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Index > Protein center > ADRA2A(Gene name) > Human
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  • ADRA2A (Gene name),
  • Alpha-2A adrenergic receptor (Protein name ),  ADA2A_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Gene name:
    ADRA2A(ADRA2R;ADRAR);
    Protein name:
    Alpha-2A adrenergic receptor;
    Alternative:
    Alpha-2A adrenoreceptor(Alpha-2A adrenoceptor;Alpha-2AAR);Alpha-2 adrenergic receptor subtype C10;
    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    N/A
    Function:
    Alpha-2 adrenergic receptors mediate the catecholamine-induced inhibition of adenylate cyclase through the action of G proteins. The rank order of potency for agonists of this receptor is oxymetazoline > clonidine > epinephrine > norepinephrine > phenylephrine > dopamine > p-synephrine > p-tyramine > serotonin = p-octopamine. For antagonists, the rank order is yohimbine > phentolamine = mianserine > chlorpromazine = spiperone = prazosin > propanolol > alprenolol = pindolol.
    Subcellular Location:
    Cell membrane Multi-pass membrane protein
    Protein Attributes:
    Sequence length:
    450
    Sequence:
    50:
    MGSLQPDAGN | ASWNGTEAPG | GGARATPYSL | QVTLTLVCLA | GLLMLLTVFG | 
    100:
    NVLVIIAVFT | SRALKAPQNL | FLVSLASADI | LVATLVIPFS | LANEVMGYWY | 
    150:
    FGKAWCEIYL | ALDVLFCTSS | IVHLCAISLD | RYWSITQAIE | YNLKRTPRRI | 
    200:
    KAIIITVWVI | SAVISFPPLI | SIEKKGGGGG | PQPAEPRCEI | NDQKWYVISS | 
    250:
    CIGSFFAPCL | IMILVYVRIY | QIAKRRTRVP | PSRRGPDAVA | APPGGTERRP | 
    300:
    NGLGPERSAG | PGGAEAEPLP | TQLNGAPGEP | APAGPRDTDA | LDLEESSSSD | 
    350:
    HAERPPGPRR | PERGPRGKGK | ARASQVKPGD | SLPRRGPGAT | GIGTPAAGPG | 
    400:
    EERVGAAKAS | RWRGRQNREK | RFTFVLAVVI | GVFVVCWFPF | FFTYTLTAVG | 
    450:
    3D Structure:
    N/A
    Predicted Eptitope:
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    EIAab Sequence  Vaild Sequence:
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    Related Databases
    String:
    MIM:
    UniGene:
    Pfam:
    KEGG:
    SMR:
    Uniprot:
     
    FOR
    ELISA Kit for Human Alpha-2A adrenergic receptor
    Cat.:
    E14916h
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    MSDS:
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    96T
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    ELISA Kit for Human Alpha-2A adrenergic receptor
    Cat.:
    E14916r
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    MSDS:
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    Packing:
    96T
    ELISA Kit for Human Alpha-2A adrenergic receptor
    Cat.:
    E14916m
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    96T
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    ELISA Kit for Human Alpha-2A adrenergic receptor
    Cat.:
    E14916b
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    MSDS:
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    Packing:
    96T
    ELISA Kit for Human Alpha-2A adrenergic receptor
    Cat.:
    E14916p
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    MSDS:
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    Packing:
    96T
    CLIA Kit for Human Alpha-2A adrenergic receptor
    Cat.:
    U14916h
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    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Alpha-2A adrenergic receptor
    Cat.:
    U14916p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Alpha-2A adrenergic receptor
    Cat.:
    U14916m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human Alpha-2A adrenergic receptor
    Cat.:
    U14916b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Alpha-2A adrenergic receptor
    Cat.:
    U14916r
    Price:
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    MSDS:
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    Packing:
    96T
    Polyclonal Antibody for Human Alpha-2A adrenergic receptor
    Polyclonal Antibody for Human Alpha-2A adrenergic receptor
    Polyclonal Antibody for Human Alpha-2A adrenergic receptor
    Cat.:
    P14916Rb-h
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Alpha-2A adrenergic receptor
    Cat.:
    P14916Rb-r
    Price:
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Alpha-2A adrenergic receptor
    Cat.:
    P14916Rb-m
    Price:
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    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Human Alpha-2A adrenergic receptor
    Monoclonal Antibody for Human Alpha-2A adrenergic receptor
    Monoclonal Antibody for Human Alpha-2A adrenergic receptor
    Monoclonal Antibody for Human Alpha-2A adrenergic receptor
    Monoclonal Antibody for Human Alpha-2A adrenergic receptor
    Protein for Human Alpha-2A adrenergic receptor
    Protein for Human Alpha-2A adrenergic receptor
    Protein for Human Alpha-2A adrenergic receptor
    Protein for Human Alpha-2A adrenergic receptor
    Protein for Human Alpha-2A adrenergic receptor

    R&D Technical Data
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    s

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    s
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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤5.7%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤8.1%

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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

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    Recovery
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    Recovery was determined by spiking various levels of ADRA2A into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    103

    91-105

    Plasma

    102

    93-106

     

     

     

     

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    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ADRA2A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    100-105%

    96-111%

    95-103%

    98-109%

    EDTA plasma(n=5)

