Protein name:
Brefeldin A-inhibited guanine nucleotide-exchange protein 1 (BIG1) ;
Alternative:
ADP-ribosylation factor guanine nucleotide-exchange factor 1 ;
Organism:
Mouse (Mus musculus).
General Annotation
Sub Unit:
Homodimer. Interacts with BIG1; both proteins are probably part of the same or very similar macromolecular complexes. Interacts with FKBP2, DPY30, MYO9B, PRKAR1A, PRKAR2A, PPP1CC and PDE3A. Interacts with KANK1; however, colocalization cannot be experimentally confirmed. Interacts with NCL, FBL, NUP62 and U3 small nucleolar RNA.
Function:
Promotes guanine-nucleotide exchange on ARF1 and ARF3. Promotes the activation of ARF1/ARF3 through replacement of GDP with GTP. Involved in vesicular trafficking. Required for the maintenance of Golgi structure; the function may be independent of its GEF activity. Required for the maturaion of integrin beta-1 in the Golgi. Involved in the establishment and persistence of cell polarity during directed cell movement in wound healing. Proposed to act as A kinase-anchoring protein (AKAP) and may mediate crosstalk between Arf and PKA pathways. Inhibits GAP activity of MYO9B probably through competetive RhoA binding. The function in the nucleus remains to be determined.
Subcellular Location:
Cytoplasm
Cytoplasm
Perinuclear region
Golgi apparatus
Golgi apparatus
Trans-Golgi network
Nucleus
Nucleus
Nucleolus
Nucleus matrix
Membrane
Translocates from cytoplasm to membranes and nucleus upon cAMP treatment.
Protein Attributes:
50:
MYEGKKTKNM | FLTRALEKIL | ADKEVKKAHH | SQLRKACEVA | LEEIKVETEK |
100:
QSPPHGEAKA | GSGTLPPVKS | KTNFIEADKY | FLPFELACQS | KCPRIVSTSL |
150:
DCLQKLIAYG | HLTGRAPDST | TPGKKLIDRI | IETICGCFQG | PQTDEGVQLQ |
200:
IIKALLTAVT | SQHIEIHEGT | VLQAVRTCYN | IYLASKNLIN | QTTAKATLTQ |
250:
MLNVIFARME | NQALQEAKQM | ERERHRQQQH | LLQSPVSHHE | PESPHLRYLP |
300:
PQTVDHINQE | HEGDLEPQTH | DVDKSLQDDT | EPENGSDISS | AENEQTEADQ |
350:
ATAAETLSKN | DILYDGDYEE | KPLDIVQSIV | EEMVNIIVGD | MGEGMAISAS |
400:
TEGNTGTVED | GSDSENIQAN | GIPGTPISVA | YTPSLPDDRL | SVSSNDTQES |
450:
GNSSGPSPGA | KFSHILQKDA | FLVFRSLCKL | SMKPLSDGPP | DPKSHELRSK |
500:
ILSLQLLLSI | LQNAGPVFRT | NEMFINAIKQ | YLCVALSKNG | VSSVPEVFEL |
550:
SLSIFLTLLS | NFKTHLKMQI | EVFFKEIFLY | ILETSTSSFD | HKWMVIQTLT |
600:
RICADAQSVV | DIYVNYDCDL | NAANIFERLV | NDLSKIAQGR | GSQELGMSNV |
650:
QELSLRKKGL | ECLVSILKCM | VEWSKDQYVN | PNSQTTLGQE | KPSEQEISEV |
700:
KHPETINRYG | SLNSLESTSS | SGIGSYSTQM | SGTDNPEQFE | VLKQQKEIIE |
750:
QGIDLFNKKP | KRGIQYLQEQ | GMLGTTPEDI | AQFLHQEERL | DSTQVGEFLG |
800:
DNDKFNKEVM | YAYVDQHDFS | GKDFVSALRL | FLEGFRLPGE | AQKIDRLMEK |
850:
FAARYLECNQ | GQTLFASADT | AYVLAYSIIM | LTTDLHSPQV | KNKMTKEQYI |
