Gene name:
CDK5RAP2 (CEP215;KIAA1633) ;
Protein name:
CDK5 regulatory subunit-associated protein 2 ;
Alternative:
Centrosome-associated protein 215 ;CDK5 activator-binding protein C48 ;
Organism:
Human (Homo sapiens).
General Annotation
Sub Unit:
Interacts with CDK5R1 (p35 form). CDK5RAP1, CDK5RAP2 and CDK5RAP3 show competitive binding to CDK5R1. Probably forms a complex with CDK5R1 and CDK5 (By similarity). Interacts with PCNT; the interaction is leading to centrosomal and Golgi localization of CDK5RAP2 and PCNT. Interacts with AKAP9; the interaction is leading to Golgi localization of CDK5RAP2 and AKAP9. Interacts with MAPRE1; the interaction is leading to microtubule attachment at plus ends of CDK5RAP2 and MAPRE1. Interacts with TUBG1; the interaction is leading to the centrosomal localization of CDK5RAP2 and TUBG1. Interacts with TUBGCP3. Interacts with CALM1. Interacts with CDC20.
Function:
Potential regulator of CDK5 activity via its interaction with CDK5R1. Negative regulator of centriole disengagement (licensing) which maintains centriole engagement and cohesion. Involved in regulation of mitotic spindle orientation (By similarity). Plays a role in the spindle checkpoint activation by acting as a transcriptional regulator of both BUBR1 and MAD2 promoter. Together with MAPRE1, it may promote microtubule polymerization, bundle formation, growth and dynamics at the plus ends.
Subcellular Location:
Cytoplasm
cytoskeleton
centrosome
Golgi apparatus
Cytoplasm
Found in the pericentriolar region adhering to the surface of the centrosome and in the region of the centrosomal appendages. Localizes to microtubule plus ends.
Protein Attributes:
50:
MMDLVLEEDV | TVPGTLSGCS | GLVPSVPDDL | DGINPNAGLG | NGLLPNVSEE |
100:
TVSPTRARNM | KDFENQITEL | KKENFNLKLR | IYFLEERMQQ | EFHGPTEHIY |
150:
KTNIELKVEV | ESLKRELQER | EQLLIKASKA | VESLAEAGGS | EIQRVKEDAR |
200:
KKVQQVEDLL | TKRILLLEKD | VTAAQAELEK | AFAGTETEKA | LRLRLESKLS |
250:
EMKKMHEGDL | AMALVLDEKD | RLIEELKLSL | KSKEALIQCL | KEEKSQMACP |
300:
DENVSSGELR | GLCAAPREEK | ERETEAAQME | HQKERNSFEE | RIQALEEDLR |
350:
EKEREIATEK | KNSLKRDKAI | QGLTMALKSK | EKKVEELNSE | IEKLSAAFAK |
400:
AREALQKAQT | QEFQGSEDYE | TALSGKEALS | AALRSQNLTK | STENHRLRRS |
450:
IKKITQELSD | LQQERERLEK | DLEEAHREKS | KGDCTIRDLR | NEVEKLRNEV |
500:
NEREKAMENR | YKSLLSESNK | KLHNQEQVIK | HLTESTNQKD | VLLQKFNEKD |
550:
LEVIQQNCYL | MAAEDLELRS | EGLITEKCSS | QQPPGSKTIF | SKEKKQSSDY |
600:
EELIQVLKKE | QDIYTHLVKS | LQESDSINNL | QAELNKIFAL | RKQLEQDVLS |
650:
YQNLRKTLEE | QISEIRRREE | ESFSLYSDQT | SYLSICLEEN | NRFQVEHFSQ |
700:
EELKKKVSDL | IQLVKELYTD | NQHLKKTIFD | LSCMGFQGNG | FPDRLASTEQ |
750:
TELLASKEDE | DTIKIGEDDE | INFLSDQHLQ | QSNEIMKDLS | KGGCKNGYLR |
800:
HTESKISDCD | GAHAPGCLEE | GAFINLLAPL | FNEKATLLLE | SRPDLLKVVR |
850:
ELLLGQLFLT | EQEVSGEHLD | GKTEKTPKQK | GELVHFVQTN | SFSKPHDELK |
900:
LSCEAQLVKA | GEVPKVGLKD | ASVQTVATEG | DLLRFKHEAT | REAWEEKPIN |
