BANF1 ELISA Kit for Human(Homo sapiens) | EIAab.comBANF1 ELISA Kit for Human(Homo sapiens)

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BANF1 (Gene name),

Barrier-to-autointegration factor (Protein name ), BAF_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

BANF1(BAF;BCRG1);

Protein name:

Barrier-to-autointegration factor;

Alternative:

Breakpoint cluster region protein 1;

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Homodimer. Heterodimerizes with BAFL. Binds non-specifically to double-stranded DNA, and is found as a hexamer or dodecamer upon DNA binding. Binds to LEM domain-containing nuclear proteins such as LEMD3/MAN1, TMPO/LAP2 and EMD (emerin). Interacts with CRX and LMNA (lamin-A). Binds linker histone H1.1 and core histones H3 with in vitro affinities of 500-900 and 100-200 nM. Interacts with HIV-1 pre-integration complex in cytoplasm by binding to viral matrix protein and Gag polyprotein.

Function:

Plays fundamental roles in nuclear assembly, chromatin organization, gene expression and gonad development. May potently compress chromatin structure and be involved in membrane recruitment and chromatin decondensation during nuclear assembly. Contains 2 non-specific dsDNA-binding sites which may promote DNA cross-bridging. Exploited by retroviruses for inhibiting self-destructing autointegration of retroviral DNA, thereby promoting integration of viral DNA into the host chromosome. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD.

Subcellular Location:

Nucleus Cytoplasm Chromosome Significantly enriched at the nuclear inner membrane, diffusely throughout the nucleus during interphase and concentrated at the chromosomes during the M-phase. May be included in HIV-1 virions via its interaction with viral GAG polyprotein. The phosphorylated form (by VRK1) shows a cytoplasmic localization.

Protein Attributes:

Sequence length:

Sequence:

50:

89:

VLKKDEDLFR | EWLKDTCGAN | AKQSRDCFGC | LREWCDAFL

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

UniGene:

Hs.433759

MIM:

603811

Pfam:

PF02961

String:

O75531

KEGG:

hsa:8815

STRING:

9606.ENSP00000310275

SMR:

O75531

Uniprot:

O75531

FOR

ELISA Kit for Human Barrier-to-autointegration factor

CLIA Kit for Human Barrier-to-autointegration factor

Polyclonal Antibody for Human Barrier-to-autointegration factor

Monoclonal Antibody for Human Barrier-to-autointegration factor

Protein for Human Barrier-to-autointegration factor

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"A previously unidentified host protein protects retroviral DNA from autointegration."
Lee M.S. , Craigie R.
Proc. Natl. Acad. Sci. U.S.A.95:1528-1533(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
2.

"Genomic and functional map of the chromosome 14 t(12;14) breakpoint cluster region in uterine leiomyoma."
Lynch R.A. , Piper M. , Bankier A. , Bhugra B. , Surti U. , Liu J. , Buckler A. , Dear P.H. , Menon A.G.
Genomics52:17-26(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
tissue: Uterus.
3.

"Human BAF homolog gene."
Zhang J. , Liu T. , Ye M. , Zhang Q. , Fu G. , Zhou J. , Wu J. , Shen Y. , Yu M. , Chen S. , Mao M. , Chen Z.
Submitted (1998-05) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
4.

"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L. , Schick M. , Neubert P. , Schatten R. , Henze S. , Korn B.
Submitted (2004-06) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
5.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
tissue: Brain.
6.

"Both the structure and DNA binding function of the barrier-to-autointegration factor contribute to reconstitution of HIV type 1 integration in vitro."
Harris D. , Engelman A.
J. Biol. Chem.275:39671-39677(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN HIV-1 INTEGRATION;MUTAGENESIS OF LYS-6; PRO-14; LYS-18; ILE-26; LYS-41; LEU-50; LYS-53; LYS-54; TRP-62 AND CYS-80
7.

"Barrier-to-autointegration factor (BAF) bridges DNA in a discrete, higher-order nucleoprotein complex."
Zheng R. , Ghirlando R. , Lee M.S. , Mizuuchi K. , Krause M. , Craigie R.
Proc. Natl. Acad. Sci. U.S.A.97:8997-9002(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: MULTIMERIZATION
8.

"BAF is required for emerin assembly into the reforming nuclear envelope."
Haraguchi T. , Koujin T. , Segura-Totten M. , Lee K.K. , Matsuoka Y. , Yoneda Y. , Wilson K.L. , Hiraoka Y.
J. Cell Sci.114:4575-4585(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH EMD
9.

"Barrier-to-autointegration factor: major roles in chromatin decondensation and nuclear assembly."
Segura-Totten M. , Kowalski A.K. , Craigie R. , Wilson K.L.
J. Cell Biol.158:475-485(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;MUTAGENESIS OF LYS-6; ASP-9; GLY-25; GLY-27; LEU-46; GLY-47; VAL-51; LYS-53; LYS-54 AND ARG-75
10.

"The barrier-to-autointegration factor is a component of functional human immunodeficiency virus type 1 preintegration complexes."
Lin C.W. , Engelman A.
J. Virol.77:5030-5036(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH HIV-1 PRE-INTEGRATION COMPLEX
11.

