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Index > Protein center > ALB(Gene name) > Bovine
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  • ALB (Gene name),
  • Serum albumin (Protein name ),  ALBU_BOVIN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • 3D
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Gene name:
    ALB;
    Protein name:
    Serum albumin;
    Alternative:
    BSA;
    Organism:
    Bovine (Bos taurus). 
    General Annotation
    Sub Unit:
    N/A
    Function:
    Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
    Subcellular Location:
    Secreted
    Protein Attributes:
    Sequence length:
    607
    Sequence:
    50:
    MKWVTFISLL | LLFSSAYSRG | VFRRDTHKSE | IAHRFKDLGE | EHFKGLVLIA | 
    100:
    FSQYLQQCPF | DEHVKLVNEL | TEFAKTCVAD | ESHAGCEKSL | HTLFGDELCK | 
    150:
    VASLRETYGD | MADCCEKQEP | ERNECFLSHK | DDSPDLPKLK | PDPNTLCDEF | 
    200:
    KADEKKFWGK | YLYEIARRHP | YFYAPELLYY | ANKYNGVFQE | CCQAEDKGAC | 
    250:
    LLPKIETMRE | KVLASSARQR | LRCASIQKFG | ERALKAWSVA | RLSQKFPKAE | 
    300:
    FVEVTKLVTD | LTKVHKECCH | GDLLECADDR | ADLAKYICDN | QDTISSKLKE | 
    350:
    CCDKPLLEKS | HCIAEVEKDA | IPENLPPLTA | DFAEDKDVCK | NYQEAKDAFL | 
    400:
    GSFLYEYSRR | HPEYAVSVLL | RLAKEYEATL | EECCAKDDPH | ACYSTVFDKL | 
    450:
    KHLVDEPQNL | IKQNCDQFEK | LGEYGFQNAL | IVRYTRKVPQ | VSTPTLVEVS | 
    500:
    RSLGKVGTRC | CTKPESERMP | CTEDYLSLIL | NRLCVLHEKT | PVSEKVTKCC | 
    550:
    TESLVNRRPC | FSALTPDETY | VPKAFDEKLF | TFHADICTLP | DTEKQIKKQT | 
    600:
    ALVELLKHKP | KATEEQLKTV | MENFVAFVDK | CCAADDKEAC | FAVEGPKLVV | 
    607:
    STQTALA
    3D Structure:
    N/A
    Predicted Eptitope:
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    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    SMR:
    String:
    UniGene:
    UniGene:
    Pfam:
    Uniprot:
     
    FOR
    ELISA Kit for Bovine Serum albumin
    MSDS:
    Please sign in first.
    ELISA Kit for Bovine Serum albumin
    Cat.:
    E1028h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Bovine Serum albumin
    Cat.:
    E1028r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Bovine Serum albumin
    Cat.:
    E1028d
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    ELISA Kit for Bovine Serum albumin
    Cat.:
    E1028c
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Bovine Serum albumin
    Cat.:
    E1028p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Bovine Serum albumin
    Cat.:
    E1028m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Bovine Serum albumin
    MSDS:
    Please sign in first.
    CLIA Kit for Bovine Serum albumin
    MSDS:
    Please sign in first.
    CLIA Kit for Bovine Serum albumin
    Cat.:
    U1028m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Bovine Serum albumin
    Cat.:
    U1028r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Bovine Serum albumin
    MSDS:
    Please sign in first.
    CLIA Kit for Bovine Serum albumin
    Cat.:
    U1028h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Bovine Serum albumin
    Cat.:
    U1028d
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Bovine Serum albumin
    Cat.:
    U1028c
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Bovine Serum albumin
    Cat.:
    U1028p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Bovine Serum albumin
    Polyclonal Antibody for Bovine Serum albumin
    Polyclonal Antibody for Bovine Serum albumin
    Polyclonal Antibody for Bovine Serum albumin
    Cat.:
    P1028Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Bovine Serum albumin
    Polyclonal Antibody for Bovine Serum albumin
    Cat.:
    P1028Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Bovine Serum albumin
    Polyclonal Antibody for Bovine Serum albumin
    Cat.:
    P1028Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Bovine Serum albumin
    Monoclonal Antibody for Bovine Serum albumin
    Cat.:
    M1028m
    Price:
    Please sign in first.
    Packing:
    40ug
    Monoclonal Antibody for Bovine Serum albumin
    Monoclonal Antibody for Bovine Serum albumin
    Monoclonal Antibody for Bovine Serum albumin
    Monoclonal Antibody for Bovine Serum albumin
    Monoclonal Antibody for Bovine Serum albumin
    Monoclonal Antibody for Bovine Serum albumin
    Protein for Bovine Serum albumin
    Protein for Bovine Serum albumin
    Protein for Bovine Serum albumin
    Protein for Bovine Serum albumin
    Protein for Bovine Serum albumin
    Protein for Bovine Serum albumin
    Protein for Bovine Serum albumin
    Protein for Bovine Serum albumin

