OXT ELISA Kit for Bovine(Bos taurus) | EIAab.comOXT ELISA Kit for Bovine(Bos taurus) | EIAab.com

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OXT (Gene name),

Oxytocin-neurophysin 1 (Protein name ), NEU1_BOVIN from NCBI database.

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General Annotation

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Antigen Annotation

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3D

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Predicted Eptitope

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Vaild Sequence

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Related Databases

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Gene name:

OXT;

Protein name:

Oxytocin-neurophysin 1(OT-NPI);

Alternative:

Organism:

OXT (Bos taurus).

General Annotation

Sub Unit:

Function:

Oxytocin causes contraction of the smooth muscle of the uterus and of the mammary gland.

Subcellular Location:

Secreted

Protein Attributes:

Sequence length:

125

Sequence:

50:

MAGSSLACCL | LGLLALTSAC | YIQNCPLGGK | RAVLDLDVRT | CLPCGPGGKG |

100:

RCFGPSICCG | DELGCFVGTA | EALRCQEENY | LPSPCQSGQK | PCGSGGRCAA |

125:

AGICCSPDGC | HEDPACDPEA | AFSQH

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

UniGene:

String:

SMR:

Pfam:

KEGG:

STRING:

9913.ENSBTAP00000010554

Uniprot:

FOR

ELISA Kit for Bovine OT-NPI

Cat.:

E15341m

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96T

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ELISA Kit for Bovine OT-NPI

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E15341h

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96T

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ELISA Kit for Bovine OT-NPI

Cat.:

E15341r

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96T

ELISA Kit for Bovine OT-NPI

Cat.:

E15341b

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ELISA Kit for Bovine OT-NPI

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ELISA Kit for Bovine OT-NPI

Cat.:

E15341p

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96T

CLIA Kit for Bovine OT-NPI

Cat.:

U15341p

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96T

CLIA Kit for Bovine OT-NPI

MSDS:

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CLIA Kit for Bovine OT-NPI

Cat.:

U15341r

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96T

CLIA Kit for Bovine OT-NPI

Cat.:

U15341b

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Packing:

96T

CLIA Kit for Bovine OT-NPI

Cat.:

U15341m

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MSDS:

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Packing:

96T

CLIA Kit for Bovine OT-NPI

Cat.:

U15341h

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96T

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Polyclonal Antibody for Bovine OT-NPI

Cat.:

P15341Rb-m

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40ug/0.2ml

Polyclonal Antibody for Bovine OT-NPI

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P15341Rb-h

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Polyclonal Antibody for Bovine OT-NPI

Polyclonal Antibody for Bovine OT-NPI

Polyclonal Antibody for Bovine OT-NPI

Cat.:

P15341Rb-r

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Packing:

40ug/0.2ml

Polyclonal Antibody for Bovine OT-NPI

Monoclonal Antibody for Bovine OT-NPI

Monoclonal Antibody for Bovine OT-NPI

Monoclonal Antibody for Bovine OT-NPI

Monoclonal Antibody for Bovine OT-NPI

Monoclonal Antibody for Bovine OT-NPI

Monoclonal Antibody for Bovine OT-NPI

Protein for Bovine OT-NPI

Protein for Bovine OT-NPI

Protein for Bovine OT-NPI

Protein for Bovine OT-NPI

Protein for Bovine OT-NPI

Protein for Bovine OT-NPI

R&D Technical Data

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s

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s

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Precision

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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

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Recovery

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Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

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Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

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Linearity

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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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References

1.

"Deduced amino acid sequence from the bovine oxytocin-neurophysin I precursor cDNA."
Land H. , Grez M. , Ruppert S. , Schmale H. , Rehbein M. , Richter D. , Schuetz G.
Nature302:342-344(1983) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
2.

"Recent gene conversion involving bovine vasopressin and oxytocin precursor genes suggested by nucleotide sequence."
Ruppert S. , Scherer G. , Schuetz G.
Nature308:554-557(1984) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
3.

"The neurohypophyseal hormones vasopressin and oxytocin. Precursor structure, synthesis and regulation."
Rehbein M. , Hillers M. , Mohr E. , Ivell R. , Morley S. , Schmale H. , Richter D.
Biol. Chem. Hoppe-Seyler367:695-704(1986) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
4.

NIH - Mammalian Gene Collection (MGC) project
Submitted (2007-06) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
strain: Hereford.
tissue: Hypothalamus.
5.

"The gene for the hypothalamic peptide hormone oxytocin is highly expressed in the bovine corpus luteum: biosynthesis, structure and sequence analysis."
Ivell R. , Richter D.
EMBO J.3:2351-2354(1984) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 15-125
6.

"The sequence of amino acids in oxytocin, with a proposal for the structure of oxytocin."
du Vigneaud V. , Ressler C. , Trippett S.
J. Biol. Chem.205:949-957(1953) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 20-28;DISULFIDE BONDS;AMIDATION AT GLY-28
7.

"About the chemical structure of oxytocin."
Tuppy H. , Michl H.
Monatsh. Chem.84:1011-1020(1953)

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 20-28
8.

"Complete amino acid sequence of bovine neurophysin-I. A major secretory product of the posterior pituitary."
Schlesinger D.H. , Audhya T.K. , Walter R.
J. Biol. Chem.253:5019-5024(1978) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 32-124
9.

"Comparison between MSEL- and VLDV-neurophysins. Complete amino acid sequences of porcine and bovine VLDV-neurophysins."
Chauvet M.-T. , Codogno P. , Chauvet J. , Acher R.
FEBS Lett.98:37-40(1979) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 32-124
10.

"Complete assignment of neurophysin disulfides indicates pairing in two separate domains."
Burman S. , Wellner D. , Chait B. , Chaudhary T. , Breslow E.
Proc. Natl. Acad. Sci. U.S.A.86:429-433(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DISULFIDE BONDS
11.

"Crystal structure analysis of deamino-oxytocin: conformational flexibility and receptor binding."
Wood S.P. , Tickle I.J. , Treharne A.M. , Pitts J.E. , Mascarenhas Y. , Li J.Y. , Husain J. , Cooper S. , Blundell T.L. , Hruby V.J.
Science232:633-636(1986) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.04 ANGSTROMS) OF OXYTOCIN;DISULFIDE BOND;AMIDATION AT GLY-28

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