"Barrier-to-autointegration factor BAF binds p55 Gag and matrix and is a host component of human immunodeficiency virus type 1 virions."
Mansharamani M. , Graham D.R. , Monie D. , Lee K.K. , Hildreth J.E. , Siliciano R.F. , Wilson K.L.
J. Virol.77:13084-13092(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH HIV-1 MATRIX PROTEIN
12.

"Direct binding of nuclear membrane protein MAN1 to emerin in vitro and two modes of binding to barrier-to-autointegration factor."
Mansharamani M. , Wilson K.L.
J. Biol. Chem.280:13863-13870(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH LEMD3/MAN1;MUTAGENESIS OF LYS-6; ARG-8; ASP-9; PRO-14; GLY-25; ILE-26; ARG-37; LYS-41; LEU-46; GLY-47; LEU-50; VAL-51; LYS-53; LYS-54; TRP-62 AND CYS-80
13.

"Binding of barrier to autointegration factor (BAF) to histone H3 and selected linker histones including H1.1."
de Oca R.M. , Lee K.K. , Wilson K.L.
J. Biol. Chem.280:42252-42262(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH HISTONE H1/H3;MUTAGENESIS OF LYS-6; ARG-8; ASP-9; PRO-14; LYS-18; GLY-25; ILE-26; VAL-29; LYS-32; LYS-33; ARG-37; LYS-41; LEU-46; GLY-47; LEU-50; VAL-51; LYS-53; LYS-54; ARG-60; TRP-62; LYS-64; ARG-75; CYS-80 AND ARG-82
14.

"Barrier-to-autointegration factor phosphorylation on Ser-4 regulates emerin binding to lamin A in vitro and emerin localization in vivo."
Bengtsson L. , Wilson K.L.
Mol. Biol. Cell17:1154-1163(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT SER-4;MUTAGENESIS OF SER-4
15.

"Barrier-to-autointegration factor-like (BAF-L): a proposed regulator of BAF."
Tifft K.E. , Segura-Totten M. , Lee K.K. , Wilson K.L.
Exp. Cell Res.312:478-487(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH BAFL
16.

"The vaccinia-related kinases phosphorylate the N' terminus of BAF, regulating its interaction with DNA and its retention in the nucleus."
Nichols R.J. , Wiebe M.S. , Traktman P.
Mol. Biol. Cell17:2451-2464(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT THR-2; THR-3 AND SER-4;SUBCELLULAR LOCATION
17.

"The inner-nuclear-envelope protein emerin regulates HIV-1 infectivity."
Jacque J.-M. , Stevenson M.
Nature441:641-645(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
18.

"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S. , Helbig A.O. , Slijper M. , Krijgsveld J. , Heck A.J. , Mohammed S.
Anal. Chem.81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT THR-2;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS];CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS]
19.

"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Choudhary C. , Kumar C. , Gnad F. , Nielsen M.L. , Rehman M. , Walther T.C. , Olsen J.V. , Mann M.
Science325:834-840(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
20.

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V. , Vermeulen M. , Santamaria A. , Kumar C. , Miller M.L. , Jensen L.J. , Gnad F. , Cox J. , Jensen T.S. , Nigg E.A. , Brunak S. , Mann M.
Sci. Signal.3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
21.

"Initial characterization of the human central proteome."
Burkard T.R. , Planyavsky M. , Kaupe I. , Breitwieser F.P. , Buerckstuemmer T. , Bennett K.L. , Superti-Furga G. , Colinge J.
BMC Syst. Biol.5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
22.

"Coordination of kinase and phosphatase activities by Lem4 enables nuclear envelope reassembly during mitosis."
Asencio C. , Davidson I.F. , Santarella-Mellwig R. , Ly-Hartig T.B. , Mall M. , Wallenfang M.R. , Mattaj I.W. , Gorjanacz M.
Cell150:122-135(2012) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION;DEPHOSPHORYLATION;INTERACTION WITH ANKLE2
23.

"BAF: roles in chromatin, nuclear structure and retrovirus integration."
Segura-Totten M. , Wilson K.L.
Trends Cell Biol.14:261-266(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW
24.

"Solution structure of the cellular factor BAF responsible for protecting retroviral DNA from autointegration."
Cai M. , Huang Y. , Zheng R. , Wei S.Q. , Ghirlando R. , Lee M.S. , Craigie R. , Gronenborn A.M. , Clore G.M.
Nat. Struct. Biol.5:903-909(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR
25.

"Structural basis of DNA bridging by barrier-to-autointegration factor."
Umland T.C. , Wei S.-Q. , Craigie R. , Davies D.R.
Biochemistry39:9130-9138(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF HOMODIMER
26.

"Exome sequencing and functional analysis identifies BANF1 mutation as the cause of a hereditary progeroid syndrome."
Puente X.S. , Quesada V. , Osorio F.G. , Cabanillas R. , Cadinanos J. , Fraile J.M. , Ordonez G.R. , Puente D.A. , Gutierrez-Fernandez A. , Fanjul-Fernandez M. , Levy N. , Freije J.M. , Lopez-Otin C.
Am. J. Hum. Genet.88:650-656(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT NGPS THR-12

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