FEN1 ELISA Kit for Human(Homo sapiens) | EIAab.comFEN1 ELISA Kit for Human(Homo sapiens)

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Index > Protein center > FEN1(Gene name) > Human

FEN1 (Gene name),

Flap endonuclease 1 (Protein name ), FEN1_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

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Gene name:

FEN1(RAD2);

Protein name:

Flap endonuclease 1(FEN-1);

Alternative:

Flap structure-specific endonuclease 1;DNase IV;Maturation factor 1(MF1;hFEN-1);

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Interacts with PCNA. Three molecules of FEN1 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity. The C-terminal domain binds EP300. Can bind simultaneously to both PCNA and EP300. Interacts with DDX11.

Function:

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.

Subcellular Location:

Nucleus nucleolus Nucleus nucleoplasm Mitochondrion Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.

Protein Attributes:

Sequence length:

380

Sequence:

50:

100:

150:

200:

250:

300:

350:

380:

SSAKRKEPEP | KGSTKKKAKT | GAAGKFKRGK |

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

MIM:

600393

Pfam:

PF00867

STRING:

9606.ENSP00000305480

UniGene:

Hs.409065

String:

P39748

SMR:

P39748

KEGG:

hsa:2237

Uniprot:

P39748

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ELISA Kit for Human FEN-1

Cat.:

E15243h

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96T

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ELISA Kit for Human FEN-1

Cat.:

E15243m

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96T

ELISA Kit for Human FEN-1

Cat.:

E15243r

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96T

ELISA Kit for Human FEN-1

Cat.:

E15243c

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96T

ELISA Kit for Human FEN-1

Cat.:

E15243b

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96T

CLIA Kit for Human FEN-1

Cat.:

U15243r

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96T

CLIA Kit for Human FEN-1

Cat.:

U15243c

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96T

CLIA Kit for Human FEN-1

Cat.:

U15243h

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96T

CLIA Kit for Human FEN-1

Cat.:

U15243b

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96T

CLIA Kit for Human FEN-1

Cat.:

U15243m

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96T

Polyclonal Antibody for Human FEN-1

Cat.:

P15243Rb-m

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40ug/0.2ml

Polyclonal Antibody for Human FEN-1

Cat.:

P15243Rb-h

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40ug/0.2ml

Polyclonal Antibody for Human FEN-1

Cat.:

P15243Rb-r

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40ug/0.2ml

Monoclonal Antibody for Human FEN-1

Protein for Human FEN-1

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

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Linearity

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References

1.

"Structural and functional conservation of the human homolog of the Schizosaccharomyces pombe rad2 gene, which is required for chromosome segregation and recovery from DNA damage."
Murray J.M. , Tavassoli M. , Al-Harithy R. , Sheldrick K.S. , Lehmann A.R. , Carr A.M. , Watts F.Z.
Mol. Cell. Biol.14:4878-4888(1994) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
2.

"Sequence of human FEN-1, a structure-specific endonuclease, and chromosomal localization of the gene (FEN1) in mouse and human."
Hiraoka L.R. , Harrington J.J. , Gerhard D.S. , Lieber M.R. , Hsieh C.-L.
Genomics25:220-225(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
tissue: Leukemic T-cell.
3.

NIEHS SNPs program
Submitted (2002-06) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
4.

"Human chromosome 11 DNA sequence and analysis including novel gene identification."
Taylor T.D. , Noguchi H. , Totoki Y. , Toyoda A. , Kuroki Y. , Dewar K. , Lloyd C. , Itoh T. , Takeda T. , Kim D.-W. , She X. , Barlow K.F. , Bloom T. , Bruford E. , Chang J.L. , Cuomo C.A. , Eichler E. , FitzGerald M.G. , more...
Nature440:497-500(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
5.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
tissue: Lung.
6.

"Structural and functional homology between mammalian DNase IV and the 5'-nuclease domain of Escherichia coli DNA polymerase I."
Robins P. , Pappin D.J.C. , Wood R.D. , Lindahl T.
J. Biol. Chem.269:28535-28538(1994) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PARTIAL PROTEIN SEQUENCE;FUNCTION;SUBCELLULAR LOCATION
7.

"Essential amino acids for substrate binding and catalysis of human flap endonuclease 1."
Shen B. , Nolan J.P. , Sklar L.A. , Park M.S.
J. Biol. Chem.271:9173-9176(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;CATALYTIC ACTIVITY;SUBSTRATE-BINDING SITES;MUTAGENESIS OF ASP-34; ASP-86; ARG-103; GLU-158; ASP-179; ASP-181; GLY-231 AND ASP-233
8.

