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Index > Protein center > CYCS(Gene name) > Bovine
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  • CYCS (Gene name),
  • Cytochrome c (Protein name ),  CYC_BOVIN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Predicted Eptitope
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  • Gene name:
    CYCS(CYC);
    Protein name:
    Cytochrome c;
    Alternative:

    Organism:
    Bovine (Bos taurus). 
    General Annotation
    Sub Unit:
    N/A
    Function:
    Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
    Subcellular Location:
    Mitochondrion matrix
    Protein Attributes:
    Sequence length:
    105
    Sequence:
    50:
    MGDVEKGKKI | FVQKCAQCHT | VEKGGKHKTG | PNLHGLFGRK | TGQAPGFSYT | 
    100:
    DANKNKGITW | GEETLMEYLE | NPKKYIPGTK | MIFAGIKKKG | EREDLIAYLK | 
    105:
    KATNE
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    String:
    SMR:
    Pfam:
    UniGene:
    Uniprot:
     
    FOR
    ELISA Kit for Bovine Cytochrome c
    Cat.:
    E0594r
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    MSDS:
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    Packing:
    96T
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    ELISA Kit for Bovine Cytochrome c
    Cat.:
    E0594h
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    MSDS:
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    Packing:
    96T
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    ELISA Kit for Bovine Cytochrome c
    Cat.:
    E0594m
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    MSDS:
    Please sign in first.
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    96T
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    ELISA Kit for Bovine Cytochrome c
    Cat.:
    E0594d
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    MSDS:
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    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Bovine Cytochrome c
    Cat.:
    E0594b
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    MSDS:
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    Packing:
    96T
    Range:
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    ELISA Kit for Bovine Cytochrome c
    Cat.:
    E0594p
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    Please sign in first.
    Packing:
    96T
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    ELISA Kit for Bovine Cytochrome c
    MSDS:
    Please sign in first.
    CLIA Kit for Bovine Cytochrome c
    Cat.:
    U0594m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Bovine Cytochrome c
    Cat.:
    U0594d
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Bovine Cytochrome c
    MSDS:
    Please sign in first.
    CLIA Kit for Bovine Cytochrome c
    Cat.:
    U0594b
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    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Bovine Cytochrome c
    Cat.:
    U0594r
    Price:
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    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Bovine Cytochrome c
    Cat.:
    U0594p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Bovine Cytochrome c
    Cat.:
    U0594h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
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    Polyclonal Antibody for Bovine Cytochrome c
    Cat.:
    P0594Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Bovine Cytochrome c
    Polyclonal Antibody for Bovine Cytochrome c
    Polyclonal Antibody for Bovine Cytochrome c
    Polyclonal Antibody for Bovine Cytochrome c
    Polyclonal Antibody for Bovine Cytochrome c
    Cat.:
    P0594Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Bovine Cytochrome c
    Cat.:
    P0594Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Bovine Cytochrome c
    Monoclonal Antibody for Bovine Cytochrome c
    Monoclonal Antibody for Bovine Cytochrome c
    Monoclonal Antibody for Bovine Cytochrome c
    Monoclonal Antibody for Bovine Cytochrome c
    Monoclonal Antibody for Bovine Cytochrome c
    Monoclonal Antibody for Bovine Cytochrome c
    Protein for Bovine Cytochrome c
    Protein for Bovine Cytochrome c
    Protein for Bovine Cytochrome c
    Protein for Bovine Cytochrome c
    Protein for Bovine Cytochrome c
    Protein for Bovine Cytochrome c
    Protein for Bovine Cytochrome c

    R&D Technical Data
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    s

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    s
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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

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    Recovery
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    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

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    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    EDTA plasma(n=5)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    EDTA plasma(n=5)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

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    References
    1. 1.
      NIH - Mammalian Gene Collection (MGC) project
      Submitted (2005-09) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      strain: Hereford.
      tissue: Thymus.
    2. 2.
      "The amino acid sequence of bovine heart cytochrome c."
      Nakashima T. , Higa H. , Matsubara H. , Benson A.M. , Yasunobu K.T.
      J. Biol. Chem.241:1166-1177(1966) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 2-105;ACETYLATION AT GLY-2
      tissue: Heart.
    3. 3.
      "New prospects for an old enzyme: mammalian cytochrome c is tyrosine-phosphorylated in vivo."
      Lee I. , Salomon A.R. , Yu K. , Doan J.W. , Grossman L.I. , Huttemann M.
      Biochemistry45:9121-9128(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT TYR-49
      tissue: Heart.
    4. 4.
      "Mammalian liver cytochrome c is tyrosine-48 phosphorylated in vivo, inhibiting mitochondrial respiration."
      Yu H. , Lee I. , Salomon A.R. , Yu K. , Huttemann M.
      Biochim. Biophys. Acta1777:1066-1071(2008) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT TYR-98
      tissue: Liver.
    5. 5.
      "High resolution X-ray crystallographic structure of bovine heart cytochrome c and its application to the design of an electron transfer biosensor."
      Mirkin N. , Jaconcic J. , Stojanoff V. , Moreno A.
      Proteins70:83-92(2008) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 2-105;HEME-BINDING SITES
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