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Index > Protein center > VB12(Shorten name) > General
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  • General Annotation
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  • General Annotation
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Shorten name:
    VB12;
    Antigen name:
    Vitamin B12;
    Alternative:
    cobalamin;
    General Annotation
    Sub Unit:
    Function:
    It is normally involved in the metabolism of every cell of the human body, especially affecting DNA synthesis and regulation, but also fatty acid synthesis and energy production.
    Location:
    Vitamin B12, vitamin B12 or vitamin B-12, also called cobalamin, is a water-soluble vitamin with a key role in the normal functioning of the brain and nervous system, and for the formation of blood. It is one of the eight B vitamins.
    Antigen Attributes:
    Formula:
    C63H88CoN14O14P
    3D Structure:
    N/A
    Predicted Eptitope:
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    EIAab Sequence  Vaild Sequence:
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    FOR
    ELISA Kit for General Vitamin B12
    Cat.:
    E0924Ge
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for General Vitamin B12
    Cat.:
    U0924Ge
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.

    R&D Technical Data

    s

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Precision

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤4.3%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.6%

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Recovery

    Recovery was determined by spiking various levels of VB12 into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    95-103

    Plasma

    98

    97-102

     

     

     

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Linearity

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VB12 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    97-102%

    96-101%

    92-105%

    94-106%

    EDTA plasma(n=5)

    89-95%

    93-105%

    98-106%

    97-101%

    heparin plasma(n=5)

    93-99%

     

    97-100%

    102-108%

    94-106%

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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