Gene name:
Aak1 (Kiaa1048) ;
Protein name:
AP2-associated protein kinase 1 ;
Alternative:
Adaptor-associated kinase 1 ;
Organism:
Mouse (Mus musculus).
General Annotation
Sub Unit:
Interacts with alpha-adaptin, AP-2, clathrin, NUMB and EPS15 isoform 2 (By similarity). Interacts with membrane-bound activated NOTCH1 but not with the inactive full-length form of NOTCH1. Preferentially interacts with monoubiquitinated activated NOTCH1 compared to the non-ubiquitinated form.
Function:
Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity.
Subcellular Location:
Cell membrane
Peripheral membrane protein
Membrane
clathrin-coated pit
Active when found in clathrin-coated pits at the plasma membrane. In neuronal cells, enriched at presynaptic terminals. In non-neuronal cells, enriched at leading edge of migrating cells.
Protein Attributes:
50:
MKKFFDSRRE | QGSSGLGSGS | SGGGGSSSGL | GSGYIGRVFG | IGRQQVTVDE |
100:
VLAEGGFALV | FLVRTSNGVK | CALKRMFVNN | EHDLQVCKRE | IQIMRDLSGH |
150:
KNIVGYIDSS | INNVSSGDVW | EVLILMDFCR | GGQVVNLMNQ | RLQTGFTENE |
200:
VLQIFCDTCE | AVARLHQCKT | PIIHRDLKVE | NILLHDRGHY | VLCDFGSATN |
250:
KFQNPQAEGV | NAVEDEIKKY | TTLSYRAPEM | VNLYSGKIIT | TKADIWALGC |
300:
LLYKLCYFTL | PFGESQVAIC | DGSFTIPDNS | RYSQDMHCLI | RYMLEPDPDK |
350:
RPDIYQVSYF | SFKLLKKECP | VPNVQNSPIP | AKLPEPVKAS | EAAVKKTQPK |
400:
ARLTDPIPTT | ETSIAPRQRP | KAGQTQPNPG | ILPIQPALTP | RKRATVQPLP |
450:
QAAGPSNQPG | LLPSVSQPKA | QATPSQPLQS | SQPKQPQAPP | TPQQTPATQT |
500:
QGLPTQAQAT | PQHQQQHLLK | QQQQQQQQPQ | QPTAPPQPAG | TFYQQQQQQQ |
550:
QQQAQTQQFQ | AVHPAAQQPV | TAQFPVGSQG | GAQQQLMQNF | YHQQQQQQQQ |
600:
QQQLMAQQAA | LQQKTAVVVP | QSQAQPATAP | QAAAAQEPGQ | IQAPVRQQPK |
650:
VQTTPPPTIQ | GQKVGSLTPP | SSPKTQRAGH | RRILSDVTHS | AVFGVPASKS |
700:
TQLLQAAAAE | ASLNKSKSAT | TTPSGSPRTS | QQNVSNASEG | STWNPFDDDN |
750:
FSKLTAEELL | NKDFAKLGEG | KLPEKLGGSA | ESLIPGFQPT | QGDAFTTPSF |
800:
SAGTAEKRKG | GQAVDSGIPL | LSVSDPFIPL | QVPDAPEKLI | EGLKSPDTSL |
850:
LLPDLLPMTD | PFGSTSDAVI | DKADVAVESL | IPGLEPPVAQ | RLPSQTESVT |
900:
SNRTDSLTGE | DSLLDCSLLS | NPTAGLLEEF | APIALSAPTH | KAAEDSNLIS |
950:
GFGVAEGSEK | VAEDEFDPIP | VLITKNTQGG | HSRNSSGSSE | SSLPNLARSL |
Vaild Sequence:
Related Databases
Uniprot:
ELISA Kit
CLIA Kit
Polyclonal Antibody
Monoclonal Antibody
Protein
FOR
Rat
Pig
Bovine
Mouse
Human
ELISA Kit for Mouse AP2-associated protein kinase 1
ELISA Kit for Mouse AP2-associated protein kinase 1
ELISA Kit for Mouse AP2-associated protein kinase 1
ELISA Kit for Mouse AP2-associated protein kinase 1
ELISA Kit for Mouse AP2-associated protein kinase 1
CLIA Kit for Mouse AP2-associated protein kinase 1
CLIA Kit for Mouse AP2-associated protein kinase 1
CLIA Kit for Mouse AP2-associated protein kinase 1
CLIA Kit for Mouse AP2-associated protein kinase 1
CLIA Kit for Mouse AP2-associated protein kinase 1
Polyclonal Antibody for Mouse AP2-associated protein kinase 1
Polyclonal Antibody for Mouse AP2-associated protein kinase 1
Polyclonal Antibody for Mouse AP2-associated protein kinase 1
Polyclonal Antibody for Mouse AP2-associated protein kinase 1
Polyclonal Antibody for Mouse AP2-associated protein kinase 1
Monoclonal Antibody for Mouse AP2-associated protein kinase 1
Monoclonal Antibody for Mouse AP2-associated protein kinase 1
Monoclonal Antibody for Mouse AP2-associated protein kinase 1
Monoclonal Antibody for Mouse AP2-associated protein kinase 1
Monoclonal Antibody for Mouse AP2-associated protein kinase 1
Protein for Mouse AP2-associated protein kinase 1
Protein for Mouse AP2-associated protein kinase 1
Protein for Mouse AP2-associated protein kinase 1
Protein for Mouse AP2-associated protein kinase 1
Protein for Mouse AP2-associated protein kinase 1
R&D Technical Data
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Precision
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Recovery
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Linearity
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References
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more...
Science309:1559-1563(2005)
[
PubMed ]
[
Europe PMC ]
[
Abstract ]
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
strain :
C57BL/6J .
tissue :
Visual cortex .
2.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1);NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 473-959 (ISOFORM 2)
strain :
C57BL/6 .
tissue :
Brain .
3.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 417-959 (ISOFORM 1)
tissue :
Brain .
4.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue :
Brain .
5.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-635;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue :
Liver .
6.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-635;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
7.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-604; THR-618 AND SER-622;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue :
Embryonic fibroblast .
8.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : INTERACTION WITH NOTCH1