Asah2 ELISA Kit for Rat(Rattus norvegicus) | EIAab.comAsah2 ELISA Kit for Rat(Rattus norvegicus)

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Asah2 (Gene name),

Neutral ceramidase (Protein name ), ASAH2_RAT from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

Asah2;

Protein name:

Neutral ceramidase(N-CDase;NCDase);

Alternative:

N-acylsphingosine amidohydrolase 2;Acylsphingosine deacylase 2;

Organism:

Rat (Rattus norvegicus).

General Annotation

Sub Unit:

N/A

Function:

Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract.

Subcellular Location:

Cell membrane Single-pass type II membrane protein The neutral ceramidase soluble form is a secreted protein.

Protein Attributes:

Sequence length:

761

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

550:

600:

650:

700:

750:

761:

GISSPFEIVT | T

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

String:

Q91XT9

UniGene:

Rn.156958

KEGG:

rno:114104

Pfam:

PF04734

STRING:

10116.ENSRNOP00000016688

Uniprot:

Q91XT9

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ELISA Kit for Rat N-CDase

Cat.:

E11205m

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96T

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ELISA Kit for Rat N-CDase

Cat.:

E11205r

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96T

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ELISA Kit for Rat N-CDase

Cat.:

E11205h

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96T

CLIA Kit for Rat N-CDase

Cat.:

U11205r

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96T

CLIA Kit for Rat N-CDase

Cat.:

U11205h

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96T

CLIA Kit for Rat N-CDase

Cat.:

U11205m

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Packing:

96T

Polyclonal Antibody for Rat N-CDase

Cat.:

P11205Rb-h

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40ug/0.2ml

Polyclonal Antibody for Rat N-CDase

Cat.:

P11205Rb-r

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40ug/0.2ml

Polyclonal Antibody for Rat N-CDase

Cat.:

P11205Rb-m

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40ug/0.2ml

Monoclonal Antibody for Rat N-CDase

Protein for Rat N-CDase

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

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Linearity

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References

1.

"Purification, characterization, molecular cloning, and subcellular distribution of neutral ceramidase of rat kidney."
Mitsutake S. , Tani M. , Okino N. , Mori K. , Ichinose S. , Omori A. , Iida H. , Nakamura T. , Ito M.
J. Biol. Chem.276:26249-26259(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];PROTEIN SEQUENCE OF 223-245; 261-273; 601-619 AND 701-729;ENZYME ACTIVITY;BIOPHYSICOCHEMICAL PROPERTIES;GLYCOSYLATION;SUBCELLULAR LOCATION;TISSUE SPECIFICITY
tissue: Kidney.
2.

"O-glycosylation of mucin-like domain retains the neutral ceramidase on the plasma membranes as a type II integral membrane protein."
Tani M. , Iida H. , Ito M.
J. Biol. Chem.278:10523-10530(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 80-100;SUBCELLULAR LOCATION;TOPOLOGY;GLYCOSYLATION;MUTAGENESIS OF SER-44; 50-SER--THR-71 AND SER-77
3.

"Molecular cloning and characterization of a human mitochondrial ceramidase."
El Bawab S. , Roddy P. , Qian T. , Bielawska A. , Lemasters J.J. , Hannun Y.A.
J. Biol. Chem.275:21508-21513(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 311-327; 635-648 AND 737-753
4.

"Purification and characterization of a membrane-bound nonlysosomal ceramidase from rat brain."
El Bawab S. , Bielawska A. , Hannun Y.A.
J. Biol. Chem.274:27948-27955(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ENZYME ACTIVITY;BIOPHYSICOCHEMICAL PROPERTIES
5.

"Distribution and properties of neutral ceramidase activity in rat intestinal tract."
Lundgren P. , Nilsson A. , Duan R.D.
Dig. Dis. Sci.46:765-772(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: TISSUE SPECIFICITY
6.

"Interleukin-1beta induces chronic activation and de novo synthesis of neutral ceramidase in renal mesangial cells."
Franzen R. , Pautz A. , Braeutigam L. , Geisslinger G. , Pfeilschifter J. , Huwiler A.
J. Biol. Chem.276:35382-35389(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INDUCTION
7.

"Nitric oxide induces degradation of the neutral ceramidase in rat renal mesangial cells and is counterregulated by protein kinase C."
Franzen R. , Fabbro D. , Aschrafi A. , Pfeilschifter J. , Huwiler A.
J. Biol. Chem.277:46184-46190(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION
8.

"Nitric oxide induces neutral ceramidase degradation by the ubiquitin/proteasome complex in renal mesangial cell cultures."
Franzen R. , Pfeilschifter J. , Huwiler A.
FEBS Lett.532:441-444(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: UBIQUITINATION
9.

"Conserved amino acid residues in the COOH-terminal tail are indispensable for the correct folding and localization and enzyme activity of neutral ceramidase."
Tani M. , Okino N. , Sueyoshi N. , Ito M.
J. Biol. Chem.279:29351-29358(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: MUTAGENESIS OF PHE-756; GLU-757 AND ILE-758
10.

"Rat intestinal ceramidase: purification, properties, and physiological relevance."
Olsson M. , Duan R.-D. , Ohlsson L. , Nilsson A.
Am. J. Physiol.287:G929-G937(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY;FUNCTION;BIOPHYSICOCHEMICAL PROPERTIES;GLYCOSYLATION

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