SKP2 CLIA Kit for Human(Homo sapiens) | EIAab.comSKP2 CLIA Kit for Human(Homo sapiens)

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SKP2 (Gene name),

S-phase kinase-associated protein 2 (Protein name ), SKP2_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

SKP2(FBXL1);

Protein name:

S-phase kinase-associated protein 2;

Alternative:

F-box protein Skp2;Cyclin-A/CDK2-associated protein p45;p45skp2;F-box/LRR-repeat protein 1;

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Part of a SCF(SKP2) complex consisting of CUL1, RBX1, SKP1 and SKP2. Component of a SCF(SKP2)-like complex containing CUL1, SKP1, TRIM21 and SKP2. Interacts directly with CUL1 and SKP1. Interacts with CKS1. Interacts with the cyclin-A-CDK2 complex. Interacts with ORC1, phosphorylated CDT1, phosphorylated RBL2, ELF4, phosphorylated RAG2, FOXO1A, UBP43, MYC, TOB1, TAL1 and MLL. Interacts with TRIM21.

Function:

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, MLL, CDK9, RAG2, FOXO1A, UBP43, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2.

Subcellular Location:

N/A

Protein Attributes:

Sequence length:

424

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

424:

IGNKKNQEIW | GIKCRLTLQK | PSCL

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

UniGene:

Hs.23348

SMR:

Q13309

KEGG:

hsa:6502

STRING:

9606.ENSP00000274255

MIM:

601436

String:

Q13309

Uniprot:

Q13309

FOR

ELISA Kit for Human S-phase kinase-associated protein 2

Cat.:

E9870h

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96T

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ELISA Kit for Human S-phase kinase-associated protein 2

Cat.:

E9870m

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Packing:

96T

CLIA Kit for Human S-phase kinase-associated protein 2

Cat.:

U9870h

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MSDS:

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Packing:

96T

CLIA Kit for Human S-phase kinase-associated protein 2

Cat.:

U9870m

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MSDS:

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Packing:

96T

Polyclonal Antibody for Human S-phase kinase-associated protein 2

Cat.:

P9870Rb-m

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human S-phase kinase-associated protein 2

Cat.:

P9870Rb-h

Price:

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Packing:

40ug/0.2ml

Monoclonal Antibody for Human S-phase kinase-associated protein 2

Protein for Human S-phase kinase-associated protein 2

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.1%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.6%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	100	92-103
Plasma	103	95-107

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"p19Skp1 and p45Skp2 are essential elements of the cyclin A-CDK2 S phase kinase."
Zhang H. , Kobayashi R. , Galaktionov K. , Beach D.
Cell82:915-925(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);PROTEIN SEQUENCE OF 6-19; 31-58; 80-86 AND 365-372;INTERACTION WITH CYCLIN A-CDK2 COMPLEX
2.

"Human SKP2-like protein."
Yamaguchi T.
Submitted (2000-11) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2)
tissue: Liver.
3.

"Androgenic regulation of Skp2 in androgen-dependent and -independent LNCaP human prostate tumor cells."
Kokontis J.M. , Fukuchi J. , Liao S.
Submitted (2001-04) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
tissue: Prostatic carcinoma.
4.

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3)
tissue: Thalamus.
5.

"The DNA sequence and comparative analysis of human chromosome 5."
Schmutz J. , Martin J. , Terry A. , Couronne O. , Grimwood J. , Lowry S. , Gordon L.A. , Scott D. , Xie G. , Huang W. , Hellsten U. , Tran-Gyamfi M. , She X. , Prabhakar S. , Aerts A. , Altherr M. , Bajorek E. , Black S. , more...
Nature431:268-274(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
6.

Mural R.J. , Istrail S. , Sutton G.G. , Florea L. , Halpern A.L. , Mobarry C.M. , Lippert R. , Walenz B. , Shatkay H. , Dew I. , Miller J.R. , Flanigan M.J. , Edwards N.J. , Bolanos R. , Fasulo D. , Halldorsson B.V. , Hannenhalli S. , Turner R. , more...
Submitted (2005-07) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
7.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2)
tissue: Placenta.
8.

"The pRb-related protein p130 is regulated by phosphorylation-dependent proteolysis via the protein-ubiquitin ligase SCF(Skp2)."
Tedesco D. , Lukas J. , Reed S.I.
Genes Dev.16:2946-2957(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH RBL2;FUNCTION IN UBIQUITINATION OF RBL2
9.

