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Index > Protein center > Bpi(Gene name) > Rat
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  • Bpi (Gene name),
  • Bactericidal permeability-increasing protein (Protein name ),  BPI_RAT from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Gene name:
    Bpi;
    Protein name:
    Bactericidal permeability-increasing protein(BPI);
    Alternative:

    Organism:
    Rat (Rattus norvegicus). 
    General Annotation
    Sub Unit:
    Monomer. Homodimer; disulfide-linked.
    Function:
    The cytotoxic action of BPI is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope.
    Subcellular Location:
    Secreted Cytoplasmic granule membrane Membrane-associated in polymorphonuclear Leukocytes (PMN) granules.
    Protein Attributes:
    Sequence length:
    482
    Sequence:
    50:
    MAWGPDNVRK | WSSLALLAIV | GTALTAATDP | GFVARISQKG | LDFVCQESMV | 
    100:
    ELQKELLAIS | IPDFSGDFKI | KHLGKGTYEF | YSMAVEGFHI | PDPQIKLLPS | 
    150:
    DGLQLSITSA | SIKISGRWKY | RKNILKASGN | FQLSIQGVSI | IADLILGNDP | 
    200:
    SGRITITCST | CDSHINSVRI | KVSGSMLGWL | IQLFHRKIET | SLKKTIYKKI | 
    250:
    CKIVRNSVSA | KLQPYVKTLP | VVAKVDDITS | IDYSLLAPPM | TTDKFLEGQL | 
    300:
    RGEFFWRGHH | GPFPAVPPVM | NILPNNNYMV | CMGISDYFFN | TAEFAYQESE | 
    350:
    TLKITLRDQL | LAKDARYHLN | TDFLKTFLPE | VAKKFPSMGL | QLLISAPLFA | 
    400:
    HLNIQPSGLS | LSPNLETRAF | VVLPNSSLIP | LFLLGMKTNA | SLEVNAMKNR | 
    450:
    LIGEMKLGRL | LLELKQSNFG | SFKVELLEDV | INYLMSTMVL | PKINEKLRRG | 
    482:
    FPLPLPAGIQ | LINSILYSSQ | NFLLLEADLH | RT
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    String:
    UniGene:
    SMR:
    Pfam:
    Uniprot:
     
    FOR
    ELISA Kit for Rat BPI
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    ELISA Kit for Rat BPI
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    ELISA Kit for Rat BPI
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    ELISA Kit for Rat BPI
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    ELISA Kit for Rat BPI
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    CLIA Kit for Rat BPI
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    U1234r
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    CLIA Kit for Rat BPI
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    U1234h
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    MSDS:
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    96T
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    CLIA Kit for Rat BPI
    MSDS:
    Please sign in first.
    CLIA Kit for Rat BPI
    Cat.:
    U1234b
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    Please sign in first.
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    Packing:
    96T
    CLIA Kit for Rat BPI
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    U1234m
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    Packing:
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    Polyclonal Antibody for Rat BPI
    Polyclonal Antibody for Rat BPI
    Polyclonal Antibody for Rat BPI
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    P1234Rb-r
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat BPI
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    P1234Rb-h
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    40ug/0.2ml
    Polyclonal Antibody for Rat BPI
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    P1234Rb-m
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    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Rat BPI
    Monoclonal Antibody for Rat BPI
    Monoclonal Antibody for Rat BPI
    Monoclonal Antibody for Rat BPI
    Monoclonal Antibody for Rat BPI
    Protein for Rat BPI
    Protein for Rat BPI
    Protein for Rat BPI
    Protein for Rat BPI
    Protein for Rat BPI

    R&D Technical Data
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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

     

    Intra-Assay CV: ≤4.7

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.2

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    Recovery
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    Recovery was determined by spiking various levels of BPI into serum and plasma .

     

    Sample Type

    Average(%)

    Recovery  Range (%)

    Serum

    99

    91-108

    Plasma

    95

    89-97

     

     

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BPI and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    80-92%

    94-105%

    96-103%

    95-108%

    EDTA plasma(n=5)

    91-102%

    96-103%

    89-95%

    86-102%

    heparin plasma(n=5)

    84-98%

     

    85-99%

    79-91%

    96-101%

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    References
    1. 1.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      tissue: Testis.
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