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Index > Protein center > ADIPOQ(Gene name) > Bovine
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  • ADIPOQ (Gene name),
  • Adiponectin (Protein name ),  ADIPO_BOVIN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Gene name:
    ADIPOQ(ACRP30;APM1);
    Protein name:
    Adiponectin;
    Alternative:
    Adipocyte complement-related 30 kDa protein(ACRP30);30 kDa adipocyte complement-related protein;Adipose most abundant gene transcript 1 protein(apM-1);Adipocyte, C1q and collagen domain-containing protein;
    Organism:
    Bovine (Bos taurus). 
    General Annotation
    Sub Unit:
    Homomultimer. Forms trimers, hexamers and 12- to 18-mers. The trimers (low molecular weight complexes / LMW) are assembled via non-covalent interactions of the collagen-like domains in a triple helix and hydrophobic interactions within the globular C1q domain. Several trimers can associate to form disulfide-linked hexamers (middle molecular weight complexes / MMW) and larger complexes (higher molecular weight / HMW). The HMW-complex assembly may rely aditionally on lysine hydroxylation and glycosylation. LMW, MMW and HMW complexes bind to HBEGF, MMW and HMW complexes bind to PDGFB, and HMW complex binds to FGF2. Interacts with CTRP9 via the C1q domain (heterotrimeric complex).
    Function:
    Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
    Subcellular Location:
    Secreted
    Protein Attributes:
    Sequence length:
    240
    Sequence:
    50:
    MLLQGALLLL | LALPSHGEDN | MEDPPLPKGA | CAGWMAGIPG | HPGHNGTPGR | 
    100:
    DGRDGTPGEK | GEKGDPGLVG | PKGDTGETGI | TGIEGPRGFP | GTPGRKGEPG | 
    150:
    ESAYVYRSAF | SVGLERQVTV | PNVPIRFTKI | FYNQQNHYDG | TTGKFLCNIP | 
    200:
    GLYYFSYHIT | VYLKDVKVSL | YKNDKALLFT | HDQFQDKNVD | QASGSVLLYL | 
    240:
    EKGDQVWLQV | YEGENHNGVY | ADNVNDSTFT | GFLLYHNIVE | 
    3D Structure:
    N/A
    Predicted Eptitope:
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    EIAab Sequence  Vaild Sequence:
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    Related Databases
    UniGene:
    String:
    Pfam:
    UniGene:
    SMR:
    Uniprot:
     
    FOR
    ELISA Kit for Bovine Adiponectin
    Cat.:
    E0605h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Bovine Adiponectin
    Cat.:
    E0605m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Bovine Adiponectin
    Cat.:
    E0605d
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Bovine Adiponectin
    Cat.:
    E0605b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Bovine Adiponectin
    Cat.:
    E0605r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Bovine Adiponectin
    Cat.:
    U0605b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Bovine Adiponectin
    Cat.:
    U0605d
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Bovine Adiponectin
    Cat.:
    U0605r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Bovine Adiponectin
    Cat.:
    U0605h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Bovine Adiponectin
    Cat.:
    U0605m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Bovine Adiponectin
    Cat.:
    P0605Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Bovine Adiponectin
    Polyclonal Antibody for Bovine Adiponectin
    Cat.:
    P0605Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Bovine Adiponectin
    Polyclonal Antibody for Bovine Adiponectin
    Cat.:
    P0605Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Bovine Adiponectin
    Monoclonal Antibody for Bovine Adiponectin
    Monoclonal Antibody for Bovine Adiponectin
    Monoclonal Antibody for Bovine Adiponectin
    Monoclonal Antibody for Bovine Adiponectin
    Protein for Bovine Adiponectin
    Protein for Bovine Adiponectin
    Protein for Bovine Adiponectin
    Protein for Bovine Adiponectin
    Protein for Bovine Adiponectin
    Cat.:
    R0605h
    Price:
    Please sign in first.
    Packing:
    1mg
    Purity:
    >90%

    R&D Technical Data
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               12% SDS-PAGE Analysis

     

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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤ 4.5%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤ 7.1%

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    Recovery
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    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    95

    91-98

    Plasma

    97

    92-102

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    87-95%

    90-101%

    92-103%

    90-98%

    EDTA plasma(n=5)

    89-95%

    91-102%

    89-101%

    90-102%

    heparin plasma(n=5)

    90-98%

     

    92-105%

    96-101%

    89-99%

     

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    References
    1. 1.
      "Identification and adipocyte differentiation-dependent expression of the unique disialic acid residue in an adipose tissue-specific glycoprotein, adipo Q."
      Sato C. , Yasukawa Z. , Honda N. , Matsuda T. , Kitajima K.
      J. Biol. Chem.276:28849-28856(2001) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];PROTEIN SEQUENCE OF 18-26; 194-197; 203-208 AND 221-228;GLYCOSYLATION;STRUCTURE OF CARBOHYDRATES
      tissue: Adipose tissue.
    2. 2.
      Morsci N.S. , Schnabel R.D. , Taylor J.F.
      Submitted (2005-08) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    3. 3.
      NIH - Mammalian Gene Collection (MGC) project
      Submitted (2007-04) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      strain: Hereford.
      tissue: Ascending colon.
    4. 4.
      "Proteomic and functional characterization of endogenous adiponectin purified from fetal bovine serum."
      Wang Y. , Lu G. , Wong W.P.S. , Vliegenthart J.F.G. , Gerwig G.J. , Lam K.S.L. , Cooper G.J.S. , Xu A.
      Proteomics4:3933-3942(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 18-26;PARTIAL PROTEIN SEQUENCE;HYDROXYLATION AT LYS-28; PRO-39; PRO-42; PRO-48; LYS-60; LYS-63; LYS-72; PRO-86 AND LYS-96;GLYCOSYLATION AT LYS-28; LYS-60; LYS-63 AND LYS-96;ABSENCE OF HYDROXYLATION AT PRO-57; PRO-66; PRO-71; PRO-90 AND PRO-99;ABSENCE OF GLYCOSYLATION AT ASN-225;IDENTIFICATION BY MASS SPECTROMETRY
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