"Human origin recognition complex large subunit is degraded by ubiquitin-mediated proteolysis after initiation of DNA replication."
Mendez J. , Zou-Yang X.H. , Kim S.Y. , Hidaka M. , Tansey W.P. , Stillman B.
Mol. Cell9:481-491(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH ORC1;FUNCTION IN UBIQUITINATION OF ORC1
10.

"The SCF(Skp2) ubiquitin ligase complex interacts with the human replication licensing factor Cdt1 and regulates Cdt1 degradation."
Li X. , Zhao Q. , Liao R. , Sun P. , Wu X.
J. Biol. Chem.278:30854-30858(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH CDT1;FUNCTION IN UBIQUITINATION OF CDT1
11.

"The F-box protein Skp2 participates in c-Myc proteosomal degradation and acts as a cofactor for c-Myc-regulated transcription."
von der Lehr N. , Johansson S. , Wu S. , Bahram F. , Castell A. , Cetinkaya C. , Hydbring P. , Weidung I. , Nakayama K. , Nakayama K.I. , Soderberg O. , Kerppola T.K. , Larsson L.G.
Mol. Cell11:1189-1200(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH MYC;FUNCTION IN UBIQUITINATION OF MYC
12.

"The ISG15 isopeptidase UBP43 is regulated by proteolysis via the SCFSkp2 ubiquitin ligase."
Tokarz S. , Berset C. , La Rue J. , Friedman K. , Nakayama K. , Nakayama K. , Zhang D.E. , Lanker S.
J. Biol. Chem.279:46424-46430(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH UBP43;FUNCTION IN UBIQUITINATION OF UBP43
13.

"Ubiquitination of p21Cip1/WAF1 by SCFSkp2: substrate requirement and ubiquitination site selection."
Wang W. , Nacusi L. , Sheaff R.J. , Liu X.
Biochemistry44:14553-14564(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN UBIQUITINATION OF CDKN1A
14.

"Ubiquitylation of RAG-2 by Skp2-SCF links destruction of the V(D)J recombinase to the cell cycle."
Jiang H. , Chang F.C. , Ross A.E. , Lee J. , Nakayama K. , Nakayama K. , Desiderio S.
Mol. Cell18:699-709(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH RAG2;FUNCTION IN UBIQUITINATION OF RAG2
15.

"Ubiquitylation of Cdk9 by Skp2 facilitates optimal Tat transactivation."
Barboric M. , Zhang F. , Besenicar M. , Plemenitas A. , Peterlin B.M.
J. Virol.79:11135-11141(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN UBIQUITINATION OF CDK9
16.

"Skp2 inhibits FOXO1 in tumor suppression through ubiquitin-mediated degradation."
Huang H. , Regan K.M. , Wang F. , Wang D. , Smith D.I. , van Deursen J.M. , Tindall D.J.
Proc. Natl. Acad. Sci. U.S.A.102:1649-1654(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH FOXO1;FUNCTION IN UBIQUITINATION OF FOXO1
17.

"Degradation of Tob1 mediated by SCFSkp2-dependent ubiquitination."
Hiramatsu Y. , Kitagawa K. , Suzuki T. , Uchida C. , Hattori T. , Kikuchi H. , Oda T. , Hatakeyama S. , Nakayama K.I. , Yamamoto T. , Konno H. , Kitagawa M.
Cancer Res.66:8477-8483(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH TOB1;FUNCTION IN UBIQUITINATION OF TOB1
18.

"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V. , Blagoev B. , Gnad F. , Macek B. , Kumar C. , Mortensen P. , Mann M.
Cell127:635-648(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-64;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
19.

"The ETS protein MEF is regulated by phosphorylation-dependent proteolysis via the protein-ubiquitin ligase SCFSkp2."
Liu Y. , Hedvat C.V. , Mao S. , Zhu X.H. , Yao J. , Nguyen H. , Koff A. , Nimer S.D.
Mol. Cell. Biol.26:3114-3123(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH ELF4;FUNCTION IN UBIQUITINATION OF ELF4
20.

