Connective tissue growth factor (Protein name
), CTGF_RAT from NCBI database.
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General Annotation
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Antigen Annotation
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3D
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Predicted Eptitope
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Vaild Sequence
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Related Databases
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Citations (1)
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Gene name:
Ctgf(Ccn2);
Protein name:
Connective tissue growth factor;
Alternative:
Connective tissue growth-related protein;CCN family member 2;
Organism:
Rat (Rattus norvegicus).
General Annotation
Sub Unit:
N/A
Function:
Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes (By similarity). Mediates heparin-and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells (By similarity). Enhances fibroblast growth factor-induced DNA synthesis.
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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.0%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.8%
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Recovery
Recovery was determined by spiking various levels of CTGF into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
99
95-103
Plasma
98
96-101
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Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CTGF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
98-105%
100-106%
92-98%
92-103%
EDTA plasma(n=5)
89-98%
93-100%
95-102%
96-103%
heparin plasma(n=5)
99-104%
96-105%
101-108%
92-99%
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