"The DNA repair endonuclease XPG binds to proliferating cell nuclear antigen (PCNA) and shares sequence elements with the PCNA-binding regions of FEN-1 and cyclin-dependent kinase inhibitor p21."
Gary R. , Ludwig D.L. , Cornelius H.L. , MacInnes M.A. , Park M.S.
J. Biol. Chem.272:24522-24529(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH PCNA
9.

"Mechanism whereby proliferating cell nuclear antigen stimulates flap endonuclease 1."
Tom S. , Henricksen L.A. , Bambara R.A.
J. Biol. Chem.275:10498-10505(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
10.

"Regulation of human flap endonuclease-1 activity by acetylation through the transcriptional coactivator p300."
Hasan S. , Stucki M. , Hassa P.O. , Imhof R. , Gehrig P. , Hunziker P. , Hubscher U. , Hottiger M.O.
Mol. Cell7:1221-1231(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH P300;ACETYLATION AT LYS-354; LYS-375; LYS-377 AND LYS-380
11.

"Arginine residues 47 and 70 of human flap endonuclease-1 are involved in DNA substrate interactions and cleavage site determination."
Qiu J. , Bimston D.N. , Partikian A. , Shen B.
J. Biol. Chem.277:24659-24666(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;SUBSTRATE-BINDING SITES ARG-47 AND ARG-70;MUTAGENESIS OF ARG-29; ARG-47; ARG-70; ARG-73 AND LYS-80
12.

"Studies with the human cohesin establishment factor, ChlR1. Association of ChlR1 with Ctf18-RFC and Fen1."
Farina A. , Shin J.H. , Kim D.H. , Bermudez V.P. , Kelman Z. , Seo Y.S. , Hurwitz J.
J. Biol. Chem.283:20925-20936(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH DDX11
13.

"Nucleolar localization and dynamic roles of flap endonuclease 1 in ribosomal DNA replication and damage repair."
Guo Z. , Qian L. , Liu R. , Dai H. , Zhou M. , Zheng L. , Shen B.
Mol. Cell. Biol.28:4310-4319(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;SUBCELLULAR LOCATION;PHOSPHORYLATION;MUTAGENESIS OF SER-187
14.

"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Choudhary C. , Kumar C. , Gnad F. , Nielsen M.L. , Rehman M. , Walther T.C. , Olsen J.V. , Mann M.
Science325:834-840(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-80 AND LYS-375;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
15.

"Methylation of FEN1 suppresses nearby phosphorylation and facilitates PCNA binding."
Guo Z. , Zheng L. , Xu H. , Dai H. , Zhou M. , Pascua M.R. , Chen Q.M. , Shen B.
Nat. Chem. Biol.6:766-773(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;MUTAGENESIS OF ARG-192;METHYLATION AT ARG-19; ARG-100; ARG-104 AND ARG-192;PHOSPHORYLATION AT SER-187
16.

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V. , Vermeulen M. , Santamaria A. , Kumar C. , Miller M.L. , Jensen L.J. , Gnad F. , Cox J. , Jensen T.S. , Nigg E.A. , Brunak S. , Mann M.
Sci. Signal.3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-197 AND THR-364;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
17.

"Initial characterization of the human central proteome."
Burkard T.R. , Planyavsky M. , Kaupe I. , Breitwieser F.P. , Buerckstuemmer T. , Bennett K.L. , Superti-Furga G. , Colinge J.
BMC Syst. Biol.5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
18.

"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P. , Lasa M. , Polevoda B. , Gazquez C. , Elosegui-Artola A. , Kim D.S. , De Juan-Pardo E. , Demeyer K. , Hole K. , Larrea E. , Timmerman E. , Prieto J. , Arnesen T. , Sherman F. , Gevaert K. , Aldabe R.
Proc. Natl. Acad. Sci. U.S.A.109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
19.

"A cryptic targeting signal creates a mitochondrial FEN1 isoform with tailed R-loop binding properties."
Kazak L. , Reyes A. , He J. , Wood S.R. , Brea-Calvo G. , Holen T.T. , Holt I.J.
PLoS ONE8:E62340-E62340(2013) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ALTERNATIVE INITIATION (ISOFORM FENMIT);SUBCELLULAR LOCATION (ISOFORM FENMIT)
20.

"Structural and thermodynamic analysis of human PCNA with peptides derived from DNA polymerase-delta p66 subunit and flap endonuclease-1."
Bruning J.B. , Shamoo Y.
Structure12:2209-2219(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.88 ANGSTROMS) OF 331-350
21.

"Structural basis for recruitment of human flap endonuclease 1 to PCNA."
Sakurai S. , Kitano K. , Yamaguchi H. , Hamada K. , Okada K. , Fukuda K. , Uchida M. , Ohtsuka E. , Morioka H. , Hakoshima T.
EMBO J.24:683-693(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) OF 2-380 IN COMPLEX WITH PCNA

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