Ugcg ELISA Kit for Rat(Rattus norvegicus) | EIAab.comUgcg ELISA Kit for Rat(Rattus norvegicus) | EIAab.com

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Ugcg (Gene name),

Ceramide glucosyltransferase (Protein name ), CEGT_RAT from NCBI database.

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General Annotation

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Antigen Annotation

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3D

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Predicted Eptitope

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Vaild Sequence

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Gene name:

Ugcg;

Protein name:

Ceramide glucosyltransferase;

Alternative:

Glucosylceramide synthase(GCS);GLCT-1;UDP-glucose:N-acylsphingosine D-glucosyltransferase;UDP-glucose ceramide glucosyltransferase;

Organism:

Rat (Rattus norvegicus).

General Annotation

Sub Unit:

N/A

Function:

Catalyzes the first glycosylation step in glycosphingolipid biosynthesis, the transfer of glucose to ceramide. Able to use UDP-galactose to synthesize galactosylceramide with 10% of efficiency with which it utilizes UDP-glucose. May also serve as a "flippase".

Subcellular Location:

Golgi apparatus membrane Multi-pass membrane protein

Protein Attributes:

Sequence length:

394

Sequence:

50:

MALLDLAQEG | MALFGFVLFV | VLWLMHFMSI | IYTRLHLNKK | ATDKQPYSKL |

100:

PGVSLLKPLK | GVDPNLINNL | ETFFELDYPK | YEVLLCVQDH | DDPAIEVCKK |

150:

LLGKYPNVDA | RLFIGGKKVG | INPKINNLMP | AYEVAKYDLI | WICDSGIRVI |

200:

PDTLTDMVNQ | MTERVGLVHG | LPYVADRQGF | AATLEQVYFG | TSHPRSYISA |

250:

NVTGFKCVTG | MSCLMRKDVL | DQAGGLIAFA | QYIAEDYFMA | KAIADRGWKF |

300:

SMSTQVAMQN | SGSYSISQFQ | SRMIRWTKLR | INMLPATIIC | EPISECFVAS |

350:

LIIGWAAHHV | FRWDIMVFFM | CHCLAWFIFD | YIQLRGVQGG | TLCFSKLDYA |

394:

VAWFIRESMT | IYIFLSALWD | PTISWRAGRY | RLRCGGTAEE | ILDV

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

Pfam:

String:

UniGene:

Pfam:

KEGG:

Uniprot:

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ELISA Kit for Rat Ceramide glucosyltransferase

Cat.:

E1742h

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Packing:

96T

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ELISA Kit for Rat Ceramide glucosyltransferase

Cat.:

E1742m

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96T

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ELISA Kit for Rat Ceramide glucosyltransferase

Cat.:

E1742r

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Packing:

96T

CLIA Kit for Rat Ceramide glucosyltransferase

Cat.:

U1742r

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Packing:

96T

CLIA Kit for Rat Ceramide glucosyltransferase

Cat.:

U1742h

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96T

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CLIA Kit for Rat Ceramide glucosyltransferase

Cat.:

U1742m

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MSDS:

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Packing:

96T

Polyclonal Antibody for Rat Ceramide glucosyltransferase

Cat.:

P1742Rb-m

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Packing:

40ug/0.2ml

Polyclonal Antibody for Rat Ceramide glucosyltransferase

Cat.:

P1742Rb-h

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Packing:

40ug/0.2ml

Polyclonal Antibody for Rat Ceramide glucosyltransferase

Cat.:

P1742Rb-r

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Packing:

40ug/0.2ml

Monoclonal Antibody for Rat Ceramide glucosyltransferase

Monoclonal Antibody for Rat Ceramide glucosyltransferase

Monoclonal Antibody for Rat Ceramide glucosyltransferase

Protein for Rat Ceramide glucosyltransferase

Protein for Rat Ceramide glucosyltransferase

Protein for Rat Ceramide glucosyltransferase

R&D Technical Data

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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤7.4%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤12.1%

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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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Recovery

Recovery was determined by spiking various levels of CEGT into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	95-104
Plasma	98	92-100

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Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CEGT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	97-103%	95-103%	94-104%	93-104%
EDTA plasma(n=5)	92-104%	96-101%	90-105%	93-104%
heparin plasma(n=5)	95-100%	94-102%	92-103%	95-101%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Histidine-193 of rat glucosylceramide synthase resides in a UDP-glucose- and inhibitor (D-threo-1-phenyl-2-decanoylamino-3-morpholinopropan-1-ol)-binding region: a biochemical and mutational study."
Wu K. , Marks D.L. , Watanabe R. , Paul P. , Rajan N. , Pagano R.E.
Biochem. J.341:395-400(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];FUNCTION;CATALYTIC ACTIVITY;MUTAGENESIS OF HIS-26; HIS-36; HIS-90; HIS-169; HIS-193; HIS-308; HIS-309; 308-HIS-HIS-309 AND HIS-322
strain: Sprague-Dawley.
tissue: Brain.
2.

"Purification and characterization of glucosylceramide synthase."
Buenning C. , Orci L. , Hirabayashi Y. , Wieland F.T. , Jeckel D.
Submitted (1998-02) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
strain: Wistar.
tissue: Liver.
3.

"Oligomerization and topology of the Golgi membrane protein glucosylceramide synthase."
Marks D.L. , Wu K. , Paul P. , Kamisaka Y. , Watanabe R. , Pagano R.E.
J. Biol. Chem.274:451-456(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION;MEMBRANE TOPOLOGY

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