Bone morphogenetic protein 4 (Protein name
), BMP4_BOVIN from NCBI database.
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General Annotation
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Antigen Annotation
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3D
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Predicted Eptitope
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Vaild Sequence
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Related Databases
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Gene name:
BMP4;
Protein name:
Bone morphogenetic protein 4(BMP-4);
Alternative:
Organism:
Bovine (Bos taurus).
General Annotation
Sub Unit:
Homodimer; disulfide-linked. Interacts with SOSTDC1, GREM2, RGMA, RGMB and RGMC.
Function:
Induces cartilage and bone formation. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction.
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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.4%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.9%
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Recovery
Recovery was determined by spiking various levels of BMP4 into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
99
96-102
Plasma
98
94-100
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Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BMP4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
96-100%
98-101%
94-99%
96-100%
EDTA plasma(n=5)
95-99%
98-103%
100-103%
102-106%
heparin plasma(n=5)
96-101%
98-100%
95-103%
101-105%
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