CDC42 ELISA Kit for Human(Homo sapiens) | EIAab.comCDC42 ELISA Kit for Human(Homo sapiens)

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CDC42 (Gene name),

Cell division control protein 42 homolog (Protein name ), CDC42_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

CDC42;

Protein name:

Cell division control protein 42 homolog;

Alternative:

G25K GTP-binding protein;

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

The GTP-bound form interacts with CCPG1 (By similarity). Interacts with CDC42EP1, CDC42EP2, CDC42EP3, CDC42EP4, CDC42EP5, CDC42SE1, CDC42SE2, PARD6A, PARD6B and PARD6G (in a GTP-dependent manner). Interacts with activated CSPG4 and with BAIAP2. Interacts with Zizimin1/DOCK9 and Zizimin2/DOCK11, which activate it by exchanging GDP for GTP. Interacts with NET1 and ARHGAP33/TCGAP. Part of a complex with PARD3, PARD6A or PARD6B and PRKCI or PRKCZ. Interacts with USP6. May interact with ARHGEF16; responsible for the activation of CDC42 by the viral protein HPV16 E6. Interacts with NEK6.

Function:

Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Plays a role in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia.

Subcellular Location:

Cell membrane Lipid-anchor Cytoplasmic side Cytoplasm cytoskeleton centrosome Co-localizes with NEK6 in the centrosome.

Protein Attributes:

Sequence length:

191

Sequence:

50:

100:

150:

191:

AVKYVECSAL | TQKGLKNVFD | EAILAALEPP | EPKKSRRCVL | L

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

SMR:

P60953

MIM:

116952

Pfam:

PF00071

KEGG:

hsa:998

STRING:

9606.ENSP00000314458

UniGene:

Hs.467637

String:

P60953

Uniprot:

P60953

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ELISA Kit for Human Cell division control protein 42 homolog

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E0321h

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ELISA Kit for Human Cell division control protein 42 homolog

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E0321b

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ELISA Kit for Human Cell division control protein 42 homolog

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E0321m

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ELISA Kit for Human Cell division control protein 42 homolog

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E0321p

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ELISA Kit for Human Cell division control protein 42 homolog

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E0321d

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ELISA Kit for Human Cell division control protein 42 homolog

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E0321r

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ELISA Kit for Human Cell division control protein 42 homolog

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E0321c

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96T

CLIA Kit for Human Cell division control protein 42 homolog

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U0321m

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96T

CLIA Kit for Human Cell division control protein 42 homolog

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U0321d

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96T

CLIA Kit for Human Cell division control protein 42 homolog

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U0321r

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96T

CLIA Kit for Human Cell division control protein 42 homolog

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U0321b

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96T

CLIA Kit for Human Cell division control protein 42 homolog

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U0321p

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96T

CLIA Kit for Human Cell division control protein 42 homolog

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U0321c

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96T

CLIA Kit for Human Cell division control protein 42 homolog

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U0321h

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Polyclonal Antibody for Human Cell division control protein 42 homolog

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P0321Rb-h

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Polyclonal Antibody for Human Cell division control protein 42 homolog

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P0321Rb-r

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Polyclonal Antibody for Human Cell division control protein 42 homolog

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P0321Rb-m

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Monoclonal Antibody for Human Cell division control protein 42 homolog

Protein for Human Cell division control protein 42 homolog

R&D Technical Data

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Precision

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Recovery

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Linearity

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References

1.

"Modification of mineralocorticoid receptor function by Rac1 GTPase: implication in proteinuric kidney disease."
Shibata S. , Nagase M. , Yoshida S. , Kawarazaki W. , Kurihara H. , Tanaka H. , Miyoshi J. , Takai Y. , Fujita T.
Nat. Med.14:1370-1376(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: MUTAGENESIS OF GLY-12 AND THR-17
2.

"Molecular cloning and expression of a G25K cDNA, the human homolog of the yeast cell cycle gene CDC42."
Munemitsu S. , Innis M.A. , Clark R. , McCormick F. , Ullrich A. , Polakis P.
Mol. Cell. Biol.10:5977-5982(1990) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
tissue: Fetal brain.
3.

"Molecular cloning of the gene for the human placental GTP-binding protein Gp (G25K): identification of this GTP-binding protein as the human homolog of the yeast cell-division-cycle protein CDC42."
Shinjo K. , Koland J.G. , Hart M.J. , Narasimhan V. , Johnson D.I. , Evans T. , Cerione R.A.
Proc. Natl. Acad. Sci. U.S.A.87:9853-9857(1990) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2)
tissue: Placenta.
4.

Rhodes S. , Huckle E.
Submitted (1999-10) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2)
5.

"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
Puhl H.L. III , Ikeda S.R. , Aronstam R.S.
Submitted (2002-04) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2)
tissue: Brain.
tissue: Placenta.
6.

NIEHS SNPs program
Submitted (2004-06) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
7.

"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G. , Barlow K.F. , McLay K.E. , Kaul R. , Swarbreck D. , Dunham A. , Scott C.E. , Howe K.L. , Woodfine K. , Spencer C.C.A. , Jones M.C. , Gillson C. , Searle S. , Zhou Y. , Kokocinski F. , McDonald L. , Evans R. , Phillips K. , more...
Nature441:315-321(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
8.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2)
tissue: Cervix.
tissue: Placenta.
tissue: Uterus.
9.

Lubec G. , Vishwanath V. , Chen W.-Q. , Sun Y.
Submitted (2008-12) to the UniProtKB

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 67-83 (ISOFORM 2);PARTIAL PROTEIN SEQUENCE (ISOFORM 1);IDENTIFICATION BY MASS SPECTROMETRY
tissue: Brain.
tissue: Cajal-Retzius cell.
tissue: Fetal brain cortex.
10.

"Regulation of the human neutrophil NADPH oxidase by rho-related G-proteins."
Kwong C.H. , Malech H.L. , Rotrosen D. , Leto T.L.
Biochemistry32:5711-5717(1993) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 97-107; 134-144 AND 167-183 (ISOFORM 2)
tissue: Neutrophil.
11.

"Characterization of G25K, a GTP-binding protein containing a novel putative nucleotide binding domain."
Polakis P.G. , Snyderman R. , Evans T.
Biochem. Biophys. Res. Commun.160:25-32(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PARTIAL PROTEIN SEQUENCE
12.

"The Borgs, a new family of Cdc42 and TC10 GTPase-interacting proteins."
Joberty G. , Perlungher R.R. , Macara I.G.
Mol. Cell. Biol.19:6585-6597(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH CDC42EP1; CDC42EP2; CDC42EP3 AND CDC42EP5
tissue: Embryo.
13.

"Melanoma chondroitin sulphate proteoglycan regulates cell spreading through Cdc42, Ack-1 and p130cas."
Eisenmann K.M. , McCarthy J.B. , Simpson M.A. , Keely P.J. , Guan J.-L. , Tachibana K. , Lim L. , Manser E. , Furcht L.T. , Iida J.
Nat. Cell Biol.1:507-513(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH CSPG4
14.

"SPECs, small binding proteins for Cdc42."
Pirone D.M. , Fukuhara S. , Gutkind J.S. , Burbelo P.D.
J. Biol. Chem.275:22650-22656(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH CDC42SE1 AND CDC42SE2
15.

"The mammalian homologue of the Caenorhabditis elegans polarity protein PAR-6 is a binding partner for the Rho GTPases Cdc42 and Rac1."
Johansson A.-S. , Driessens M. , Aspenstroem P.
J. Cell Sci.113:3267-3275(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH PARD6A;MUTAGENESIS OF GLY-12
16.

"IRSp53 is an essential intermediate between Rac and WAVE in the regulation of membrane ruffling."
Miki H. , Yamaguchi H. , Suetsugu S. , Takenawa T.
Nature408:732-735(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH BAIAP2
17.

"Human homologues of the Caenorhabditis elegans cell polarity protein PAR6 as an adaptor that links the small GTPases Rac and Cdc42 to atypical protein kinase C."
Noda Y. , Takeya R. , Ohno S. , Naito S. , Ito T. , Sumimoto H.
Genes Cells6:107-119(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH PARD6A; PARD6B AND PARD6G;SUBUNIT OF A COMPLEX CONTAINING PRKCI AND PARD6B;MUTAGENESIS OF THR-17 AND GLN-61
18.

"The integrin cytoplasmic domain-associated protein ICAP-1 binds and regulates Rho family GTPases during cell spreading."
Degani S. , Balzac F. , Brancaccio M. , Guazzone S. , Retta S.F. , Silengo L. , Eva A. , Tarone G.
J. Cell Biol.156:377-387(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH ITGB1BP1
19.

"Zizimin1, a novel Cdc42 activator, reveals a new GEF domain for Rho proteins."
Meller N. , Irani-Tehrani M. , Kiosses W.B. , Del Pozo M.A. , Schwartz M.A.
Nat. Cell Biol.4:639-647(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH DOCK9;ACTIVATION BY DOCK9
20.

"Epidermal growth factor-dependent regulation of Cdc42 is mediated by the Src tyrosine kinase."
Tu S. , Wu W.J. , Wang J. , Cerione R.A.
J. Biol. Chem.278:49293-49300(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT TYR-64 BY SRC
21.

"The TRE17 oncogene encodes a component of a novel effector pathway for Rho GTPases Cdc42 and Rac1 and stimulates actin remodeling."
Masuda-Robens J.M. , Kutney S.N. , Qi H. , Chou M.M.
Mol. Cell. Biol.23:2151-2161(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH USP6
22.

