Angiotensin-converting enzyme (Protein name
), ACE_BOVIN from NCBI database.
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General Annotation
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Antigen Annotation
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3D
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Predicted Eptitope
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Vaild Sequence
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Gene name:
ACE(DCP1);
Protein name:
Angiotensin-converting enzyme(ACE);
Alternative:
Kininase II;Dipeptidyl carboxypeptidase I;
CD:
CD143;
Organism:
Bovine (Bos taurus).
General Annotation
Sub Unit:
Function:
Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety.
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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤3.9%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.7%
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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.0%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.9%
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Recovery
Recovery was determined by spiking various levels of ACE into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
99
91-103
Plasma
98
93-106
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Recovery was determined by spiking various levels of ACE into serum and plasma .
Sample Type
Average(%)
Recovery Range (%)
Serum
101
95-107
Plasma
99
93-102
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Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1：2
1:4
1:8
1:16
serum(n=5)
92-105%
90-101%
95-105%
92-109%
Saliva(n=3)
89-95%
93105%
89-94%
90-106%
heparin plasma(n=5)
93-104%
94-110%
101-111%
91-110%
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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1：2
1:4
1:8
1:16
serum(n=5)
93-104%
91-101%
95-102%
96-109%
Saliva(n=3)
90-99%
93- 99%
89-97%
92-106%
heparin plasma(n=5)
94-103%
96-110%
99-111%
94-110%
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References
1.
"Analysis of the angiotensin converting enzyme promoter from Bos taurus." Mungunsukh O.
,
Day R.M.
Submitted (2007-11) to the EMBL/GenBank/DDBJ databases
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Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
Vaild Sequence:
