2. 2.
      "Role of proteins of the Ena/VASP family in actin-based motility of Listeria monocytogenes."
      Laurent V. , Loisel T.P. , Harbeck B. , Wehman A. , Groebe L. , Jockusch B.M. , Wehland J. , Gertler F.B. , Carlier M.-F.
      J. Cell Biol.144:1245-1258(1999) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];FUNCTION;INTERACTION WITH L.MONOCYTOGENES ACTA
    3. 3.
      "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
      Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      tissue: Substantia nigra.
    4. 4.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    5. 5.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      tissue: Fetal lung.
      tissue: Fetal spleen.
      tissue: Skin.
    6. 6.
      "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides."
      Gevaert K. , Goethals M. , Martens L. , Van Damme J. , Staes A. , Thomas G.R. , Vandekerckhove J.
      Nat. Biotechnol.21:566-569(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 2-10;ACETYLATION AT SER-2
      tissue: Platelet.
    7. 7.
      "Cloning of the VASP (vasodilator-stimulated phosphoprotein) genes in human and mouse: structure, sequence, and chromosomal localization."
      Zimmer M. , Fink T. , Fischer L. , Hauser W. , Scherer K. , Lichter P. , Walter U.
      Genomics36:227-233(1996) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 3-380
    8. 8.
      "cAMP- and cGMP-dependent protein kinase phosphorylation sites of the focal adhesion vasodilator-stimulated phosphoprotein (VASP) in vitro and in intact human platelets."
      Butt E. , Abel K. , Krieger M. , Palm D. , Hoppe V. , Hoppe J. , Walter U.
      J. Biol. Chem.269:14509-14517(1994) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 143-164; 235-244 AND 267-285;PHOSPHORYLATION AT SER-157; SER-239 AND THR-278 BY PRKG1
    9. 9.
      "Phosphorylation of focal adhesion vasodilator-stimulated phosphoprotein at Ser157 in intact human platelets correlates with fibrinogen receptor inhibition."
      Horstrup K. , Jablonka B. , Honig-Liedl P. , Just M. , Kochsiek K. , Walter U.
      Eur. J. Biochem.225:21-27(1994) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT SER-157;FIBRINOGEN RECEPTOR INHIBITION
    10. 10.
      "The proline-rich focal adhesion and microfilament protein VASP is a ligand for profilins."
      Reinhard M. , Giehl K. , Abel K. , Haffner C. , Jarchau T. , Hoppe V. , Jockusch B.M. , Walter U.
      EMBO J.14:1583-1589(1995) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH ACTG1 AND PFN1
    11. 11.
      "The EVH2 domain of the vasodilator-stimulated phosphoprotein mediates tetramerization, F-actin binding, and actin bundle formation."
      Bachmann C. , Fischer L. , Walter U. , Reinhard M.
      J. Biol. Chem.274:23549-23557(1999) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION OF EVH2 DOMAIN
    12. 12.
      "Characterization of the interaction between zyxin and members of the Ena/vasodilator-stimulated phosphoprotein family of proteins."
      Drees B. , Friederich E. , Fradelizi J. , Louvard D. , Beckerle M.C. , Golsteyn R.M.
      J. Biol. Chem.275:22503-22511(2000) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH ZYX
    13. 13.
      "LPP, an actin cytoskeleton protein related to zyxin, harbors a nuclear export signal and transcriptional activation capacity."
      Petit M.M. , Fradelizi J. , Golsteyn R.M. , Ayoubi T.A. , Menichi B. , Louvard D. , Van de Ven W.J.M. , Friederich E.
      Mol. Biol. Cell11:117-129(2000) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH LPP
    14. 14.
      "Lamellipodin, an Ena/VASP ligand, is implicated in the regulation of lamellipodial dynamics."
      Krause M. , Leslie J.D. , Stewart M. , Lafuente E.M. , Valderrama F. , Jagannathan R. , Strasser G.A. , Rubinson D.A. , Liu H. , Way M. , Yaffe M.B. , Boussiotis V.A. , Gertler F.B.
      Dev. Cell7:571-583(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH RAPH1
    15. 15.
      "Vasodilator-stimulated phosphoprotein is a substrate for protein kinase C."
      Chitaley K. , Chen L. , Galler A. , Walter U. , Daum G. , Clowes A.W.
      FEBS Lett.556:211-215(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION BY PKC
    16. 16.
      "Ena/VASP proteins enhance actin polymerization in the presence of barbed end capping proteins."
      Barzik M. , Kotova T.I. , Higgs H.N. , Hazelwood L. , Hanein D. , Gertler F.B. , Schafer D.A.
      J. Biol. Chem.280:28653-28662(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;LACK OF ACTIN NUCLEATION ACTIVITY;FUNCTION IN ACTIN FILAMENT ELONGATION
    17. 17.
