AREG ELISA Kit for Human(Homo sapiens) | EIAab.comAREG ELISA Kit for Human(Homo sapiens) | EIAab.com

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AREG (Gene name),

Amphiregulin (Protein name ), AREG_HUMAN from NCBI database.

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General Annotation

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Antigen Annotation

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3D

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Predicted Eptitope

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Vaild Sequence

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Related Databases

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Gene name:

AREG(SDGF);

Protein name:

Amphiregulin(AR);

Alternative:

Colorectum cell-derived growth factor(CRDGF);

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

The immature precursor interacts with CNIH.

Function:

Bifunctional growth-modulating glycoprotein. Inhibits growth of several human carcinoma cells in culture and stimulates proliferation of human fibroblasts and certain other tumor cells.

Subcellular Location:

Membrane Single-pass membrane protein

Protein Attributes:

Sequence length:

252

Sequence:

50:

MRAPLLPPAP | VVLSLLILGS | GHYAAGLDLN | DTYSGKREPF | SGDHSADGFE |

100:

VTSRSEMSSG | SEISPVSEMP | SSSEPSSGAD | YDYSEEYDNE | PQIPGYIVDD |

150:

SVRVEQVVKP | PQNKTESENT | SDKPKRKKKG | GKNGKNRRNR | KKKNPCNAEF |

200:

QNFCIHGECK | YIEHLEAVTC | KCQQEYFGER | CGEKSMKTHS | MIDSSLSKIA |

250:

LAAIAAFMSA | VILTAVAVIT | VQLRRQYVRK | YEGEAEERKK | LRQENGNVHA |

252:

IA

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

MIM:

STRING:

9606.ENSP00000264487

String:

UniGene:

KEGG:

SMR:

Uniprot:

FOR

ELISA Kit for Human AR

Cat.:

E0006m

Price:

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MSDS:

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Packing:

96T

Range:

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ELISA Kit for Human AR

Cat.:

E0006h

Price:

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Manual:

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MSDS:

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Packing:

96T

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ELISA Kit for Human AR

Cat.:

E0006r

Price:

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MSDS:

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Packing:

96T

CLIA Kit for Human AR

Cat.:

U0006h

Price:

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Manual:

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MSDS:

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Packing:

96T

Range:

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CLIA Kit for Human AR

Cat.:

U0006r

Price:

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MSDS:

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Packing:

96T

CLIA Kit for Human AR

Cat.:

U0006m

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96T

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Polyclonal Antibody for Human AR

Cat.:

P0006Rb-r

Price:

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human AR

Cat.:

P0006Rb-h

Price:

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human AR

Cat.:

P0006Rb-m

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Packing:

40ug/0.2ml

Monoclonal Antibody for Human AR

Monoclonal Antibody for Human AR

Monoclonal Antibody for Human AR

Protein for Human AR

Protein for Human AR

Protein for Human AR

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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Precision

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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.2%

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Recovery

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Recovery was determined by spiking various levels of LRP8 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	94-105
Plasma	98	93-104

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Linearity

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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LRP8 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	94-105%	97-103%	95-104%	92-109%
EDTA plasma(n=5)	92-99%	93-105%	93-101%	96-106%
heparin plasma(n=5)	93-105%	96-110%	98-109%	98-110%

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References

1.

"The amphiregulin gene encodes a novel epidermal growth factor-related protein with tumor-inhibitory activity."
Plowman G.D. , Green J.M. , McDonald V.L. , Neubauer M.G. , Disteche C.M. , Todaro G.J. , Shoyab M.
Mol. Cell. Biol.10:1969-1981(1990) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]
2.

NIEHS SNPs program
Submitted (2003-10) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANTS VAL-80 AND CYS-81
3.

"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N. , Chen X. , Rolfs A. , Halleck A. , Hines L. , Eisenstein S. , Koundinya M. , Raphael J. , Moreira D. , Kelley T. , LaBaer J. , Lin Y. , Phelan M. , Farmer A.
Submitted (2004-10) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
4.

Mural R.J. , Istrail S. , Sutton G. , Florea L. , Halpern A.L. , Mobarry C.M. , Lippert R. , Walenz B. , Shatkay H. , Dew I. , Miller J.R. , Flanigan M.J. , Edwards N.J. , Bolanos R. , Fasulo D. , Halldorsson B.V. , Hannenhalli S. , Turner R. , more...
Submitted (2005-07) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
5.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
tissue: Brain.
6.

"Structure and function of human amphiregulin: a member of the epidermal growth factor family."
Shoyab M. , Plowman G.D. , McDonald V.L. , Bradley J.G. , Todaro G.J.
Science243:1074-1076(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 101-184
7.

"Amphiregulin: a bifunctional growth-modulating glycoprotein produced by the phorbol 12-myristate 13-acetate-treated human breast adenocarcinoma cell line MCF-7."
Shoyab M. , McDonald V.L. , Bradley J.G. , Todaro G.J.
Proc. Natl. Acad. Sci. U.S.A.85:6528-6532(1988) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 101-131
8.

"A heparin sulfate-regulated human keratinocyte autocrine factor is similar or identical to amphiregulin."
Cook P.W. , Mattox P.A. , Keeble W.W. , Pittelkow M.R. , Plowman G.D. , Shoyab M. , Adelman J.P. , Shipley G.D.
Mol. Cell. Biol.11:2547-2557(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 101-126
9.

"Colorectum cell-derived growth factor (CRDGF) is homologous to amphiregulin, a member of the epidermal growth factor family."
Culouscou J.-M. , Remacle-Bonnet M. , Carlton G.W. , Plowman G.D. , Shoyab M.
Growth Factors7:195-205(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 101-122
10.

"Clarification of the C-terminal proteolytic processing site of human Amphiregulin."
Levano K.S. , Kenny P.A.
FEBS Lett.586:3500-3502(2012) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 188-194;CLEAVAGE SITE
11.

"Amphiregulin induces tyrosine phosphorylation of the epidermal growth factor receptor and p185erbB2. Evidence that amphiregulin acts exclusively through the epidermal growth factor receptor at the surface of human epithelial cells."
Johnson G.R. , Kannan B. , Shoyab M. , Stromberg K.
J. Biol. Chem.268:2924-2931(1993) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION AS EGFR LIGAND
12.

"Cornichon regulates transport and secretion of TGFalpha-related proteins in metazoan cells."
Perez Castro C. , Piscopo D. , Nakagawa T. , Derynck R.
J. Cell Sci.120:2454-2466(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH CNIH
13.

"Solution structure of the EGF-like domain from human amphiregulin."
RIKEN structural genomics initiative (RSGI)
Submitted (2009-01) to the PDB data bank

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 142-184;DISULFIDE BONDS

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