Gene name:
Arfgef2 (Arfgep2;Big2) ;
Protein name:
Brefeldin A-inhibited guanine nucleotide-exchange protein 2 (Brefeldin A-inhibited GEP 2) ;
Alternative:
ADP-ribosylation factor guanine nucleotide-exchange factor 2 ;
Organism:
Rat (Rattus norvegicus).
General Annotation
Sub Unit:
Homodimer. Interacts with BIG1; both proteins are probably part of the same or very similar macromolecular complexes. Interacts with PRKAR1A, PRKAR2A, PRKAR1B, PRKAR2B, PPP1CC, PDE3A, TNFRSF1A, MYCBP and EXOC7 (By similarity). Interacts with GABRB1, GABRB2 and GABRB3.
Function:
Promotes guanine-nucleotide exchange on ARF1 and ARF3 and to a lower extend on ARF5 and ARF6. Promotes the activation of ARF1/ARF5/ARF6 through replacement of GDP with GTP. Involved in the regulation of Golgi vesicular transport. Required for the integrity of the endosomal compartment. Involved in trafficking from the trans-Golgi network (TGN) to endosomes and is required for membrane association of the AP-1 complex and GGA1. Seems to be involved in recycling of the transferrin receptor from recycling endosomes to the plasma membrane. Probably is involved in the exit of GABA(A) receptors from the endoplasmic reticulum. Involved in constitutive release of tumor necrosis factor receptor 1 via exosome-like vesicles; the function seems to involve PKA and specifically PRKAR2B. Proposed to act as A kinase-anchoring protein (AKAP) and may mediate crosstalk between Arf and PKA pathways.
Subcellular Location:
Cytoplasm
Membrane
Golgi apparatus
Cytoplasm
Perinuclear region
Golgi apparatus
Trans-Golgi network
Endosome
Cytoplasm
Cytoskeleton
Microtubule organizing center
Centrosome
Cell projection
Dendrite
Cytoplasmic vesicle
Cell junction
Synapse
Cytoplasm
Cytoskeleton
Translocates from cytoplasm to membranes upon cAMP treatment. Localized in recycling endosomes.
Protein Attributes:
50:
MQESQTKSMF | VSRALEKILA | DKEVKRPQHS | QLRRACQVAL | DEIKAELEKQ |
100:
RLGAAAPPKA | NFIEADKYFL | PFELACQSKS | PRVVSTSLDC | LQKLIAYGHI |
150:
TGNAPDSGAP | GKRLIDRIVE | TVCNCFQGPQ | TDEGVQLQII | KALLTAVTSP |
200:
HIEIHEGTIL | QTVRTCYNIY | LASKNLINQT | TAKATLTQML | NVIFTRMENQ |
250:
VLQEARELEK | PIQSKPQSPV | IQATAGSPKF | SRLKQSQAQS | KPTTPEKTEL |
300:
PNGDHARSSL | GKVNSENGEA | HRERGSSISG | RAEPSGGSDN | GAQEVVKDIL |
350:
EDVVTSAVKE | AAEKQGLPEP | DQAPGVPECQ | ECTVPPAVDE | NSQTNGIADD |
400:
RQSLSSADNL | EPDAQGHPVA | ARFSHILQKD | AFLVFRSLCK | LSMKPLGEGP |
450:
PDPKSHELRS | KVVSLQLLLS | VLQNAGPVFR | SHEMFVTAIK | QYLCVALSKN |
500:
GVSSVPDVFE | LSLAIFLTLL | SNFKMHLKMQ | IEVFFKEIFL | NILETSTSSF |
550:
EHRWMVIQTL | TRICADAQCV | VDIYVNYDCD | LNAANIFERL | VNDLSKIAQG |
600:
RSGHELGMTP | LQELSLRKKG | LECLVSILKC | MVEWSKDLYV | NPNHQATLGQ |
650:
ERLPDQEMGD | GKGLDMARRC | SVTSVESTVS | SGTQTAIPDD | PEQFEVIKQQ |
700:
KEIIEHGIEL | FNKKPKRGIQ | FLQEQGMLGA | AVEDIAQFLH | QEERLDSTQV |
750:
GEFLGDSTRF | NKEVMYAYVD | QLDFCEKEFV | SALRTFLEGF | RLPGEAQKID |
800:
RLMEKFAARY | IECNQGQTLF | ASADTAYVLA | YSIIMLTTDL | HSPQVKNKMT |
850:
KEQYIKMNRG | INDSKDLPEE | YLSSIYEEIE | GKKIAMKETK | EHTMATKSTK |
