Pam ELISA Kit for Rat(Rattus norvegicus) | EIAab.comPam ELISA Kit for Rat(Rattus norvegicus)

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Pam (Gene name),

Peptidyl-glycine alpha-amidating monooxygenase (Protein name ), AMD_RAT from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

Pam;

Protein name:

Peptidyl-glycine alpha-amidating monooxygenase(PAM);

Alternative:

Organism:

Rat (Rattus norvegicus).

General Annotation

Sub Unit:

Monomer. Interacts with RASSF9.

Function:

Bifunctional enzyme that catalyzes 2 sequential steps in C-terminal alpha-amidation of peptides. The monooxygenase part produces an unstable peptidyl(2-hydroxyglycine) intermediate that is dismutated to glyoxylate and the corresponding desglycine peptide amide by the lyase part. C-terminal amidation of peptides such as neuropeptides is essential for full biological activity.

Subcellular Location:

Cytoplasmic vesicle secretory vesicle membrane Single-pass membrane protein Secretory granules.

Protein Attributes:

Sequence length:

976

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

550:

600:

650:

700:

750:

800:

850:

900:

950:

976:

QEKDEDDGTE | SEEEYSAPLP | KPAPSS

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

SMR:

P14925

Pfam:

PF01082

UniGene:

Rn.1121

String:

P14925

KEGG:

rno:25508

Uniprot:

P14925

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ELISA Kit for Rat PAM

CLIA Kit for Rat PAM

Polyclonal Antibody for Rat PAM

Monoclonal Antibody for Rat PAM

Protein for Rat PAM

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Alternative mRNA splicing generates multiple forms of peptidyl-glycine alpha-amidating monooxygenase in rat atrium."
Stoffers D.A. , Green C.B.R. , Eipper B.A.
Proc. Natl. Acad. Sci. U.S.A.86:735-739(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (PAM-1/2)
strain: Sprague-Dawley.
tissue: Heart atrium.
2.

"Characterization of novel mRNAs encoding enzymes involved in peptide alpha-amidation."
Stoffers D.A. , Ouafik L. , Eipper B.A.
J. Biol. Chem.266:1701-1707(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (PAM-3/4)
strain: Sprague-Dawley.
tissue: Heart atrium.
3.

"Isolation and functional expression of pituitary peptidylglycine alpha-amidating enzyme mRNA. A variant lacking the transmembrane domain."
Kato I. , Yonekura H. , Yamamoto H. , Okamoto H.
FEBS Lett.269:319-323(1990) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (PAM-1 TO 5)
strain: Wistar.
tissue: Pituitary.
4.

"Cloning and characterization of two alternatively spliced rat alpha-amidating enzyme cDNAs from rat medullary thyroid carcinoma."
Bertelsen A.H. , Beaudry G.A. , Galella E.A. , Jones B.N. , Ray M.L. , Mehta N.M.
Arch. Biochem. Biophys.279:87-96(1990) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (PAM-4)
strain: Sprague-Dawley.
tissue: Liver.
5.

"Alternative splicing and endoproteolytic processing generate tissue-specific forms of pituitary peptidylglycine alpha-amidating monooxygenase (PAM)."
Eipper B.A. , Green C.B. , Campbell T.A. , Stoffers D.A. , Keutmann H.T. , Mains R.E. , Ouafik L.
J. Biol. Chem.267:4008-4015(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];ALTERNATIVE SPLICING
6.

"Purification and characterization of functional recombinant alpha-amidating enzyme secreted from mammalian cells."
Beaudry G.A. , Mehta N.M. , Ray M.L. , Bertelsen A.H.
J. Biol. Chem.265:17694-17699(1990) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 26-42;GLYCOSYLATION AT ASN-765
7.

"The multifunctional peptidylglycine alpha-amidating monooxygenase gene: exon/intron organization of catalytic, processing, and routing domains."
Ouafik L.H. , Stoffers D.A. , Campbell T.A. , Johnson R.C. , Bloomquist B.T. , Mains R.E. , Eipper B.A.
Mol. Endocrinol.6:1571-1584(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ALTERNATIVE SPLICING
8.

"P-CIP1, a novel protein that interacts with the cytosolic domain of peptidylglycine alpha-amidating monooxygenase, is associated with endosomes."
Chen L. , Johnson R.C. , Milgram S.L.
J. Biol. Chem.273:33524-33532(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH RASSF9
9.

"Essential features of the catalytic core of peptidyl-alpha-hydroxyglycine alpha-amidating lyase."
Kolhekar A.S. , Bell J. , Shiozaki E.N. , Jin L. , Keutmann H.T. , Hand T.A. , Mains R.E. , Eipper B.A.
Biochemistry41:12384-12394(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DISULFIDE BONDS IN CATALYTIC DOMAIN
10.

"Amidation of bioactive peptides: the structure of peptidylglycine alpha-hydroxylating monooxygenase."
Prigge S.T. , Kolhekar A.S. , Eipper B.A. , Mains R.E. , Amzel L.M.
Science278:1300-1305(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 45-354

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