900:
KMNRGINDSK | DLPEEYLSAI | YNEIAGKKIS | MKETKELTIP | TKSTKQNVAS |
950:
EKQRRLLYNL | EMEQMAKTAK | ALMEAVSHVQ | APFTSATHLE | HVRPMFKLAW |
1000:
TPFLAAFSVG | LQDCDDTEVA | SLCLEGIRCA | IRIACIFSIQ | LERDAYVQAL |
1050:
ARFTLLTVSS | GITEMKQKNI | DTIKTLITVA | HTDGNYLGNS | WHEILKCISQ |
1100:
LELAQLIGTG | VKPRYISGTV | RGREGSLTGT | KDQAPDEFVG | LGLVGGNVDW |
1150:
KQIASIQESI | GETSSQSVVV | AVDRIFTGST | RLDGNAIVDF | VRWLCAVSMD |
1200:
ELLSTTHPRM | FSLQKIVEIS | YYNMGRIRLQ | WSRIWEVIGD | HFNKVGCNPN |
1250:
EDVAIFAVDS | LRQLSMKFLE | KGELANFRFQ | KDFLRPFEHI | MKRNRSPTIR |
1300:
DMVVRCIAQM | VNSQAANIRS | GWKNIFSVFH | LAASDQDESI | VELAFQTTGH |
1350:
IVTLVFEKHF | PATIDSFQDA | VKCLSEFACN | AAFPDTSMEA | IRLIRHCAKY |
1400:
VSDRPQAFKE | YTSDDMSVAP | EDRVWVRGWF | PILFELSCII | NRCKLDVRTR |
1450:
GLTVMFEIMK | TYGHTYEKHW | WQDLFRIVFR | IFDNMKLPEQ | QTEKAEWMTT |
1500:
TCNHALYAIC | DVFTQYLEVL | SDVLLDDIFA | QLYWCVQQDN | EQLARSGTNC |
1550:
LENVVILNGE | KFTLEIWDKT | CNCTLDIFKT | TIPHALLTWR | PTSGEAEPPS |
1600:
PSAVSEKPLD | AISQKSVDIH | DSIQPRSSDN | RQQAPLVSVS | TVSEEVSKVK |
1650:
STAKFPEQKL | FAALLIKCVV | QLELIQTIDN | IVFFPATSKK | EDAENLAAAQ |
1700:
RDAVDFDVRV | DTQDQGMYRF | LTSQQLFKLL | DCLLESHRFA | KAFNSNNEQR |
1750:
TALWKAGFKG | KSKPNLLKQE | TSSLACGLRI | LFRMYMDESR | VSAWEEVQQR |
1800:
LLNVCREALS | YFLTLTSESH | REAWTNLLLL | FLTKVLKISD | SRFKAHASFY |
1846:
YPLLCEIMQF | DLIPELRAVL | RRFFLRIGIV | FQISQPPEQE | LGINRQ
Vaild Sequence:
Related Databases
Uniprot:
ELISA Kit
CLIA Kit
Polyclonal Antibody
Monoclonal Antibody
Protein
FOR
Mouse
Rat
Human
Bovine
Polyclonal Antibody for Mouse BIG1
Polyclonal Antibody for Mouse BIG1
Polyclonal Antibody for Mouse BIG1
Polyclonal Antibody for Mouse BIG1
Monoclonal Antibody for Mouse BIG1
Monoclonal Antibody for Mouse BIG1
Monoclonal Antibody for Mouse BIG1
Monoclonal Antibody for Mouse BIG1
R&D Technical Data
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Precision
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Recovery
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Linearity
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References
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Europe PMC ]
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Abstract ]
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
strain :
C57BL/6J .
2.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
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Science309:1559-1563(2005)
[
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[
Europe PMC ]
[
Abstract ]
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1441-1846
strain :
C57BL/6J .
tissue :
Spinal ganglion .