BCO2 ELISA Kit for Human(Homo sapiens) | EIAab.comBCO2 ELISA Kit for Human(Homo sapiens) | EIAab.com

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BCO2 (Gene name),

Beta,beta-carotene 9',10'-oxygenase (Protein name ), BCDO2_HUMAN from NCBI database.

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General Annotation

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Antigen Annotation

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3D

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Predicted Eptitope

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Vaild Sequence

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Gene name:

BCO2(BCDO2);

Protein name:

Beta,beta-carotene 9',10'-oxygenase;

Alternative:

Beta-carotene dioxygenase 2;B-diox-II;

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

N/A

Function:

Asymmetrically cleaves beta-carotene at the 9',10' double bond resulting in the formation of beta-apo-10'-carotenal and beta-ionone. Besides beta-carotene, lycopene is also oxidatively cleaved. The apocarotenals formed by this enzyme may be the precursors for the biosynthesis of retinoic acid or exert unknown physiological effects.

Subcellular Location:

N/A

Protein Attributes:

Sequence length:

579

Sequence:

50:

MFFRVFLHFI | RSHSATAVDF | LPVMVHRLPV | FKRYMGNTPQ | KKAVFGQCRG |

100:

LPCVAPLLTT | VEEAPRGISA | RVWGHFPKWL | NGSLLRIGPG | KFEFGKDKYN |

150:

HWFDGMALLH | QFRMAKGTVT | YRSKFLQSDT | YKANSAKNRI | VISEFGTLAL |

200:

PDPCKNVFER | FMSRFELPGK | AAAMTDNTNV | NYVRYKGDYY | LCTETNFMNK |

250:

VDIETLEKTE | KVDWSKFIAV | NGATAHPHYD | LDGTAYNMGN | SFGPYGFSYK |

300:

VIRVPPEKVD | LGETIHGVQV | ICSIASTEKG | KPSYYHSFGM | TRNYIIFIEQ |

350:

PLKMNLWKIA | TSKIRGKAFS | DGISWEPQCN | TRFHVVEKRT | GQLLPGRYYS |

400:

KPFVTFHQIN | AFEDQGCVII | DLCCQDNGRT | LEVYQLQNLR | KAGEGLDQVH |

450:

NSAAKSFPRR | FVLPLNVSLN | APEGDNLSPL | SYTSASAVKQ | ADGTIWCSHE |

500:

NLHQEDLEKE | GGIEFPQIYY | DRFSGKKYHF | FYGCGFRHLV | GDSLIKVDVV |

550:

NKTLKVWRED | GFYPSEPVFV | PAPGTNEEDG | GVILSVVITP | NQNESNFILV |

579:

LDAKNFEELG | RAEVPVQMPY | GFHGTFIPI

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

KEGG:

String:

SMR:

UniGene:

Pfam:

MIM:

Uniprot:

FOR

ELISA Kit for Human Beta,beta-carotene 9',10'-oxygenase

Cat.:

E10866h

Price:

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Manual:

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MSDS:

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Packing:

96T

Range:

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ELISA Kit for Human Beta,beta-carotene 9',10'-oxygenase

Cat.:

E10866m

Price:

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MSDS:

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Packing:

96T

CLIA Kit for Human Beta,beta-carotene 9',10'-oxygenase

Cat.:

U10866h

Price:

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Manual:

Looking for manual.

MSDS:

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Packing:

96T

Range:

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CLIA Kit for Human Beta,beta-carotene 9',10'-oxygenase

Cat.:

U10866m

Price:

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MSDS:

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Packing:

96T

Polyclonal Antibody for Human Beta,beta-carotene 9',10'-oxygenase

Cat.:

P10866Rb-h

Price:

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human Beta,beta-carotene 9',10'-oxygenase

Monoclonal Antibody for Human Beta,beta-carotene 9',10'-oxygenase

Monoclonal Antibody for Human Beta,beta-carotene 9',10'-oxygenase

Protein for Human Beta,beta-carotene 9',10'-oxygenase

Protein for Human Beta,beta-carotene 9',10'-oxygenase

R&D Technical Data

s

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

s

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.9%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of BCDO2 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	92	88-99
Plasma	94	86-100

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BCDO2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	85-100%	83-101%	87-99%	89-97%
EDTA plasma(n=5)	89-100%	86-103%	89-101%	90-96%
heparin plasma(n=5)	90-98%	88-99%	91-97%	87-103%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"An ortholog of the human retinal pigment epithelium protein RPE65."
Cunningham F.X. Jr.
Submitted (2000-06) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2);VARIANTS PRO-231 AND LEU-548
tissue: Uterus.
2.

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 4 AND 6);VARIANTS PRO-231 AND LEU-548
tissue: Heart.
tissue: Placenta.
3.

NHLBI resequencing and genotyping service (RS&G)
Submitted (2007-02) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
4.

"Human chromosome 11 DNA sequence and analysis including novel gene identification."
Taylor T.D. , Noguchi H. , Totoki Y. , Toyoda A. , Kuroki Y. , Dewar K. , Lloyd C. , Itoh T. , Takeda T. , Kim D.-W. , She X. , Barlow K.F. , Bloom T. , Bruford E. , Chang J.L. , Cuomo C.A. , Eichler E. , FitzGerald M.G. , more...
Nature440:497-500(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
5.

Mural R.J. , Istrail S. , Sutton G. , Florea L. , Halpern A.L. , Mobarry C.M. , Lippert R. , Walenz B. , Shatkay H. , Dew I. , Miller J.R. , Flanigan M.J. , Edwards N.J. , Bolanos R. , Fasulo D. , Halldorsson B.V. , Hannenhalli S. , Turner R. , more...
Submitted (2005-07) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA];VARIANT LEU-548
6.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5);VARIANT LEU-548
tissue: Brain.
7.

"Identification and characterization of a mammalian enzyme catalyzing the asymmetric oxidative cleavage of provitamin A."
Kiefer C. , Hessel S. , Lampert J.M. , Vogt K. , Lederer M.O. , Breithaupt D.E. , von Lintig J.
J. Biol. Chem.276:14110-14116(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 17-579 (ISOFORM 1);VARIANTS PRO-231 AND LEU-548
tissue: Liver.
8.

"Cell type-specific expression of beta-carotene 9',10'-monooxygenase in human tissues."
Lindqvist A. , He Y.-G. , Andersson S.
J. Histochem. Cytochem.53:1403-1412(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: TISSUE SPECIFICITY
9.

"A mitochondrial enzyme degrades carotenoids and protects against oxidative stress."
Amengual J. , Lobo G.P. , Golczak M. , Li H.N. , Klimova T. , Hoppel C.L. , Wyss A. , Palczewski K. , von Lintig J.
FASEB J.25:948-959(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;SUBCELLULAR LOCATION

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