900:
QNVASEKQRR | LLYNVEMEQM | AKTAKALMEA | VSHAKAPFTS | ATHLDHVRPM |
950:
FKLVWTPLLA | AYSIGLQNCD | DTEVASLCLE | GIRCAVRIAC | IFGMQLERDA |
1000:
YVQALARFSL | LTASSSITEM | KQKNIDTIKT | LITVAHTDGN | YLGNSWHEIL |
1050:
KCISQLELAQ | LIGTGVKTRY | LSGSGREREG | SLKGHSLAGE | EFMGLGLGNL |
1100:
VSGGVDKRQM | ASFQESVGET | SSQSVVVAVD | RIFTGSTRLD | GNAIVDFVRW |
1150:
LCAVSMDELA | SPHHPRMFSL | QKIVEISYYN | MNRIRLQWSR | IWHVIGDHFN |
1200:
KVGCNPNEDV | AIFAVDSLRQ | LSMKFLEKGE | LANFRFQKDF | LRPFEHIMKK |
1250:
NRSPTIRDMV | IRCIAQMVSS | QAANIRSGWK | NIFAVFHQAA | SDHDGNIVEL |
1300:
AFQTTGHIVS | TIFQHHFPAA | IDSFQDAVKC | LSEFACNAAF | PDTSMEAIRL |
1350:
IRFCGKYVSE | RPRVLQEYTS | DDMNVAPGDR | VWVRGWFPIL | FELSCIINRC |
1400:
KLDVRTRGLT | VMFEIMKSYG | HTFAKHWWQD | LFRIVFRIFD | NMKLPEQQSE |
1450:
KSEWMTTTCN | HALYAICDVF | TQFYEALHEV | LLSDVFAQLQ | WCVKQDNEQL |
1500:
ARSGTNCLEN | LVISNGEKFS | PAVWDETCNC | MLDIFRTTIP | HVLLTWRPAG |
1550:
MEEEVSDRHL | DVDLDRQSLS | SIDRNASERG | QSQLSNPTDD | SWKGAPYANQ |
1600:
KLLASLLIKC | VVQLELIQTI | DNIVFYPATS | KKEDAEHMVA | AQQDTLDADI |
1650:
HIETENQGMY | KFMSSQHLFK | LLDCLQESHS | FSKAFNSNYE | QRTVLWRAGF |
1700:
KGKSKPNLLK | QETSSLACCL | RILFRMYVDE | NRRDSWGEIQ | QRLLTVCSEA |
1750:
LAYFITVNSE | SHREAWTSLL | LLLLTKTLKI | NDEKFKAHAS | VYYPYLCEMM |
1791:
QFDLIPELRA | VLRKFFLRIG | LVYKIWVPEE | PSQVPAASTA | W
Vaild Sequence:
Related Databases
Uniprot:
ELISA Kit
CLIA Kit
Polyclonal Antibody
Monoclonal Antibody
Protein
FOR
Rat
Human
Mouse
ELISA Kit for Rat Brefeldin A-inhibited GEP 2
ELISA Kit for Rat Brefeldin A-inhibited GEP 2
ELISA Kit for Rat Brefeldin A-inhibited GEP 2
CLIA Kit for Rat Brefeldin A-inhibited GEP 2
CLIA Kit for Rat Brefeldin A-inhibited GEP 2
CLIA Kit for Rat Brefeldin A-inhibited GEP 2
Polyclonal Antibody for Rat Brefeldin A-inhibited GEP 2
Polyclonal Antibody for Rat Brefeldin A-inhibited GEP 2
Polyclonal Antibody for Rat Brefeldin A-inhibited GEP 2
Monoclonal Antibody for Rat Brefeldin A-inhibited GEP 2
Monoclonal Antibody for Rat Brefeldin A-inhibited GEP 2
Monoclonal Antibody for Rat Brefeldin A-inhibited GEP 2
Protein for Rat Brefeldin A-inhibited GEP 2
Protein for Rat Brefeldin A-inhibited GEP 2
Protein for Rat Brefeldin A-inhibited GEP 2
R&D Technical Data
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Precision
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Recovery
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Linearity
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References
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[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : NUCLEOTIDE SEQUENCE [MRNA];FUNCTION;SUBCELLULAR LOCATION;INTERACTION WITH GABRB1; GABRB2 AND GABRB3
strain :
Sprague-Dawley .
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Nature428:493-521(2004)
[
PubMed ]
[
Europe PMC ]
[
Abstract ]
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
strain :
Brown Norway .
3.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : SUBCELLULAR LOCATION