AOPEP ELISA Kit for Human(Homo sapiens) | EIAab.comAOPEP ELISA Kit for Human(Homo sapiens)

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Index > Protein center > AOPEP(Gene name) > Human

AOPEP (Gene name),

Aminopeptidase O (Protein name ), AMPO_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

AOPEP(C9orf3;ONPEP);

Protein name:

Aminopeptidase O(AP-O);

Alternative:

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

N/A

Function:

Aminopeptidases catalyze the hydrolysis of amino acid residues from the N-terminus of peptide or protein substrates. Able to cleave angiotensin III to generate angiotensin IV, a bioactive peptide of the renin-angiotensin pathway. Not able to cleave angiotensin I and angiotensin II. May play a role in the proteolytic processing of bioactive peptides in tissues such as testis and heart.

Subcellular Location:

Cytoplasm

Protein Attributes:

Sequence length:

819

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

550:

600:

650:

700:

750:

800:

819:

RTKEQMDRSS | AQVVAEMLF

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

Pfam:

PF09127

String:

Q8N6M6

SMR:

Q8N6M6

UniGene:

Hs.434253

KEGG:

hsa:84909

STRING:

9606.ENSP00000297979

Uniprot:

Q8N6M6

FOR

ELISA Kit for Human AP-O

Cat.:

E12538h

Price:

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Manual:

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Packing:

96T

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ELISA Kit for Human AP-O

Cat.:

E12538m

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Packing:

96T

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ELISA Kit for Human AP-O

Cat.:

E12538r

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Packing:

96T

Range:

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CLIA Kit for Human AP-O

Cat.:

U12538r

Price:

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Manual:

Looking for manual.

MSDS:

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Packing:

96T

Range:

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CLIA Kit for Human AP-O

Cat.:

U12538m

Price:

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Manual:

Looking for manual.

MSDS:

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Packing:

96T

Range:

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CLIA Kit for Human AP-O

Cat.:

U12538h

Price:

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Manual:

Looking for manual.

MSDS:

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Packing:

96T

Range:

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Polyclonal Antibody for Human AP-O

Cat.:

P12538Rb-h

Price:

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Packing:

40ug/0.2ml

Monoclonal Antibody for Human AP-O

Protein for Human AP-O

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.4%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.1%

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.4%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of AMPO into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	103	96-105
Plasma	101	92-106

Recovery was determined by spiking various levels of AMPO into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	95	90-100
Plasma	96	91-102

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AMPO and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	98-107%	95-111%	97-109%	94-110%
EDTA plasma(n=5)	93-108%	97-105%	95-107%	96-106%
heparin plasma(n=5)	96-104%	94-109%	98-105%	97-107%

Sample	1：2	1:4	1:8	1:16
serum(n=5)	88-104%	91-98%	90-101%	92-98%
EDTA plasma(n=5)	90-103%	93-100%	88-105%	90-104%
heparin plasma(n=5)	92-102%	91-102%	92-101%	93-98%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

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