"Regulation of p27 degradation and S-phase progression by Ro52 RING finger protein."
Sabile A. , Meyer A.M. , Wirbelauer C. , Hess D. , Kogel U. , Scheffner M. , Krek W.
Mol. Cell. Biol.26:5994-6004(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH THE SCF(SKP2)-LIKE COMPLEX;INTERACTION WITH TRIM21
21.

"Bimodal degradation of MLL by SCFSkp2 and APCCdc20 assures cell cycle execution: a critical regulatory circuit lost in leukemogenic MLL fusions."
Liu H. , Cheng E.H. , Hsieh J.J.
Genes Dev.21:2385-2398(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH KMT2A/MLL1;FUNCTION IN UBIQUITINATION OF KMT2A/MLL1
22.

"Ubiquitination and degradation of Tal1/SCL are induced by Notch signaling and depend on Skp2 and CHIP."
Nie L. , Wu H. , Sun X.H.
J. Biol. Chem.283:684-692(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH TAL1;FUNCTION IN UBIQUITINATION OF TAL1
23.

"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
Cantin G.T. , Yi W. , Lu B. , Park S.K. , Xu T. , Lee J.-D. , Yates J.R. III
J. Proteome Res.7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-179;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
24.

"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H. , Olsen J.V. , Bairlein M. , Gnad F. , Oppermann F.S. , Korner R. , Greff Z. , Keri G. , Stemmann O. , Mann M.
Mol. Cell31:438-448(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-64;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
25.

"A quantitative atlas of mitotic phosphorylation."
Dephoure N. , Zhou C. , Villen J. , Beausoleil S.A. , Bakalarski C.E. , Elledge S.J. , Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A.105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-64;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
26.

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V. , Vermeulen M. , Santamaria A. , Kumar C. , Miller M.L. , Jensen L.J. , Gnad F. , Cox J. , Jensen T.S. , Nigg E.A. , Brunak S. , Mann M.
Sci. Signal.3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-64;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
27.

"Initial characterization of the human central proteome."
Burkard T.R. , Planyavsky M. , Kaupe I. , Breitwieser F.P. , Buerckstuemmer T. , Bennett K.L. , Superti-Furga G. , Colinge J.
BMC Syst. Biol.5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
28.

"Ubiquitin-recognition protein Ufd1 couples the endoplasmic reticulum (ER) stress response to cell cycle control."
Chen M. , Gutierrez G.J. , Ronai Z.A.
Proc. Natl. Acad. Sci. U.S.A.108:9119-9124(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DEUBIQUITINATION BY USP13
29.

"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T. , Prokhorova T.A. , Akimov V. , Henningsen J. , Johansen P.T. , Kratchmarova I. , Kassem M. , Mann M. , Olsen J.V. , Blagoev B.
Sci. Signal.4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-64;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
30.

"Acetylation-dependent regulation of Skp2 function."
Inuzuka H. , Gao D. , Finley L.W. , Yang W. , Wan L. , Fukushima H. , Chin Y.R. , Zhai B. , Shaik S. , Lau A.W. , Wang Z. , Gygi S.P. , Nakayama K. , Teruya-Feldstein J. , Toker A. , Haigis M.C. , Pandolfi P.P. , Wei W.
Cell150:179-193(2012) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;ACETYLATION AT LYS-68 AND LYS-71;NUCLEAR LOCALIZATION SIGNAL;SUBCELLULAR LOCATION
31.

"Insights into SCF ubiquitin ligases from the structure of the Skp1-Skp2 complex."
Schulman B.A. , Carrano A.C. , Jeffrey P.D. , Bowen Z. , Kinnucan E.R.E. , Finnin M.S. , Elledge S.J. , Harper J.W. , Pagano M. , Pavletich N.P.
Nature408:381-386(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 101-153 IN COMPLEX WITH 1-147 OF SKP1
32.

"Structure of the Cul1-Rbx1-Skp1-F box Skp2 SCF ubiquitin ligase complex."
Zheng N. , Schulman B.A. , Song L. , Miller J.J. , Jeffrey P.D. , Wang P. , Chu C. , Koepp D.M. , Elledge S.J. , Pagano M. , Conaway R.C. , Conaway J.W. , Harper J.W. , Pavletich N.P.
Nature416:703-709(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) IN COMPLEX WITH CUL1; SKP1 AND RBX1;LEUCINE-RICH REPEATS;INTERACTION WITH CKS1

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