    94-110%

    97-108%

    96-106%

    95-107%

    heparin plasma(n=5)

    93-112%

     

    95-110%

    98-106%

    97-106%

     

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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    EDTA plasma(n=5)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

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    References
    1. 1.
      "Cloning, sequencing, and expression of the gene coding for the human platelet alpha 2-adrenergic receptor."
      Kobilka B.K. , Matsui H. , Kobilka T.S. , Yang-Feng T.L. , Francke U. , Caron M.G. , Lefkowitz R.J. , Regan J.W.
      Science238:650-656(1987) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];PARTIAL PROTEIN SEQUENCE
      tissue: Platelet.
    2. 2.
      "Cloning, sequence analysis, and permanent expression of a human alpha 2-adrenergic receptor in Chinese hamster ovary cells. Evidence for independent pathways of receptor coupling to adenylate cyclase attenuation and activation."
      Fraser C.M. , Arakawa S. , McCombie W.R. , Venter J.C.
      J. Biol. Chem.264:11754-11761(1989) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    3. 3.
      "Cloning, sequencing, and expression of the gene encoding the porcine alpha 2-adrenergic receptor. Allosteric modulation by Na+, H+, and amiloride analogs."
      Guyer C.A. , Horstman D.A. , Wilson A.L. , Clark J.D. , Kragoe E.J. Jr. , Limbird L.E.
      J. Biol. Chem.265:17307-17317(1990) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SEQUENCE REVISION TO 333-365
    4. 4.
      "An Asn to Lys polymorphism in the third intracellular loop of the human alpha 2A-adrenergic receptor imparts enhanced agonist-promoted Gi coupling."
      Small K.M. , Forbes S.L. , Brown K.M. , Liggett S.B.
      J. Biol. Chem.275:38518-38523(2000) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANT LYS-251
    5. 5.
      "Complex haplotypes derived from noncoding polymorphisms of the intronless alpha-2A-adrenergic gene diversify receptor expression."
      Small K.M. , Brown K.M. , Seman C.A. , Theiss C.T. , Liggett S.B.
      Proc. Natl. Acad. Sci. U.S.A.103:5472-5477(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANT LYS-251
    6. 6.
      "Cloning and expression of human alpha-2A adrenergic receptor in SY5Y cells."
      Mao Z.-M. , Tang K. , Li B.-M. , Jing N.-H.
      Submitted (2000-07) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
    7. 7.
      "A search for genetic variability in the human alpha-2 adrenergic receptor on chromosome 10."
      Castellano M. , Giacche' M. , Rossi F. , Rivadossi F. , Perani C. , Beschi M. , Agabiti Rosei E.
      Submitted (2000-08) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    8. 8.
      "Human alpha-2A adrenergic receptor gene and the genotype of -1296 nucleotide and motionsickness."
      Liu L. , Yuan L.
      Submitted (2001-04) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    9. 9.
      SeattleSNPs variation discovery resource
      Submitted (2005-11) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANT LYS-251
    10. 10.
      NHLBI resequencing and genotyping service (RS&G)
      Submitted (2007-12) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    11. 11.
      "The DNA sequence and comparative analysis of human chromosome 10."
      Deloukas P. , Earthrowl M.E. , Grafham D.V. , Rubenfield M. , French L. , Steward C.A. , Sims S.K. , Jones M.C. , Searle S. , Scott C. , Howe K. , Hunt S.E. , Andrews T.D. , Gilbert J.G.R. , Swarbreck D. , Ashurst J.L. , Taylor A. , Battles J. , more...
      Nature429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    12. 12.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      tissue: PNS.
      tissue: Testis.
    13. 13.
      "Identification of an additional gene belonging to the alpha 2 adrenergic receptor family in the human genome by PCR."
      Chhajlani V. , Rangel N. , Uhlen S. , Wikberg J.E.S.
      FEBS Lett.280:241-244(1991) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 77-209
    14. 14.
      "A point mutation in the seventh hydrophobic domain of the alpha 2 adrenergic receptor increases its affinity for a family of beta receptor antagonists."
      Suryanarayana S. , Daunt D.A. , von Zastrow M. , Kobilka B.K.
      J. Biol. Chem.266:15488-15492(1991) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: MUTAGENESIS OF PHE-412
    15. 15.
      "Site-directed mutagenesis of alpha 2a-adrenergic receptors: Identification of amino acids involved in ligand binding and receptor activation by agonists."
      Wang C.-D. , Buck M.A. , Fraser C.M.
      Mol. Pharmacol.40:168-179(1991) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: MUTAGENESIS OF ASPARTIC ACID AND SERINE RESIDUES
    16. 16.
      "Rab26 modulates the cell surface transport of alpha2-adrenergic receptors from the Golgi."
      Li C. , Fan Y. , Lan T.H. , Lambert N.A. , Wu G.
      J. Biol. Chem.287:42784-42794(2012) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;SUBCELLULAR LOCATION
    17. 17.
      "NMR structure of the second intracellular loop of the alpha 2A adrenergic receptor: evidence for a novel cytoplasmic helix."
      Chung D.A. , Zuiderweg E.R.P. , Fowler C.B. , Soyer O.S. , Mosberg H.I. , Neubig R.R.
      Biochemistry41:3596-3604(2002) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 118-149;PROBABLE MEMBRANE TOPOLOGY
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