    R&D Technical Data
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    s

    s

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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤8.2%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤10.7%

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

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    Recovery
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    Recovery was determined by spiking various levels of ALBU into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    100

    95-105

    Plasma

    98

    93-104

     

     

     

     

    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ALBU and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    96-104%

    93-106%

    94-105%

    95-106%

    EDTA plasma(n=5)

    92-107%

    96-105%

    93-107%

    96-103%

    heparin plasma(n=5)

    95-106%

     

    97-103%

    97-103%

    94-105%

     

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    EDTA plasma(n=5)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

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    References
    1. 1.
      "Bovine serum albumin: cDNA sequence and expression."
      Holowachuk E.W. , Stoltenborg J.K. , Reed R.G. , Peters T. Jr.
      Submitted (1991-08) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
    2. 2.
      "The bovine serum albumin mRNA."
      Barry T. , Power S. , Gannon F.
      Submitted (1994-07) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];VARIANT THR-214
      tissue: Liver.
    3. 3.
      "Differential binding of IgG and IgA antibodies to antigenic determinants of bovine serum albumin."
      Hilger C. , Grigioni F. , De Beaufort C. , Michel G. , Freilinger J. , Hentges F.
      Clin. Exp. Immunol.123:387-394(2001) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
      tissue: Liver.
    4. 4.
      "The complete cDNA sequence of bovine serum albumin."
      Wu H.T. , Huang M.C.
      Submitted (2002-08) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];VARIANT THR-214
    5. 5.
      NIH - Mammalian Gene Collection (MGC) project
      Submitted (2007-06) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA];VARIANT THR-214
      strain: Hereford.
      tissue: Fetal liver.
      tissue: Testis.
    6. 6.
      "Biosynthesis of bovine plasma proteins in a cell-free system. Amino-terminal sequence of preproalbumin."
      McGillivray R.T.A. , Chung D.W. , Davie E.W.
      Eur. J. Biochem.98:477-485(1979) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 1-32
    7. 7.
      "Bovine microsomal albumin: amino terminal sequence of bovine proalbumin."
      Patterson J.E. , Geller D.M.
      Biochem. Biophys. Res. Commun.74:1220-1226(1977) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 19-28
    8. 8.
      "Rapid confirmation and revision of the primary structure of bovine serum albumin by ESIMS and Frit-FAB LC/MS."
      Hirayama K. , Akashi S. , Furuya M. , Fukuhara K.
      Biochem. Biophys. Res. Commun.173:639-646(1990) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE;SEQUENCE REVISION TO 118-119 AND 180
    9. 9.
      "Structure of bovine serum albumin."
      Brown J.R.
      Fed. Proc.34:591-591(1975)
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 25-424 AND 429-607;VARIANT THR-214
    10. 10.
      Brown J.R.
      Submitted (1975-04) to the PIR data bank
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SEQUENCE REVISION TO 190-195
    11. 11.
      "Tooth 'enamelins' identified mainly as serum proteins. Major 'enamelin' is albumin."
      Strawich E. , Glimcher M.J.
      Eur. J. Biochem.191:47-56(1990) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 25-64
    12. 12.
      "Electroblotting onto glass-fiber filter from an analytical isoelectrofocusing gel: a preparative method for isolating proteins for N-terminal microsequencing."
      Hsieh J.C. , Lin F.P. , Tam M.F.
      Anal. Biochem.170:1-8(1988) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 25-41
    13. 13.
      "Structures of histamine-releasing peptides formed by the action of acid proteases on mammalian albumin(s)."
      Carraway R.E. , Cochrane D.E. , Boucher W. , Mitra S.P.
      J. Immunol.143:1680-1684(1989) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 163-172
    14. 14.
      "Structure of a biologically active neurotensin-related peptide obtained from pepsin-treated albumin(s)."
      Carraway R.E. , Mitra S.P. , Cochrane D.E.
      J. Biol. Chem.262:5968-5973(1987) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 165-173
      tissue: Plasma.
    15. 15.
      "Sequence of residues 400-403 of bovine serum albumin."
      Reed R.G. , Putnam F.W. , Peters T. Jr.
      Biochem. J.191:867-868(1980) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 402-433
    16. 16.
      Vilbois F.
      Submitted (1998-08) to the UniProtKB
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 437-451
    17. 17.
      "Structure of serum albumin: disulfide bridges."
      Brown J.R.
      Fed. Proc.33:1389-1389(1974)
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DISULFIDE BONDS
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