      "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
      Rush J. , Moritz A. , Lee K.A. , Guo A. , Goss V.L. , Spek E.J. , Zhang H. , Zha X.-M. , Polakiewicz R.D. , Comb M.J.
      Nat. Biotechnol.23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-39;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
    18. 18.
      "Vasodilator-stimulated phosphoprotein (VASP) is phosphorylated on Ser 157 by protein kinase C-dependent and -independent mechanisms in thrombin-stimulated human platelets."
      Wentworth J.K. , Pula G. , Poole A.W.
      Biochem. J.393:555-564(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT SER-157;SUBCELLULAR LOCATION
    19. 19.
      "Unusual splicing events result in distinct Xin isoforms that associate differentially with filamin c and Mena/VASP."
      van der Ven P.F.M. , Ehler E. , Vakeel P. , Eulitz S. , Schenk J.A. , Milting H. , Micheel B. , Fuerst D.O.
      Exp. Cell Res.312:2154-2167(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH XIRP1
    20. 20.
      "AMP-activated protein kinase impairs endothelial actin cytoskeleton assembly by phosphorylating vasodilator-stimulated phosphoprotein."
      Blume C. , Benz P.M. , Walter U. , Ha J. , Kemp B.E. , Renne T.
      J. Biol. Chem.282:4601-4612(2007) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT THR-278;MUTAGENESIS OF SER-157; SER-239 AND THR-278;FUNCTION
    21. 21.
      "Carbon monoxide and nitric oxide mediate cytoskeletal reorganization in microvascular cells via vasodilator-stimulated phosphoprotein phosphorylation: evidence for blunted responsiveness in diabetes."
      Li Calzi S. , Purich D.L. , Chang K.H. , Afzal A. , Nakagawa T. , Busik J.V. , Agarwal A. , Segal M.S. , Grant M.B.
      Diabetes57:2488-2494(2008) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;SUBCELLULAR LOCATION;PHOSPHORYLATION AT SER-157 AND SER-239
    22. 22.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Platelet.
    23. 23.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-316 AND SER-322;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Cervix carcinoma.
    24. 24.
      "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S. , Helbig A.O. , Slijper M. , Krijgsveld J. , Heck A.J. , Mohammed S.
      Anal. Chem.81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
    25. 25.
      "Pyrin and ASC co-localize to cellular sites that are rich in polymerizing actin."
      Waite A.L. , Schaner P. , Hu C. , Richards N. , Balci-Peynircioglu B. , Hong A. , Fox M. , Gumucio D.L.
      Exp. Biol. Med. (Maywood)234:40-52(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION;INTERACTION WITH MEFV
    26. 26.
      "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V. , Lundgren D.H. , Hwang S.-I. , Rezaul K. , Wu L. , Eng J.K. , Rodionov V. , Han D.K.
      Sci. Signal.2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Leukemic T-cell.
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      "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
      Choudhary C. , Kumar C. , Gnad F. , Nielsen M.L. , Rehman M. , Walther T.C. , Olsen J.V. , Mann M.
      Science325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-283;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
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      Olsen J.V. , Vermeulen M. , Santamaria A. , Kumar C. , Miller M.L. , Jensen L.J. , Gnad F. , Cox J. , Jensen T.S. , Nigg E.A. , Brunak S. , Mann M.
      Sci. Signal.3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-316;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Cervix carcinoma.
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      Sci. Signal.4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
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      Bienvenut W.V. , Sumpton D. , Martinez A. , Lilla S. , Espagne C. , Meinnel T. , Giglione C.
      Mol. Cell. Proteomics11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
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      Ball L.J. , Kuehne R. , Hoffmann B. , Haefner A. , Schmieder P. , Volkmer-Engert R. , Hof M. , Wahl M. , Schneider-Mergener J. , Walter U. , Oschkinat H. , Jarchau T.
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      "The VASP tetramerization domain is a right-handed coiled coil based on a 15-residue repeat."
      Kuehnel K. , Jarchau T. , Wolf E. , Schlichting I. , Walter U. , Wittinghofer A. , Strelkov S.V.
      Proc. Natl. Acad. Sci. U.S.A.101:17027-17032(2004) [PubMed] [Europe PMC] [Abstract]
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      "Structural basis for the recruitment of profilin-actin complexes during filament elongation by Ena/VASP."
      Ferron F. , Rebowski G. , Lee S.H. , Dominguez R.
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      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 203-245 IN COMPLEXES WITH PFN1 AND ACTIN;INTERACTION WITH PFN1 AND MONOMERIC ACTIN
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      "Modulation of actin structure and function by phosphorylation of Tyr-53 and profilin binding."
      Baek K. , Liu X. , Ferron F. , Shu S. , Korn E.D. , Dominguez R.
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      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 199-214 IN COMPLEX WITH PFN1 AND ACTIN
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