[Sign in] [Register]   

EIAab logo

EIAab news detail, please contact if you have any questions about online orders and payment.
Enantioselective thyroid disruption in zebrafish embryo-larvae via exposure to environmental concentrations of the chloroacetamide herbicide acetochlor
Update time:2018-11-29 11:05:00   【 Font: Large  Medium Small


Acetochlor (ACT) is a chiral chloroacetamide pesticide that has been heavily used around the world, resulting in its residues being frequently found in surface waters. It has been reported that ACT is an endocrine disrupting chemical (EDC) with strong thyroid hormone-disrupting activity in aquatic organisms. However, the enantioselectivity underlying thyroid disruption has yet to be understood. In this study, using a zebrafish embryo-larvae model, the enantioselective thyroid disruption of ACT was investigated at a series of environmentally relevant concentrations (1, 2, 10 and 50?μg/L). Our results showed that both racemic ACT and its enantiomers significantly increased the malformation rates of embryos at 72?h postfertilization (hpf). Decreased thyroxine (T4) contents and increased triiodothyronine (T3) contents were found in larvae at 120?hpf, with (+)-S-ACT exhibiting a greater effect than (?)-R-enantiomer. Similarly, (+)-S-ACT also showed a stronger effect on the mRNA expressions of thyroid hormone receptors (TRα and TRβ), deiodinase2 (Dio2) and thyroid-stimulating hormone-β (TSHβ) genes. The observed enantioselectivity in TR expressions was consistent with that of in silico binding analysis, which suggested that (+)-S-enantiomer binds more potently to the TRs than (?)-R-enantiomer. In general, ACT enantiomers showed different influences on the secretion of THs, expression of TH-related key genes and binding affinity to TRs. Considering the different toxicity of different enantiomers, our study highlights the importance of enantioselectivity in understanding of thyroid disruption effects of chiral pesticides.

Cited products
Source:Science of The Total Environment      by C Xu, X Sun, L Niu, et al.
Hot Genes
Atf2 ASPRO ACE ALCAM C19orf80 Trap1a KSR2 Gdf5
Top Searches
Ubiquitin-protein ligase metalloproteinase Ubiquitin ELISA Tumor necrosis Alpha Asprosin TRAP1A
Why choose EIAAB
Our products have been quoted by many publications in famous journals such as Cell; Cell Metabolism; Hepatology; Biomaterials.more
Further Information
About us Protein center Bank account Distributors Terms & Conditions Career

Copyright & copy www.eiaab.com2006-2016 All Rights Reserved    EIAab


鄂公网安备 42018502005535号