Cbs ELISA Kit for Rat(Rattus norvegicus) | EIAab.comCbs ELISA Kit for Rat(Rattus norvegicus) | EIAab.com

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Cbs (Gene name),

Cystathionine beta-synthase (Protein name ), CBS_RAT from NCBI database.

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General Annotation

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Antigen Annotation

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3D

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Predicted Eptitope

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Vaild Sequence

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Related Databases

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Gene name:

Cbs;

Protein name:

Cystathionine beta-synthase;

Alternative:

Hemoprotein H-450;Beta-thionase;Serine sulfhydrase;

Organism:

Rat (Rattus norvegicus).

General Annotation

Sub Unit:

Homotetramer.

Function:

Only known pyridoxal phosphate-dependent enzyme that contains heme. Important regulator of hydrogen sulfide, especially in the brain, utilizing cysteine instead of serine to catalyze the formation of hydrogen sulfide. Hydrogen sulfide is a gastratransmitter with signaling and cytoprotective effects such as acting as a neuromodulator in the brain to protect neurons against hypoxic injury.

Subcellular Location:

Nucleus Cytoplasm

Protein Attributes:

Sequence length:

561

Sequence:

50:

MPSGTSQCED | GSAGCPQDLE | VQPEKGQLEK | GASGDKERVW | ISPDTPSRCT |

100:

WQLGRPMADS | PHYHTVPTKS | PKILPDILRK | IGNTPMVRIN | RISKNAGLKC |

150:

ELLAKCEFFN | AGGSVKDRIS | LRMIEDAERA | GTLKPGDTII | EPTSGNTGIG |

200:

LALAAAVKGY | RCIIVMPEKM | SMEKVDVLRA | LGAEIVRTPT | NARFDSPESH |

250:

VGVAWRLKNE | IPNSHILDQY | RNASNPLAHY | DDTAEEILQQ | CDGKVDMLVA |

300:

SAGTGGTITG | IARKLKEKCP | GCKIIGVDPE | GSILAEPEEL | NQTEQTAYEV |

350:

EGIGYDFIPT | VLDRAVVDRW | FKSNDDDSFA | FARMLISQEG | LLCGGSSGSA |

400:

MAVAVKAAQE | LKEGQRCVVI | LPDSVRNYMS | KFLSDKWMLQ | KGFMKEELSV |

450:

KRPWWWHLRV | QELSLSAPLT | VLPTVTCEHT | IAILREKGFD | QAPVVNESGA |

500:

ILGMVTLGNM | LSSLLAGKVR | PSDEVCKVLY | KQFKPIHLTD | TLGMLSHILE |

550:

MDHFALVVHE | QIQSRDQAWS | GVVGGPTDRN | NGVSSKQLMV | FGVVTAIDLL |

561:

NFVAAREQTR | K

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

UniGene:

STRING:

10116.ENSRNOP00000039968

SMR:

KEGG:

String:

Pfam:

Uniprot:

FOR

ELISA Kit for Rat Cystathionine beta-synthase

Cat.:

E1957h

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MSDS:

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Packing:

96T

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ELISA Kit for Rat Cystathionine beta-synthase

Cat.:

E1957r

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96T

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ELISA Kit for Rat Cystathionine beta-synthase

Cat.:

E1957m

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96T

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ELISA Kit for Rat Cystathionine beta-synthase

MSDS:

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CLIA Kit for Rat Cystathionine beta-synthase

Cat.:

U1957m

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MSDS:

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Packing:

96T

CLIA Kit for Rat Cystathionine beta-synthase

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CLIA Kit for Rat Cystathionine beta-synthase

Cat.:

U1957h

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96T

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CLIA Kit for Rat Cystathionine beta-synthase

Cat.:

U1957r

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Packing:

96T

Polyclonal Antibody for Rat Cystathionine beta-synthase

Polyclonal Antibody for Rat Cystathionine beta-synthase

Cat.:

P1957Rb-m

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Packing:

40ug/0.2ml

Polyclonal Antibody for Rat Cystathionine beta-synthase

Cat.:

P1957Rb-r

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Packing:

40ug/0.2ml

Polyclonal Antibody for Rat Cystathionine beta-synthase

Cat.:

P1957Rb-h

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Monoclonal Antibody for Rat Cystathionine beta-synthase

Monoclonal Antibody for Rat Cystathionine beta-synthase

Monoclonal Antibody for Rat Cystathionine beta-synthase

Monoclonal Antibody for Rat Cystathionine beta-synthase

Protein for Rat Cystathionine beta-synthase

Protein for Rat Cystathionine beta-synthase

Protein for Rat Cystathionine beta-synthase

Protein for Rat Cystathionine beta-synthase

R&D Technical Data

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s

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Precision

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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.5%

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Recovery

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Recovery was determined by spiking various levels of CBS into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	101	95-103
Plasma	103	97-107

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Linearity

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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CBS and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	95-107%	94-109%	97-104%	99-103%
Saliva(n=3)	93-105%	96-103%	97-104%	95-106%
heparin plasma(n=5)	98-108%	94-110%	99-106%	96-109%

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References

1.

"Rat cystathionine beta-synthase. Gene organization and alternative splicing."
Swaroop M. , Bradley K. , Ohura T. , Tahara T. , Roper M.D. , Rosenberg L.E. , Kraus J.P.
J. Biol. Chem.267:11455-11461(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];PARTIAL PROTEIN SEQUENCE
tissue: Liver.
2.

"Molecular cloning and sequence analysis of cDNA coding for rat liver hemoprotein H-450."
Ishihara S. , Morohashi K. , Sadano H. , Kawabata S. , Gotoh O. , Omura T.
J. Biochem.108:899-902(1990) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];PROTEIN SEQUENCE OF 2-23 AND 39-48;TISSUE SPECIFICITY
strain: Sprague-Dawley.
tissue: Liver.
3.

"The possible role of hydrogen sulfide as an endogenous neuromodulator."
Abe K. , Kimura H.
J. Neurosci.16:1066-1071(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ENZYME ACTIVITY;FUNCTION;TISSUE SPECIFICITY
4.

"Hydrogen sulfide protects neurons against hypoxic injury via stimulation of ATP-sensitive potassium channel/protein kinase C/extracellular signal-regulated kinase/heat shock protein 90 pathway."
Tay A.S. , Hu L.F. , Lu M. , Wong P.T. , Bian J.S.
Neuroscience167:277-286(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ENZYME ACTIVITY;FUNCTION

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