"Regulation of dendritic branching and filopodia formation in hippocampal neurons by specific acylated protein motifs."
Gauthier-Campbell C. , Bredt D.S. , Murphy T.H. , El-Husseini A.
Mol. Biol. Cell15:2205-2217(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;MUTAGENESIS OF GLY-12 AND THR-17
23.

"Ect2 and MgcRacGAP regulate the activation and function of Cdc42 in mitosis."
Oceguera-Yanez F. , Kimura K. , Yasuda S. , Higashida C. , Kitamura T. , Hiraoka Y. , Haraguchi T. , Narumiya S.
J. Cell Biol.168:221-232(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;SUBCELLULAR LOCATION
24.

"Ack1 mediates Cdc42-dependent cell migration and signaling to p130Cas."
Modzelewska K. , Newman L.P. , Desai R. , Keely P.J.
J. Biol. Chem.281:37527-37535(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN CELL MIGRATION;INTERACTION WITH BCAR1; TNK2 AND CRK
25.

"The fic domain: regulation of cell signaling by adenylylation."
Worby C.A. , Mattoo S. , Kruger R.P. , Corbeil L.B. , Koller A. , Mendez J.C. , Zekarias B. , Lazar C. , Dixon J.E.
Mol. Cell34:93-103(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: AMPYLATION AT TYR-32;MUTAGENESIS OF TYR-32
26.

"AMPylation of Rho GTPases by Vibrio VopS disrupts effector binding and downstream signaling."
Yarbrough M.L. , Li Y. , Kinch L.N. , Grishin N.V. , Ball H.L. , Orth K.
Science323:269-272(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: AMPYLATION AT THR-35
27.

"Characterization of hNek6 interactome reveals an important role for its short N-terminal domain and colocalization with proteins at the centrosome."
Vaz Meirelles G. , Ferreira Lanza D.C. , da Silva J.C. , Santana Bernachi J. , Paes Leme A.F. , Kobarg J.
J. Proteome Res.9:6298-6316(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION;INTERACTION WITH NEK6
28.

"Initial characterization of the human central proteome."
Burkard T.R. , Planyavsky M. , Kaupe I. , Breitwieser F.P. , Buerckstuemmer T. , Bennett K.L. , Superti-Furga G. , Colinge J.
BMC Syst. Biol.5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
29.

"The HPV16 E6 binding protein Tip-1 interacts with ARHGEF16, which activates Cdc42."
Oliver A.W. , He X. , Borthwick K. , Donne A.J. , Hampson L. , Hampson I.N.
Br. J. Cancer104:324-331(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH ARHGEF16
30.

"ARHGDIA: a novel gene implicated in nephrotic syndrome."
Gupta I.R. , Baldwin C. , Auguste D. , Ha K.C. , El Andalousi J. , Fahiminiya S. , Bitzan M. , Bernard C. , Akbari M.R. , Narod S.A. , Rosenblatt D.S. , Majewski J. , Takano T.
J. Med. Genet.50:330-338(2013) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH ARHGDIA
31.

"Definition of the switch surface in the solution structure of Cdc42Hs."
Feltham J.L. , Dotsch V. , Raza S. , Manor D. , Cerione R.A. , Sutcliffe M.J. , Wagner G. , Oswald R.E.
Biochemistry36:8755-8766(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR
32.

"Identification of the binding surface on Cdc42Hs for p21-activated kinase."
Guo W. , Sutcliffe M.J. , Cerione R.A. , Oswald R.E.
Biochemistry37:14030-14037(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR
33.

"Crystal structure of a small G protein in complex with the GTPase-activating protein rhoGAP."
Rittinger K. , Walker P.A. , Eccleston J.F. , Nurmahomed K. , Owen D. , Laue E. , Gamblin S.J. , Smerdon S.J.
Nature388:693-697(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF COMPLEX WITH RHOGAP
34.

"Nucleotide binding to the G12V-mutant of Cdc42 investigated by X-ray diffraction and fluorescence spectroscopy: two different nucleotide states in one crystal."
Rudolph M.G. , Wittinghofer A. , Vetter I.R.
Protein Sci.8:778-787(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF VAL-12 MUTANT
35.

"The structure determination of CDC42Hs and GDP complex."
Kongsaeree P. , Cerione R.A. , Clardy J.C.
Submitted (1997-06) to the PDB data bank

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS)
36.

"Activation of Rho GTPases by DOCK exchange factors is mediated by a nucleotide sensor."
Yang J. , Zhang Z. , Roe S.M. , Marshall C.J. , Barford D.
Science325:1398-1402(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 1-188 IN COMPLEX WITH DOCK9
37.

"Structural basis of Fic-mediated adenylylation."
Xiao J. , Worby C.A. , Mattoo S. , Sankaran B. , Dixon J.E.
Nat. Struct. Mol. Biol.17:1004-1010(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 1-181 IN COMPLEX WITH H.SOMNUS IBPA AND GDP;AMPYLATION AT TYR-32

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