ASPRO ELISA Kit for Human() | EIAab.comASPRO ELISA Kit for Human()

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ASPRO (Shorten name),

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General Annotation

Predicted Eptitope

Vaild Sequence

Citations (5)

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Shorten name:

ASPRO;

Antigen name:

Asprosin;

Alternative:

General Annotation

Sub Unit:

Asprosin, a fasting-induced glucogenic protein homone that modulates hepatic glucose release. It is the C-terminal cleavage product of profibrillin, and encoded by the ultimate two exons of FBN1.

Function:

Circulating asprosin, a protein hormone, responds to low dietary glucose by triggering the release of liver glucose stores, and the reduction of asprosin protects against the hyperinsulinism associated with metabolic syndrome.

Location:

Asprosin circulates at nanomolar levels, a single dose of recombinant asprosin retains biological activity and elevates blood glucose and insulin levels. Liver is the target organ for asprosin to recruit. Asprosin increases hepatocyte glucose release through activating the G protein-cAMP-PKA pathway.

Antigen Attributes:

Formula:

STNETDASNIEDQSETEANVSLASWDVEKTAIFAFNISHVSNKVRILELLPALTTLTNHN RYLIESGNEDGFFKINQKEGISYLHFTKKKPVAGTYSLQISSTPLYKKKELNQLEDKYDK DYLSGELGDNLKMKIQVLLH

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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ELISA Kit for Human Asprosin

Cat.:

E15190h

Price:

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Manual:

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MSDS:

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Packing:

96T

Range:

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CLIA Kit for Human Asprosin

Cat.:

U15190h

Price:

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MSDS:

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Packing:

96T

Polyclonal Antibody for Human Asprosin

Cat.:

P15190Rb-h

Price:

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Packing:

40ug/0.2ml

Monoclonal Antibody for Human Asprosin

Protein for Human Asprosin

R&D Technical Data

IPTG induced Asprosin protein expression

12% SDS-PAGE analysis

Western blot - Anti- Asprosin antibody

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤ 6.5%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤ 9.8%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	93-105
Plasma	101	95-107

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	97-105%	93-101%	95-104%	99-109%
EDTA plasma(n=5)	90-99%	98- 105%	89-97%	95-106%
heparin plasma(n=5)	95-104%	99-110%	100-111%	97-110%