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Index > Protein center > Pmaip1(Gene name) > Mouse
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  • Pmaip1 (Gene name),
  • Phorbol-12-myristate-13-acetate-induced protein 1 (Protein name ),  APR_MOUSE from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Gene name:
    Pmaip1(Noxa);
    Protein name:
    Phorbol-12-myristate-13-acetate-induced protein 1;
    Alternative:
    Protein Noxa;
    Organism:
    Mouse (Mus musculus). 
    General Annotation
    Sub Unit:
    Interacts with MCL1 and BAX.
    Function:
    Promotes activation of caspases and apoptosis. Promotes mitochondrial membrane changes and efflux of apoptogenic proteins from the mitochondria. Contributes to p53/TP53-dependent apoptosis after radiation exposure. Promotes proteasomal degradation of MCL1. Competes with BIM/BCL2L11 for binding to MCL1 and can displace BIM/BCL2L11 from its binding site on MCL1 (By similarity). Competes with BAK1 for binding to MCL1 and can displace BAK1 from its binding site on MCL1.
    Subcellular Location:
    Mitochondrion
    Protein Attributes:
    Sequence length:
    103
    Sequence:
    50:
    MPGRKARRNA | PVNPTRAELP | PEFAAQLRKI | GDKVYCTWSA | PDITVVLAQM | 
    100:
    PGKSQKSRMR | SPSPTRVPAD | LKDECAQLRR | IGDKVNLRQK | LLNLISKLFN | 
    103:
    LVT
    3D Structure:
    N/A
    Predicted Eptitope:
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    EIAab Sequence  Vaild Sequence:
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    Related Databases
    SMR:
    Pfam:
    String:
    UniGene:
    KEGG:
    Uniprot:
     
    FOR
    ELISA Kit for Mouse Phorbol-12-myristate-13-acetate-induced protein 1
    Cat.:
    E13268r
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    Packing:
    96T
    ELISA Kit for Mouse Phorbol-12-myristate-13-acetate-induced protein 1
    Cat.:
    E13268m
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    MSDS:
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    Packing:
    96T
    ELISA Kit for Mouse Phorbol-12-myristate-13-acetate-induced protein 1
    Cat.:
    E13268h
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    Packing:
    96T
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    ELISA Kit for Mouse Phorbol-12-myristate-13-acetate-induced protein 1
    MSDS:
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    CLIA Kit for Mouse Phorbol-12-myristate-13-acetate-induced protein 1
    MSDS:
    Please sign in first.
    CLIA Kit for Mouse Phorbol-12-myristate-13-acetate-induced protein 1
    Cat.:
    U13268m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Mouse Phorbol-12-myristate-13-acetate-induced protein 1
    Cat.:
    U13268h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Mouse Phorbol-12-myristate-13-acetate-induced protein 1
    MSDS:
    Please sign in first.
    Polyclonal Antibody for Mouse Phorbol-12-myristate-13-acetate-induced protein 1
    Polyclonal Antibody for Mouse Phorbol-12-myristate-13-acetate-induced protein 1
    Polyclonal Antibody for Mouse Phorbol-12-myristate-13-acetate-induced protein 1
    Polyclonal Antibody for Mouse Phorbol-12-myristate-13-acetate-induced protein 1
    Monoclonal Antibody for Mouse Phorbol-12-myristate-13-acetate-induced protein 1
    Monoclonal Antibody for Mouse Phorbol-12-myristate-13-acetate-induced protein 1
    Monoclonal Antibody for Mouse Phorbol-12-myristate-13-acetate-induced protein 1
    Monoclonal Antibody for Mouse Phorbol-12-myristate-13-acetate-induced protein 1
    Protein for Mouse Phorbol-12-myristate-13-acetate-induced protein 1
    Protein for Mouse Phorbol-12-myristate-13-acetate-induced protein 1
    Protein for Mouse Phorbol-12-myristate-13-acetate-induced protein 1
    Protein for Mouse Phorbol-12-myristate-13-acetate-induced protein 1

    R&D Technical Data
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            12% SDS-PAGE Analysis

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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤ 6.1%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤ 11.3%

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    Recovery
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    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    92-104

    Plasma

    98

    94-107

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    91-101%

    96-105%

    93-106%

    EDTA plasma(n=5)

    89-97%

    92-104%

    89-99%

    92-107%

    heparin plasma(n=5)

    92-104%

     

    94-110%

    101-108%

    93-109%

     

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    References
    1. 1.
      "Noxa, a BH3-only member of the Bcl-2 family and candidate mediator of p53-induced apoptosis."
      Oda E. , Ohki R. , Murasawa H. , Nemoto J. , Shibue T. , Yamashita T. , Tokino T. , Taniguchi T. , Tanaka N.
      Science288:1053-1058(2000) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];INTERACTION WITH MCL1;SUBCELLULAR LOCATION;FUNCTION;MUTAGENESIS OF LEU-27 AND LEU-78
    2. 2.
      "The transcriptional landscape of the mammalian genome."
      Carninci P. , Kasukawa T. , Katayama S. , Gough J. , Frith M.C. , Maeda N. , Oyama R. , Ravasi T. , Lenhard B. , Wells C. , Kodzius R. , Shimokawa K. , Bajic V.B. , Brenner S.E. , Batalov S. , Forrest A.R. , Zavolan M. , Davis M.J. , more...
      Science309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      strain: C57BL/6J.
      strain: NOD.
      tissue: Brain cortex.
      tissue: Dendritic cell.
      tissue: Kidney.
      tissue: Thymus.
    3. 3.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      tissue: Limb.
    4. 4.
      "Proapoptotic Bak is sequestered by Mcl-1 and Bcl-xL, but not Bcl-2, until displaced by BH3-only proteins."
      Willis S.N. , Chen L. , Dewson G. , Wei A. , Naik E. , Fletcher J.I. , Adams J.M. , Huang D.C.S.
      Genes Dev.19:1294-1305(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH MCL1
    5. 5.
      "Differential targeting of prosurvival Bcl-2 proteins by their BH3-only ligands allows complementary apoptotic function."
      Chen L. , Willis S.N. , Wei A. , Smith B.J. , Fletcher J.I. , Hinds M.G. , Colman P.M. , Day C.L. , Adams J.M. , Huang D.C.S.
      Mol. Cell17:393-403(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH MCL1
    6. 6.
      "BH3-only proapoptotic Bcl-2 family members Noxa and Puma mediate neural precursor cell death."
      Akhtar R.S. , Geng Y. , Klocke B.J. , Latham C.B. , Villunger A. , Michalak E.M. , Strasser A. , Carroll S.L. , Roth K.A.
      J. Neurosci.26:7257-7264(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INDUCTION;TISSUE SPECIFICITY
    7. 7.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 68-93 IN COMPLEX WITH MCL1;STRUCTURE BY NMR OF 68-94 IN COMPLEX WITH MCL1;FUNCTION;INTERACTION WITH MCL1;IDENTIFICATION BY MASS SPECTROMETRY
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