950:
TALSAEHRPE | NLHGVPGWQA | ALLSLPGITN | REAKKSRLPI | LIKPSRSLGN |
1000:
MYRLPATQEV | VTQLQSQILE | LQGELKEFKT | CNKQLHQKLI | LAEAVMEGRP |
1050:
TPDKTLLNAQ | PPVGAAYQDS | PGEQKGIKTT | SSVWRDKEMD | SDQQRSYEID |
1100:
SEICPPDDLA | SLPSCKENPE | DVLSPTSVAT | YLSSKSQPSA | KVSVMGTDQS |
1150:
ESINTSNETE | YLKQKIHDLE | TELEGYQNFI | FQLQKHSQCS | EAIITVLCGT |
1200:
EGAQDGLSKP | KNGSDGEEMT | FSSLHQVRYV | KHVKILGPLA | PEMIDSRVLE |
1250:
NLKQQLEEQE | YKLQKEQNLN | MQLFSEIHNL | QNKFRDLSPP | RYDSLVQSQA |
1300:
RELSLQRQQI | KDGHGICVIS | RQHMNTMIKA | FEELLQASDV | DYCVAEGFQE |
1350:
QLNQCAELLE | KLEKLFLNGK | SVGVEMNTQN | ELMERIEEDN | LTYQHLLPES |
1400:
PEPSASHALS | DYETSEKSFF | SRDQKQDNET | EKTSVMVNSF | SQDLLMEHIQ |
1450:
EIRTLRKRLE | ESIKTNEKLR | KQLERQGSEF | VQGSTSIFAS | GSELHSSLTS |
1500:
EIHFLRKQNQ | ALNAMLIKGS | RDKQKENDKL | RESLSRKTVS | LEHLQREYAS |
1550:
VKEENERLQK | EGSEKERHNQ | QLIQEVRCSG | QELSRVQEEV | KLRQQLLSQN |
1600:
DKLLQSLRVE | LKAYEKLDEE | HRRLREASGE | GWKGQDPFRD | LHSLLMEIQA |
1650:
LRLQLERSIE | TSSTLQSRLK | EQLARGAEKA | QEGALTLAVQ | AVSIPEVPLQ |
1700:
PDKHDGDKYP | MESDNSFDLF | DSSQAVTPKS | VSETPPLSGN | DTDSLSCDSG |
1750:
SSATSTPCVS | RLVTGHHLWA | SKNGRHVLGL | IEDYEALLKQ | ISQGQRLLAE |
1800:
MDIQTQEAPS | STSQELGTKG | PHPAPLSKFV | SSVSTAKLTL | EEAYRRLKLL |
1850:
WRVSLPEDGQ | CPLHCEQIGE | MKAEVTKLHK | KLFEQEKKLQ | NTMKLLQLSK |
1893:
RQEKVIFDQL | VVTHKILRKA | RGNLELRPGG | AHPGTCSPSR | PGS
Vaild Sequence:
Related Databases
Uniprot:
ELISA Kit
CLIA Kit
Polyclonal Antibody
Monoclonal Antibody
Protein
FOR
Human
Mouse
Rat
ELISA Kit for Human CDK5 regulatory subunit-associated protein 2
ELISA Kit for Human CDK5 regulatory subunit-associated protein 2
ELISA Kit for Human CDK5 regulatory subunit-associated protein 2
CLIA Kit for Human CDK5 regulatory subunit-associated protein 2
CLIA Kit for Human CDK5 regulatory subunit-associated protein 2
CLIA Kit for Human CDK5 regulatory subunit-associated protein 2
Polyclonal Antibody for Human CDK5 regulatory subunit-associated protein 2
Polyclonal Antibody for Human CDK5 regulatory subunit-associated protein 2
Polyclonal Antibody for Human CDK5 regulatory subunit-associated protein 2
Monoclonal Antibody for Human CDK5 regulatory subunit-associated protein 2
Monoclonal Antibody for Human CDK5 regulatory subunit-associated protein 2
Monoclonal Antibody for Human CDK5 regulatory subunit-associated protein 2
Protein for Human CDK5 regulatory subunit-associated protein 2
Protein for Human CDK5 regulatory subunit-associated protein 2
Protein for Human CDK5 regulatory subunit-associated protein 2
R&D Technical Data
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Precision
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Recovery
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Linearity
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[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4);VARIANT GLN-289
tissue :
Brain .
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[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : SEQUENCE REVISION
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[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
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Abstract ]
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4);NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 575-1893 (ISOFORM 2);VARIANTS GLN-289 AND LEU-1540
tissue :
Amygdala .
tissue :
Retina .
tissue :
Testis .
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[
Abstract ]
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
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[
Abstract ]
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 950-1893 (ISOFORM 3);NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1576-1654 (ISOFORM 4);VARIANT LEU-1540
tissue :
Kidney .
tissue :
Teratocarcinoma .
7.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1401-1893
tissue :
Lung .
8.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : TISSUE SPECIFICITY
9.
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]
tissue :
Lymphoblast .
10.
"A centrosomal mechanism involving CDK5RAP2 and CENPJ controls brain size."
Bond J.
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Roberts E.
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Hampshire D.J.
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Silva E.O.
,
Costa S.M.R.
,
Baralle D.
,
Raponi M.
,
Karbani G.
,
Rashid Y.
,
Jafri H.
,
Bennett C.
,
Corry P.
,
Walsh C.A.
,
Woods C.G.
more...
Nat. Genet.37:353-355(2005)
[
PubMed ]
[
Europe PMC ]
[
Abstract ]
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Cited for : INVOLVEMENT IN MCPH3
11.
Bond J.
,
Roberts E.
,
Springell K.
,
Lizarraga S.B.
,
Scott S.
,
Higgins J.
,
Hampshire D.J.
,
Morrison E.E.
,
Leal G.F.
,
Silva E.O.
,
Costa S.M.R.
,
Baralle D.
,
Raponi M.
,
Karbani G.
,
Rashid Y.
,
Jafri H.
,
Bennett C.
,
Corry P.
,
Walsh C.A.
,
Woods C.G.
more...
Nat. Genet.37:555-555(2005)
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Cited for : ERRATUM
12.
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Cited for : FUNCTION;SUBCELLULAR LOCATION
13.
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Cited for : FUNCTION;SUBCELLULAR LOCATION;INTERACTION WITH TUBG1 AND TUBGCP3
14.
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Cited for : PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1238 AND SER-1893;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue :
Cervix carcinoma .
15.
"CDK5RAP2 is required for spindle checkpoint function."
Zhang X.
,
Liu D.
,
Lv S.
,
Wang H.
,
Zhong X.
,
Liu B.
,
Wang B.
,
Liao J.
,
Li J.
,
Pfeifer G.P.
,
Xu X.
Cell Cycle8:1206-1216(2009)
[
PubMed ]
[
Europe PMC ]
[
Abstract ]
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Cited for : FUNCTION;SUBCELLULAR LOCATION;INTERACTION WITH CDC20
16.
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Cited for : FUNCTION;INTERACTION WITH MAPRE1;SUBCELLULAR LOCATION;MUTAGENESIS OF LEU-938 AND PRO-939
17.
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Cited for : SUBCELLULAR LOCATION
18.
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Cited for : PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1238;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue :
Leukemic T-cell .
19.
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Cited for : SUBCELLULAR LOCATION;INTERACTION WITH AKAP9; CALM1 AND PCNT;MUTAGENESIS OF LYS-1865 AND LYS-1869
20.
"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V.
,
Vermeulen M.
,
Santamaria A.
,
Kumar C.
,
Miller M.L.
,
Jensen L.J.
,
Gnad F.
,
Cox J.
,
Jensen T.S.
,
Nigg E.A.
,
Brunak S.
,
Mann M.
Sci. Signal.3:RA3-RA3(2010)
[
PubMed ]
[
Europe PMC ]
[
Abstract ]
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Cited for : PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-547 AND THR-1001;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue :
Cervix carcinoma .
21.
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Cited for : IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]